Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0007207
UniProt IDO60493
Primary gene name(s)SNX3
Synonym gene name(s)unknown
Protein nameSorting nexin-3
Protein functionPhosphoinositide-binding protein required for multivesicular body formation. Specifically binds phosphatidylinositol 3-phosphate, PtdIns(P3. Also can bind phosphatidylinositol 4-phosphate, PtdIns(P4, phosphatidylinositol 5-phosphate, PtdIns(P5 and phosphatidylinositol 3,5-biphosphate, PtdIns(3,5P2, By similarity. Plays a role in protein transport between cellular compartments. Together with RAB7A facilitates endosome membrane association of the retromer cargo-selective subcomplex, CSC/VPS. May in part act as component of the SNX3-retromer complex which mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway, PubMed:21725319, PubMed:24344282. Promotes stability and cell surface expression of epithelial sodium channel, ENAC subunits SCNN1A and SCNN1G, By similarity. Not involved in EGFR degradation. Involved in the regulation of phagocytosis in dendritic cells possibly by regulating EEA1 recruitment to the nascent phagosomes, PubMed:23237080. Involved in iron homeostasis through regulation of endocytic recycling of the transferrin receptor TFRC presumably by delivering the tranferrin:transferrin receptor complex to recycling endosomes; the function may involve the CSC retromer subcomplex, By similarity. In the case of Salmonella enterica infection plays arole in maturation of the Salmonella-containing vacuole, SCV and promotes recruitment of LAMP1 to SCVs, PubMed:20482551. {ECO:0000250|UniProtKB:O70492, ECO:0000269|PubMed:11433298, ECO:0000269|PubMed:18767904, ECO:0000269|PubMed:21725319, ECO:0000269|PubMed:23237080, ECO:0000269|PubMed:24344282, ECO:0000305|PubMed:21725319}.
Subcellular locationEarly endosome {ECO:0000269|PubMed:11433298, ECO:0000269|PubMed:21725319}. Cytoplasmic vesicle, phagosome {ECO:0000269|PubMed:23237080}. Note=Colocalizes to clathrin-coated endosomal vesicles morphologically distinct from retromer-decorated non-branched endosomal tubule structures, PubMed:21725319 Colocalizes with EEA1 on nascent phagosomes in dendritic cells but competes with EEA1 for binding to phagosomal membrane, PubMed:23237080. In the case of Salmonella enterica infection localizes to Salmonella-containing vacuoles, SCVs from which SNX3-containing tubules form 30-60 min after infection, PubMed:20482551. {ECO:0000269|PubMed:20482551, ECO:0000269|PubMed:21725319, ECO:0000269|PubMed:23237080}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O60493
Gene Ontology
(Biological Process)
Complete annatation
intralumenal vesicle formation [GO:0070676];
membrane invagination [GO:0010324];
negative regulation of early endosome to late endosome transport [GO:2000642];
negative regulation of phagocytosis [GO:0050765];
negative regulation of protein catabolic process [GO:0042177];
negative regulation of protein transport [GO:0051224];
negative regulation of viral entry into host cell [GO:0046597];
positive regulation of neuron projection development [GO:0010976];
protein to membrane docking [GO:0022615];
protein transport [GO:0015031];
regulation of Wnt signaling pathway [GO:0030111];
response to bacterium [GO:0009617];
Wnt signaling pathway [GO:0016055]
Gene Ontology
(Molecular Function)
Complete annatation
phosphatidylinositol-3,5-bisphosphate binding [GO:0080025];
phosphatidylinositol-3-phosphate binding [GO:0032266];
phosphatidylinositol-4-phosphate binding [GO:0070273];
phosphatidylinositol-5-phosphate binding [GO:0010314];
protein phosphatase binding [GO:0019903]
Gene Ontology
(Cellular Component)
Complete annatation
clathrin-coated vesicle [GO:0030136];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
early endosome [GO:0005769];
early endosome membrane [GO:0031901];
early phagosome [GO:0032009];
endosome membrane [GO:0010008];
extracellular exosome [GO:0070062];
extrinsic component of membrane [GO:0019898]
Protein-protein interaction114263
Phylogenetic treeO60493
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.1806359840837260.5825450508316360.689285524111668
AZA vs. DISU-0.03637488473221790.8857028763002950.991918155184987
AZA vs. IL70.01404156451627280.9417944099339430.999311006273513
AZA vs. SAHA-0.003986021411531360.9869613849034270.99724510955636
DISU vs. CD30.1319512602831220.715814431717390.80093428917072
DISU vs. IL70.04144039241971980.8692911958752490.975103999887255
DISU vs. SAHA0.03329962084398240.9091329908678130.978778263230015
DMSO vs. AZA-0.07495833355847880.6548158637167151
DMSO vs. CD30.09334260924781430.7712949122735590.837017838745854
DMSO vs. DISU-0.04067666380699620.8677191087141210.985542797999996
DMSO vs. IL70.09631410635168560.5924851152035020.910191899693548
DMSO vs. SAHA0.0643570924982880.7848044771850760.939533582941956
HIV vs. Mock in Activation-0.2283734084090040.7136098127283330.999983755607037
HIV vs. Mock in Latency-0.05944542422478860.7186990089650030.999834320637052
IL7 vs. CD30.2030664506898750.5279930845808270.654706944157758
SAHA vs. CD30.1517058066618330.6694058937599910.754521078456475
SAHA vs. IL7-0.02168586773922540.9290767384617510.973712212721438
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.541639 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.98 0.995 0.973 1.126 1.033
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB04530 S,S-(2-Hydroxyethyl)Thiocysteine experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2MXC NMR - A=2-162.
2YPS X-ray 2.6Å A/B/C/D=24-155.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04144 Endocytosis - Homo sapiens (human)
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