Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0007269
UniProt IDQ13243
Primary gene name(s)SRSF5
Synonym gene name(s)HRS, SFRS5, SRP40
Protein nameSerine/arginine-rich splicing factor 5
Protein functionPlays a role in constitutive splicing and can modulate the selection of alternative splice sites.
Subcellular locationNucleus.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q13243
Gene Ontology
(Biological Process)
Complete annatation
cellular response to insulin stimulus [GO:0032869];
liver regeneration [GO:0097421];
mRNA 3'-end processing [GO:0031124];
mRNA export from nucleus [GO:0006406];
mRNA processing [GO:0006397];
mRNA splice site selection [GO:0006376];
mRNA splicing, via spliceosome [GO:0000398];
positive regulation of RNA splicing [GO:0033120];
regulation of cell cycle [GO:0051726];
response to wounding [GO:0009611];
RNA export from nucleus [GO:0006405];
termination of RNA polymerase II transcription [GO:0006369]
Gene Ontology
(Molecular Function)
Complete annatation
nucleotide binding [GO:0000166];
poly(A RNA binding [GO:0044822]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
nuclear speck [GO:0016607];
nucleolus [GO:0005730];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction112328
Phylogenetic treeQ13243
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.7628749532631470.02028534849147330.0471313045265124
AZA vs. DISU-0.150650238727530.5517628512154380.946928765276725
AZA vs. IL7-0.2477826198376050.1961850833227040.959507283306048
AZA vs. SAHA-0.3248729353692750.1827048887997480.545562582452314
DISU vs. CD30.5984684334360130.1009131744065480.187701860842613
DISU vs. IL7-0.1060149304741390.6734879863334160.910649510626626
DISU vs. SAHA-0.172707408006660.5561180808137090.85401014835372
DMSO vs. AZA0.01106643371636850.9470661172044481
DMSO vs. CD30.761635627928570.01768310404588350.0403019610364954
DMSO vs. DISU0.1597138055920840.512695155437990.912102132233928
DMSO vs. IL7-0.2515942010963520.1603870083424710.666759266808878
DMSO vs. SAHA-0.3427011673346260.1461058173132290.460386613981128
HIV vs. Mock in Activation-0.02378511504437760.9694874550683770.999983755607037
HIV vs. Mock in Latency0.02568758507306020.8757067795942360.999834320637052
IL7 vs. CD30.5230020414602350.1040833672060280.195580146081333
SAHA vs. CD30.4133875336858260.2419225943942540.350252041264426
SAHA vs. IL7-0.08123549730403930.7387629333184910.881300447676712
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
0.7419 0.03511

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
-1.4 0.032113199 -1.6 0.000590725 -1.8 0.000778947
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.0376701 0.844259
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.259 0.99 0.861 0.882 0.8
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
210077_s_at 1.54 No downregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Pr55(Gag) upregulates 20427542
Nef regulated by 15163745
Vpu regulated by 15163745
capsid upregulated by 22933280
Rev requires 15163745
Envelope surface glycoprotein gp160; precursor regulated by 15163745

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03040 Spliceosome - Homo sapiens (human)
hsa05168 Herpes simplex infection - Homo sapiens (human)