Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0007241
UniProt IDP16150
Primary gene name(s)SPN
Synonym gene name(s)CD43
Protein nameLeukosialin
Protein functionOne of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1, By similarity. During T-cell activation is actively removed from the T-cell-APC, antigen-presenting cell contact site thus suggesting a negative regulatory role in adaptive immune response, By similarity. {ECO:0000250}.
Subcellular locationMembrane;
Single-pass type I membrane protein.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P16150
Gene Ontology
(Biological Process)
Complete annatation
apoptotic signaling pathway [GO:0097190];
cell surface receptor signaling pathway [GO:0007166];
cellular defense response [GO:0006968];
chemotaxis [GO:0006935];
defense response to bacterium [GO:0042742];
establishment or maintenance of cell polarity [GO:0007163];
immune response [GO:0006955];
leukocyte migration [GO:0050900];
negative regulation of cell adhesion [GO:0007162];
negative regulation of T cell proliferation [GO:0042130];
negative regulation of type IV hypersensitivity [GO:0001808];
negative thymic T cell selection [GO:0045060];
positive regulation of protein phosphorylation [GO:0001934];
positive regulation of T cell proliferation [GO:0042102];
positive regulation of tumor necrosis factor biosynthetic process [GO:0042535];
regulation of defense response to virus [GO:0050688];
response to protozoan [GO:0001562];
signal transduction [GO:0007165];
T cell costimulation [GO:0031295]
Gene Ontology
(Molecular Function)
Complete annatation
transmembrane signaling receptor activity [GO:0004888]
Gene Ontology
(Cellular Component)
Complete annatation
basement membrane [GO:0005604];
cell surface [GO:0009986];
external side of plasma membrane [GO:0009897];
extracellular exosome [GO:0070062];
extracellular space [GO:0005615];
integral component of plasma membrane [GO:0005887];
membrane [GO:0016020];
plasma membrane [GO:0005886];
uropod [GO:0001931]
Protein-protein interaction112571
Phylogenetic treeP16150
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.046683077954090.001538854379474320.00529431948846994
AZA vs. DISU-0.6015061986495990.0175746837595030.317599368929949
AZA vs. IL70.2263811650248770.2377333752994790.990920320206469
AZA vs. SAHA-0.3955728798187040.1046523676120330.411136249513628
DISU vs. CD30.4342021118038230.2315610620463350.355706863271828
DISU vs. IL70.8183741424021390.001232496052915870.0313527434035721
DISU vs. SAHA0.2071882949282610.4768482483541680.808571101591546
DMSO vs. AZA0.03139768207601510.8507909439752871
DMSO vs. CD31.06728018525730.0009422839315851310.00324988723198947
DMSO vs. DISU0.6313558567474090.009890043651034080.213528592866882
DMSO vs. IL70.2020525264525710.2595801572913970.761613637829468
DMSO vs. SAHA-0.4334869628099560.06578397312548120.297013259129457
HIV vs. Mock in Activation0.2094598716374510.7361846707829470.999983755607037
HIV vs. Mock in Latency0.1357937132556950.4087645008605720.999834320637052
IL7 vs. CD31.279952600651198.50065153288337e-050.000504828739847632
SAHA vs. CD30.6269949831258560.07654614988868260.140539775684996
SAHA vs. IL7-0.6251939414486050.01034869281730460.0638805059492419
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.847978 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.012 0.986 0.589 0.462 1.142
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
-0.73 0.1745 0.07 0.842 -1.73 0.0002 T-cell activation at 20 hpi
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vpu antagonizes 25980612
Envelope surface glycoprotein gp120 downregulates 23867815
Vpr antagonizes 25980612
Envelope surface glycoprotein gp120 interacts with 8554898
Pr55(Gag) co-localizes with 21060818
Pr55(Gag) associates with 23536680
Nef antagonizes 25980612

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04514 Cell adhesion molecules (CAMs) - Homo sapiens (human)
hsa05169 Epstein-Barr virus infection - Homo sapiens (human)