Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0007266
UniProt IDO75494
Primary gene name(s)SRSF10
Synonym gene name(s)FUSIP1, FUSIP2, SFRS13A, TASR
Protein nameSerine/arginine-rich splicing factor 10
Protein functionSplicing factor that in its dephosphorylated form acts as a general repressor of pre-mRNA splicing, PubMed:11684676, PubMed:12419250, PubMed:14765198. Seems to interfere with the U1 snRNP 5'-splice recognition of SNRNP70, PubMed:14765198. Required for splicing repression in M-phase cells and after heat shock, PubMed:14765198. Also acts as a splicing factor that specifically promotes exon skipping during alternative splicing, PubMed:26876937. Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine, m6A-containing RNAs, prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing, PubMed:26876937. May be involved in regulation of alternative splicing in neurons, with isoform 1 acting as a positive and isoform 3 as a negative regulator, PubMed:12419250. {ECO:0000269|PubMed:11684676, ECO:0000269|PubMed:12419250, ECO:0000269|PubMed:14765198, ECO:0000269|PubMed:26876937}.
Subcellular locationNucleus speckle {ECO:0000269|PubMed:11684676, ECO:0000269|PubMed:26876937}. Cytoplasm {ECO:0000269|PubMed:11684676}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O75494
Gene Ontology
(Biological Process)
Complete annatation
cytosolic transport [GO:0016482];
mRNA export from nucleus [GO:0006406];
mRNA splice site selection [GO:0006376];
mRNA splicing, via spliceosome [GO:0000398];
negative regulation of mRNA splicing, via spliceosome [GO:0048025];
regulation of mRNA splicing, via spliceosome [GO:0048024];
regulation of transcription, DNA-templated [GO:0006355];
RNA splicing, via transesterification reactions [GO:0000375];
spliceosomal tri-snRNP complex assembly [GO:0000244]
Gene Ontology
(Molecular Function)
Complete annatation
nucleotide binding [GO:0000166];
poly(A RNA binding [GO:0044822];
RNA binding [GO:0003723];
RS domain binding [GO:0050733];
unfolded protein binding [GO:0051082]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
nuclear speck [GO:0016607];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction115990
Phylogenetic treeO75494
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.239356334830680.0001869321036841450.00083578779790194
AZA vs. DISU0.06522718666812820.7961346247926450.981946742545343
AZA vs. IL70.2702167120061560.1590959234986630.92284007403915
AZA vs. SAHA-0.02494930533474320.9184237118314260.981953943134223
DISU vs. CD3-1.18765575857930.001207814463382030.00487198159513532
DISU vs. IL70.1963620944027560.4349163664419210.787644578701608
DISU vs. SAHA-0.089164160180360.7594052113369810.932068474683465
DMSO vs. AZA0.0103098984959510.9507874876183851
DMSO vs. CD3-1.240977785553150.0001277042306826460.000559913921779412
DMSO vs. DISU-0.05675253287664790.8157349357194020.980200572985704
DMSO vs. IL70.2671698915343030.1365588852106660.634203695178923
DMSO vs. SAHA-0.04221850023358250.8576530656969870.962663986975444
HIV vs. Mock in Activation-0.01130602445996680.9854887288908860.999983755607037
HIV vs. Mock in Latency-0.004324313236851370.9790277647657510.999834320637052
IL7 vs. CD3-0.9607492751511480.002992089638003130.0108595634754686
SAHA vs. CD3-1.289307815166870.0003197509924689480.00132854906438022
SAHA vs. IL7-0.2992747157247310.2189407880200820.457741114925757
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.369839 0.0077978
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.98 1.021 0.904 0.865 0.985
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp120 complexes with 23125841
Gag-Pol complexes with 23125841
Pr55(Gag) complexes with 23125841
Tat upregulates 23166591
Nef complexes with 23125841

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03040 Spliceosome - Homo sapiens (human)