Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0007311
UniProt IDQ8TCJ2
Primary gene name(s)STT3B
Synonym gene name(s)SIMP
Protein nameDolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B
Protein functionCatalytic subunit of the N-oligosaccharyl transferase, OST complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum, ER. STT3B is present in a small subset of OST complexes and mediates both cotranslational and post-translational N-glycosylation of target proteins: STT3B-containing complexes are required for efficient cotranslational glycosylation and while they are less competent than STT3A-containing complexes for cotranslational glycosylation, they have the ability to mediate glycosylation of some nascent sites that are not accessible for STT3A. STT3B-containing complexes also act post-translationally and mediate modification of skipped glycosylation sites in unfolded proteins. Plays a role in ER-associated degradation, ERAD pathway that mediates ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins by mediating N-glycosylation of unfolded proteins, which are then recognized by the ERAD pathway and targeted for degradation. Mediates glycosylation of the disease variant AMYL-TTR 'Asp-38' of TTR at 'Asn-118', leading to its degradation. {ECO:0000269|PubMed:19167329, ECO:0000269|PubMed:22607976}.
Subcellular locationEndoplasmic reticulum membrane;
Multi-pass membrane protein.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q8TCJ2
Gene Ontology
(Biological Process)
Complete annatation
co-translational protein modification [GO:0043686];
ER-associated ubiquitin-dependent protein catabolic process [GO:0030433];
glycoprotein catabolic process [GO:0006516];
post-translational protein modification [GO:0043687];
protein N-linked glycosylation via asparagine [GO:0018279];
response to unfolded protein [GO:0006986]
Gene Ontology
(Molecular Function)
Complete annatation
dolichyl-diphosphooligosaccharide-protein glycotransferase activity [GO:0004579]
Gene Ontology
(Cellular Component)
Complete annatation
endoplasmic reticulum [GO:0005783];
integral component of membrane [GO:0016021];
membrane [GO:0016020];
oligosaccharyltransferase complex [GO:0008250]
Protein-protein interaction128394
Phylogenetic treeQ8TCJ2
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.3446873054866120.2920186109128470.409499860781394
AZA vs. DISU0.03743927398081320.8823358864034570.991771899371402
AZA vs. IL70.4194230206784150.08231568621343730.792807883894659
AZA vs. SAHA0.05661499510703160.8162754018730540.953102139849797
DISU vs. CD3-0.3199385566026280.3782343123171020.512679077768906
DISU vs. IL70.3737029312524230.139189397059220.486844881121906
DISU vs. SAHA0.01930201966782270.9474616517222170.988308743958657
DMSO vs. AZA-0.1309896270731460.4324959281979951
DMSO vs. CD3-0.4885685834724030.1266326970735560.206664671369638
DMSO vs. DISU-0.1707622131365450.4844181210471090.902712522924543
DMSO vs. IL70.5579540138494890.006894820522000520.173378076038115
DMSO vs. SAHA0.1800899893759620.4444103817911880.776743605602472
HIV vs. Mock in Activation-0.1577084579092670.799873228314410.999983755607037
HIV vs. Mock in Latency-0.03822710978151790.816080293494930.999834320637052
IL7 vs. CD30.08465381406883550.7917273769901460.862823133061418
SAHA vs. CD3-0.3141028037153410.3740424798434110.49067783219262
SAHA vs. IL7-0.367122864177810.1313799562895610.338591461977917
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.308674 0.0178891
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.037 0.992 1.038 1.155 0.991
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00510 N-Glycan biosynthesis - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa04141 Protein processing in endoplasmic reticulum - Homo sapiens (human)