Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0007206
UniProt IDO60749
Primary gene name(s)SNX2
Synonym gene name(s)unknown
Protein nameSorting nexin-2
Protein functionInvolved in several stages of intracellular trafficking. Interacts with membranes containing phosphatidylinositol 3-phosphate, PtdIns(3P or phosphatidylinositol 3,5-bisphosphate, PtdIns(3,5P2, PubMed:16179610. Acts in part as component of the retromer membrane-deforming SNX-BAR subcomplex, PubMed:17101778. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network, TGN and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX-BAR subcomplex functions to deform the donor membrane into a tubular profile called endosome-to-TGN transport carrier, ETC, Probable. Can sense membrane curvature and has in vitro vesicle-to-membrane remodeling activity, PubMed:23085988. Required for retrograde endosome-to-TGN transport of TGN38, PubMed:20138391. Promotes KALRN- and RHOG-dependent but retromer-independent membrane remodeling such as lamellipodium formation; the function is dependent on GEF activity of KALRN, PubMed:20604901. {ECO:0000269|PubMed:16179610, ECO:0000269|PubMed:17101778, ECO:0000269|PubMed:20138391, ECO:0000269|PubMed:20604901, ECO:0000269|PubMed:23085988, ECO:0000303|PubMed:16179610}.
Subcellular locationEarly endosome membrane {ECO:0000269|PubMed:16179610, ECO:0000269|PubMed:17101778};
Peripheral membrane protein {ECO:0000269|PubMed:16179610, ECO:0000269|PubMed:17101778};
Cytoplasmic side {ECO:0000269|PubMed:16179610, ECO:0000269|PubMed:17101778}. Cell projection, lamellipodium {ECO:0000269|PubMed:20604901}. Note=Colocalized with SORT1 to tubular endosomal membrane structures called endosome-to-TGN transport carriers, ETCs which are budding from early endosome vacuoles just before maturing into late endosome vacuoles, PubMed:18088323. Colocalized with F-actin at the leading edge of lamellipodia in cells in a KALRN-dependent manner, PubMed:20604901. {ECO:0000269|PubMed:18088323, ECO:0000269|PubMed:20604901}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O60749
Gene Ontology
(Biological Process)
Complete annatation
early endosome to Golgi transport [GO:0034498];
endocytosis [GO:0006897];
intracellular protein transport [GO:0006886];
lamellipodium morphogenesis [GO:0072673];
protein oligomerization [GO:0051259];
retrograde transport, endosome to Golgi [GO:0042147];
vesicle organization [GO:0016050]
Gene Ontology
(Molecular Function)
Complete annatation
epidermal growth factor receptor binding [GO:0005154];
insulin receptor binding [GO:0005158];
leptin receptor binding [GO:1990460];
phosphatidylinositol binding [GO:0035091];
protein heterodimerization activity [GO:0046982];
protein homodimerization activity [GO:0042803];
transferrin receptor binding [GO:1990459]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
early endosome membrane [GO:0031901];
endosome membrane [GO:0010008];
extracellular exosome [GO:0070062];
extrinsic component of membrane [GO:0019898];
lamellipodium [GO:0030027];
membrane [GO:0016020];
protein complex [GO:0043234];
retromer, tubulation complex [GO:0030905];
retromer complex [GO:0030904]
Protein-protein interaction112526
Phylogenetic treeO60749
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.2417276118693880.4637462913317680.583340352441362
AZA vs. DISU0.08207038732374860.7461719422328270.976008954784031
AZA vs. IL7-0.07737829244880160.6891187278423530.999311006273513
AZA vs. SAHA0.2198746949982140.3694451487259530.735833052269468
DISU vs. CD30.3108139857192870.3938134742312280.527768875405541
DISU vs. IL7-0.1679356773466990.5060176064353480.830044367655729
DISU vs. SAHA0.1382746290505350.6360509920201560.886761314059222
DMSO vs. AZA-0.1871255263937610.2684959398743371
DMSO vs. CD30.04131986031154230.8981436250473770.930736969901804
DMSO vs. DISU-0.2715398275337310.2675196234710150.785673683043636
DMSO vs. IL70.1170955851227220.5180568894830710.889301967358611
DMSO vs. SAHA0.399755121654980.09148857469393980.354271405244698
HIV vs. Mock in Activation-0.2874882645129190.644556027842990.999983755607037
HIV vs. Mock in Latency-0.09045054133681680.5864900132512820.999834320637052
IL7 vs. CD30.173997149905560.590134811363060.708208758891354
SAHA vs. CD30.4357180837189320.2241934151659290.330136510186868
SAHA vs. IL70.2927475178124370.231762748687860.472490083002542
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.701552 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.031 0.947 0.962 1 0.9
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04144 Endocytosis - Homo sapiens (human)