Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0007202
UniProt IDQ13596
Primary gene name(s)SNX1
Synonym gene name(s)unknown
Protein nameSorting nexin-1
Protein functionInvolved in several stages of intracellular trafficking. Interacts with membranes containing phosphatidylinositol 3-phosphate, PtdIns(3P or phosphatidylinositol 3,5-bisphosphate, PtdIns(3,5P2, PubMed:12198132. Acts in part as component of the retromer membrane-deforming SNX-BAR subcomplex. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network, TGN and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX-BAR subcomplex functions to deform the donor membrane into a tubular profile called endosome-to-TGN transport carrier, ETC, Probable. Can sense membrane curvature and has in vitro vesicle-to-membrane remodeling activity, PubMed:19816406, PubMed:23085988. Involved in retrograde endosome-to-TGN transport of lysosomal enzyme receptors, IGF2R, M6PR and SORT1 and Shiginella dysenteria toxin stxB. Plays a role in targeting ligand-activated EGFR to the lysosomes for degradation after endocytosis from the cell surface and release from the Golgi, PubMed:12198132, PubMed:15498486, PubMed:17550970, PubMed:17101778, PubMed:18088323, PubMed:21040701. Involvement in retromer-independent endocytic trafficking of P2RY1 and lysosomal degradation of protease-activated receptor-1/F2R, PubMed:16407403, PubMed:20070609. Promotes KALRN- and RHOG-dependent but retromer-independent membrane remodeling such as lamellipodium formation; the function is dependent on GEF activity of KALRN, PubMed:20604901. Required for endocytosis of DRD5 upon agonist stimulation but not for basal receptor trafficking, PubMed:23152498. {ECO:0000269|PubMed:12198132, ECO:0000269|PubMed:15498486, ECO:0000269|PubMed:16407403, ECO:0000269|PubMed:17101778, ECO:0000269|PubMed:17550970, ECO:0000269|PubMed:18088323, ECO:0000269|PubMed:19816406, ECO:0000269|PubMed:20070609, ECO:0000269|PubMed:20604901, ECO:0000269|PubMed:21040701, ECO:0000269|PubMed:23085988, ECO:0000269|PubMed:23152498, ECO:0000303|PubMed:15498486}.
Subcellular locationEndosome membrane {ECO:0000269|PubMed:15498486, ECO:0000269|PubMed:18088323, ECO:0000269|PubMed:22431521};
Peripheral membrane protein;
Cytoplasmic side. Golgi apparatus, trans-Golgi network membrane {ECO:0000305};
Peripheral membrane protein {ECO:0000305};
Cytoplasmic side {ECO:0000305}. Early endosome membrane;
Peripheral membrane protein;
Cytoplasmic side. Cell projection, lamellipodium {ECO:0000269|PubMed:20604901}. Note=Enriched on tubular elements of the early endosome membrane. Binds preferentially to highly curved membranes enriched in phosphatidylinositol 3-phosphate, PtdIns(3P or phosphatidylinositol 3,5-bisphosphate, PtdIns(3,5P2, PubMed:15498486. Colocalized with SORT1 to tubular endosomal membrane structures called endosome-to-TGN transport carriers, ETCs which are budding from early endosome vacuoles just before maturing into late endosome vacuoles, PubMed:18088323. Colocalizes with DNAJC13 and Shiginella dysenteria toxin stxB on early endosomes, PubMed:19874558. Colocalized with F-actin at the leading edge of lamellipodia in a KALRN-dependent manner, PubMed:20604901. {ECO:0000269|PubMed:15498486, ECO:0000269|PubMed:18088323, ECO:0000269|PubMed:20604901}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q13596
Gene Ontology
(Biological Process)
Complete annatation
early endosome to Golgi transport [GO:0034498];
intracellular protein transport [GO:0006886];
lamellipodium morphogenesis [GO:0072673];
positive regulation of protein catabolic process [GO:0045732];
receptor internalization [GO:0031623];
retrograde transport, endosome to Golgi [GO:0042147];
vesicle organization [GO:0016050]
Gene Ontology
(Molecular Function)
Complete annatation
epidermal growth factor receptor binding [GO:0005154];
identical protein binding [GO:0042802];
insulin receptor binding [GO:0005158];
leptin receptor binding [GO:1990460];
phosphatidylinositol binding [GO:0035091];
protein heterodimerization activity [GO:0046982];
protein homodimerization activity [GO:0042803];
transferrin receptor binding [GO:1990459]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
early endosome membrane [GO:0031901];
endosome membrane [GO:0010008];
extrinsic component of membrane [GO:0019898];
Golgi apparatus [GO:0005794];
intracellular membrane-bounded organelle [GO:0043231];
lamellipodium [GO:0030027];
membrane [GO:0016020];
protein complex [GO:0043234];
retromer, tubulation complex [GO:0030905];
retromer complex [GO:0030904]
Protein-protein interaction112525
Phylogenetic treeQ13596
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
      Negatively associated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.3699512852020810.2595825073839070.373354406949136
AZA vs. DISU-0.04601422255463490.8553879915937770.989951370113767
AZA vs. IL7-0.03141645515869830.8699033219016250.999311006273513
AZA vs. SAHA0.1279136442721440.5998563631410890.868360205319194
DISU vs. CD30.3113781249453160.3914170144114540.52544483705034
DISU vs. IL70.005315617440788860.9831335633578640.997754944136459
DISU vs. SAHA0.175781006184910.5463971695280450.849556560606331
DMSO vs. AZA0.01322667181560120.9368980431328211
DMSO vs. CD30.372496048497320.2453853614263290.350500660840775
DMSO vs. DISU0.05763654531623280.8129100824482490.979344399543159
DMSO vs. IL7-0.03748830159804320.8344644199105620.963494783989913
DMSO vs. SAHA0.1083108747653520.6458918929443480.886207204211257
HIV vs. Mock in Activation0.06458208787602190.9172952524735720.999983755607037
HIV vs. Mock in Latency0.05810816739843330.7239657282827710.999834320637052
IL7 vs. CD30.3453663365812630.2835536568581440.420049559687745
SAHA vs. CD30.473742054600080.1801998906968860.278774149156439
SAHA vs. IL70.156366776605740.520784036344330.743186086222934
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.602573 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.002 1.043 1.149 1.208 1.178
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
213364_s_at 1.53 No downregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2I4K NMR - A=142-269.
4FZS X-ray 2.8Å A/B=301-522.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04144 Endocytosis - Homo sapiens (human)