Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0007284
UniProt IDO95630
Primary gene name(s)STAMBP
Synonym gene name(s)AMSH
Protein nameSTAM-binding protein
Protein functionZinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not cleave 'Lys-48'-linked polyubiquitin chains, By similarity. Plays a role in signal transduction for cell growth and MYC induction mediated by IL-2 and GM-CSF. Potentiates BMP, bone morphogenetic protein signaling by antagonizing the inhibitory action of SMAD6 and SMAD7. Has a key role in regulation of cell surface receptor-mediated endocytosis and ubiquitin-dependent sorting of receptors to lysosomes. Endosomal localization of STAMBP is required for efficient EGFR degradation but not for its internalization, By similarity. Involved in the negative regulation of PI3K-AKT-mTOR and RAS-MAP signaling pathways. {ECO:0000250, ECO:0000269|PubMed:10383417, ECO:0000269|PubMed:11483516, ECO:0000269|PubMed:15314065, ECO:0000269|PubMed:17261583, ECO:0000269|PubMed:23542699}.
Subcellular locationNucleus. Membrane;
Peripheral membrane protein. Cytoplasm. Early endosome.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O95630
Gene Ontology
(Biological Process)
Complete annatation
JAK-STAT cascade [GO:0007259];
mitotic cytokinesis [GO:0000281];
negative regulation of neuron apoptotic process [GO:0043524];
negative regulation of phosphatidylinositol 3-kinase signaling [GO:0014067];
negative regulation of Ras protein signal transduction [GO:0046580];
positive regulation of cell proliferation [GO:0008284];
protein deubiquitination [GO:0016579]
Gene Ontology
(Molecular Function)
Complete annatation
metal ion binding [GO:0046872];
metallopeptidase activity [GO:0008237];
protein domain specific binding [GO:0019904];
thiol-dependent ubiquitin-specific protease activity [GO:0004843]
Gene Ontology
(Cellular Component)
Complete annatation
cleavage furrow [GO:0032154];
cytoplasm [GO:0005737];
early endosome [GO:0005769];
extracellular exosome [GO:0070062];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
plasma membrane [GO:0005886]
Protein-protein interaction115863
Phylogenetic treeO95630
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.7298112901954950.02715480501133840.0599497907032213
AZA vs. DISU0.09529779092184680.7075722503052730.972282962725769
AZA vs. IL7-0.0161299637895860.9337331324348540.999311006273513
AZA vs. SAHA-0.03100972411826820.8994557864485260.977463522811988
DISU vs. CD3-0.6476025568800980.0754703884292640.149453577927316
DISU vs. IL7-0.1207182478926290.6330861981955490.890526342452006
DISU vs. SAHA-0.1246219564150760.6697193779087230.899352772200297
DMSO vs. AZA0.04485066722957240.7913988251904971
DMSO vs. CD3-0.6969996734775390.03060033869001090.0638672904357182
DMSO vs. DISU-0.05239873996661960.8306253232852440.98111438763097
DMSO vs. IL7-0.05382130556957070.7667595059846730.952362365362771
DMSO vs. SAHA-0.08217406195849130.7288818429979310.920246146923164
HIV vs. Mock in Activation-0.1195602607561030.8476577969854830.999983755607037
HIV vs. Mock in Latency0.02961195032380880.858735456919950.999834320637052
IL7 vs. CD3-0.7387281474264030.0224965389417060.0574826803641862
SAHA vs. CD3-0.785644760551490.02723842130118540.0596805770585728
SAHA vs. IL7-0.01831942435586630.9403371434704920.977291926411624
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.330756 0.0171733
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.018 0.95 1.053 1.114 0.996
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2XZE X-ray 1.7Å A/B=1-146.
3RZU X-ray 2.5Å A/B/C/D/E/F/G=243-424.
3RZV X-ray 1.6Å A=219-424.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04144 Endocytosis - Homo sapiens (human)