Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0007214
UniProt IDQ99523
Primary gene name(s)SORT1
Synonym gene name(s)unknown
Protein nameSortilin
Protein functionFunctions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor, M6PR. Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF, proBDNF and NGFB, proNGFB. Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4, GLUT4 storage vesicles, or GSVs. These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi. {ECO:0000269|PubMed:10085125, ECO:0000269|PubMed:11331584, ECO:0000269|PubMed:11390366, ECO:0000269|PubMed:12209882, ECO:0000269|PubMed:12598608, ECO:0000269|PubMed:14657016, ECO:0000269|PubMed:14985763, ECO:0000269|PubMed:15313463, ECO:0000269|PubMed:15930396, ECO:0000269|PubMed:15987945}.
Subcellular locationMembrane;
Single-pass type I membrane protein. Endoplasmic reticulum membrane {ECO:0000305};
Single-pass type I membrane protein {ECO:0000305}. Endosome membrane {ECO:0000305};
Single-pass type I membrane protein {ECO:0000305}. Golgi apparatus, Golgi stack membrane {ECO:0000305};
Single-pass type I membrane protein {ECO:0000305}. Nucleus membrane {ECO:0000305};
Single-pass type I membrane protein {ECO:0000305}. Cell membrane;
Single-pass type I membrane protein;
Extracellular side. Lysosome membrane {ECO:0000305};
Single-pass type I membrane protein {ECO:0000305}. Note=Localized to membranes of the endoplasmic reticulum, endosomes, Golgi stack, lysosomes and nucleus. A small fraction of the protein is also localized to the plasma membrane. May also be found in SLC2A4/GLUT4 storage vesicles, GSVs in adipocytes. Localization to the plasma membrane in adipocytes may be enhanced by insulin.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q99523
Gene Ontology
(Biological Process)
Complete annatation
endocytosis [GO:0006897];
endosome to lysosome transport [GO:0008333];
endosome transport via multivesicular body sorting pathway [GO:0032509];
extrinsic apoptotic signaling pathway via death domain receptors [GO:0008625];
glucose import [GO:0046323];
Golgi to endosome transport [GO:0006895];
G-protein coupled receptor signaling pathway [GO:0007186];
multicellular organism development [GO:0007275];
myotube differentiation [GO:0014902];
negative regulation of fat cell differentiation [GO:0045599];
negative regulation of lipoprotein lipase activity [GO:0051005];
neuropeptide signaling pathway [GO:0007218];
neurotrophin TRK receptor signaling pathway [GO:0048011];
ossification [GO:0001503];
plasma membrane to endosome transport [GO:0048227];
positive regulation of epithelial cell apoptotic process [GO:1904037];
regulation of gene expression [GO:0010468];
response to insulin [GO:0032868];
vesicle organization [GO:0016050]
Gene Ontology
(Molecular Function)
Complete annatation
enzyme binding [GO:0019899];
nerve growth factor binding [GO:0048406];
nerve growth factor receptor activity [GO:0010465];
neurotensin receptor activity, non-G-protein coupled [GO:0030379]
Gene Ontology
(Cellular Component)
Complete annatation
cell surface [GO:0009986];
clathrin-coated vesicle [GO:0030136];
coated pit [GO:0005905];
cytoplasmic, membrane-bounded vesicle [GO:0016023];
cytosol [GO:0005829];
dendrite [GO:0030425];
early endosome [GO:0005769];
endoplasmic reticulum membrane [GO:0005789];
endosome membrane [GO:0010008];
Golgi apparatus [GO:0005794];
Golgi cisterna membrane [GO:0032580];
integral component of membrane [GO:0016021];
lysosomal membrane [GO:0005765];
neuronal cell body [GO:0043025];
nuclear membrane [GO:0031965];
perinuclear region of cytoplasm [GO:0048471];
plasma membrane [GO:0005886];
trans-Golgi network transport vesicle [GO:0030140]
Protein-protein interaction112180
Phylogenetic treeQ99523
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-2.317828231259451.65241599603405e-061.22356227477303e-05
AZA vs. DISU-0.1580637271696070.7139441998545110.973233942294547
AZA vs. IL70.7956555911983970.01349448878422290.386387698916739
AZA vs. SAHA0.2675754184245570.5006155210047390.821311269581892
DISU vs. CD32.150485659648323.49058783214451e-050.000224571579094094
DISU vs. IL70.9427356849258080.01198563604814810.130886003261952
DISU vs. SAHA0.4290714968665730.3293329402087130.710382093644071
DMSO vs. AZA-0.05525853572834840.8872573040196951
DMSO vs. CD32.260624431282385.54376554773306e-063.38535986933922e-05
DMSO vs. DISU0.103687791532240.8125549064967670.979344399543159
DMSO vs. IL70.8568076112106720.00916617624326910.199106782229512
DMSO vs. SAHA0.3165521278680480.4322476134052340.76790957037005
HIV vs. Mock in Activation0.7779061631108310.2373355511977130.999983755607037
HIV vs. Mock in Latency0.2856451501653260.3970015520894780.999834320637052
IL7 vs. CD33.11617706841074.73621142305092e-131.45989600776373e-11
SAHA vs. CD32.564054312394043.2297947338833e-072.96605768675328e-06
SAHA vs. IL7-0.5271437801734860.1177533082598050.317136567573395
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.950755 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.854 1.022 1.253 1.15 1.359
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3F6K X-ray 2.0Å A=78-756.
3G2U X-ray 2.3Å C/D=819-831.
3G2V X-ray 2.1Å C/D=819-831.
4MSL X-ray 2.7Å A=78-756.
4N7E X-ray 2.7Å A=78-756.
4PO7 X-ray 2.6Å A=78-756.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04142 Lysosome - Homo sapiens (human)
hsa04722 Neurotrophin signaling pathway - Homo sapiens (human)