Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0007194
UniProt IDP08621
Primary gene name(s)SNRNP70
Synonym gene name(s)RNPU1Z, RPU1, SNRP70, U1AP1
Protein nameU1 small nuclear ribonucleoprotein 70 kDa
Protein functionComponent of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. SNRNP70 binds to the loop I region of U1-snRNA. The truncated isoforms cannot bind U1-snRNA.
Subcellular locationNucleus. Nucleus speckle {ECO:0000250}. Nucleus, nucleoplasm {ECO:0000250}. Note=Colocalizes with SCNM1 and LUC7L2 in nuclear speckles. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P08621
Gene Ontology
(Biological Process)
Complete annatation
cellular response to retinoic acid [GO:0071300];
cellular response to transforming growth factor beta stimulus [GO:0071560];
cellular response to tumor necrosis factor [GO:0071356];
mRNA processing [GO:0006397];
mRNA splicing, via spliceosome [GO:0000398];
negative regulation of chaperone-mediated autophagy [GO:1904715];
negative regulation of protein refolding [GO:0061084];
positive regulation of mRNA splicing, via spliceosome [GO:0048026];
regulation of ATPase activity [GO:0043462];
regulation of RNA splicing [GO:0043484];
RNA splicing [GO:0008380]
Gene Ontology
(Molecular Function)
Complete annatation
mRNA binding [GO:0003729];
nucleotide binding [GO:0000166];
poly(A RNA binding [GO:0044822];
RNA binding [GO:0003723];
U1 snRNA binding [GO:0030619]
Gene Ontology
(Cellular Component)
Complete annatation
commitment complex [GO:0000243];
nuclear speck [GO:0016607];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
precatalytic spliceosome [GO:0071011];
spliceosomal complex [GO:0005681];
U1 snRNP [GO:0005685];
U2-type prespliceosome [GO:0071004]
Protein-protein interaction112509
Phylogenetic treeP08621
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.6953785210584150.04152309734342970.0853917482821903
AZA vs. DISU-0.1752390449908930.4873871557597960.931429104131977
AZA vs. IL7-0.3169139969349610.1139048212869290.866239844226986
AZA vs. SAHA0.0364360144543580.8809493653181120.97377947736344
DISU vs. CD30.5076273155560990.1670917009851060.27751656023022
DISU vs. IL7-0.1512088163300960.5477139169287460.85250972094273
DISU vs. SAHA0.2143449018604920.4613676767573160.799962293650427
DMSO vs. AZA0.06279799096764970.7063642399730241
DMSO vs. CD30.7479528642402470.02822713303306310.0597165790118096
DMSO vs. DISU0.2366441136828440.3310107161926880.832220802973608
DMSO vs. IL7-0.3725059096114060.05477342331244430.460215097633857
DMSO vs. SAHA-0.03214657768064070.891268709009910.973657988857025
HIV vs. Mock in Activation0.3436407919826150.6226869958239870.999983755607037
HIV vs. Mock in Latency0.09292786582354440.5723608201501240.999834320637052
IL7 vs. CD30.3838203017899930.2972815875393990.434788023382628
SAHA vs. CD30.7090949186508570.04942197051910160.0979856723750639
SAHA vs. IL70.3503683481355340.1505730372977790.367390411820447
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.302728 0.038467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.017 1.014 1.01 0.996 0.98
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2L5I NMR - A=131-151.
2L5J NMR - A=131-151.
3CW1 X-ray 5.4Å 6/7/8/K=1-216.
3PGW X-ray 4.4Å L/S=1-437.
4PJO X-ray 3.3Å K/N/k/n=2-60.
4PKD X-ray 2.5Å B=60-215.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Rev interacts with 22174317

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03040 Spliceosome - Homo sapiens (human)
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