Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0007189
UniProt IDQ96H20
Primary gene name(s)SNF8
Synonym gene name(s)EAP30
Protein nameVacuolar-sorting protein SNF8
Protein functionComponent of the endosomal sorting complex required for transport II, ESCRT-II, which is required for multivesicular body, MVB formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL. Required for degradation of both endocytosed EGF and EGFR, but not for the EGFR ligand-mediated internalization. It is also required for the degradation of CXCR4. Required for the exosomal release of SDCBP, CD63 and syndecan, PubMed:22660413. {ECO:0000269|PubMed:17714434, ECO:0000269|PubMed:17959629, ECO:0000269|PubMed:18031739, ECO:0000269|PubMed:22660413}.
Subcellular locationCytoplasm. Endosome membrane. Nucleus {ECO:0000305}. Late endosome membrane. Note=Recruited to the endosome membrane to participate in vesicle formation.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q96H20
Gene Ontology
(Biological Process)
Complete annatation
autophagy [GO:0006914];
early endosome to late endosome transport [GO:0045022];
endocytic recycling [GO:0032456];
endosomal transport [GO:0016197];
multivesicular body assembly [GO:0036258];
multivesicular body sorting pathway [GO:0071985];
positive regulation of exosomal secretion [GO:1903543];
positive regulation of gene expression [GO:0010628];
positive regulation of protein catabolic process [GO:0045732];
protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [GO:0043328];
regulation of multivesicular body size involved in endosome transport [GO:0010797];
regulation of protein catabolic process [GO:0042176];
regulation of protein complex stability [GO:0061635];
regulation of transcription from RNA polymerase II promoter [GO:0006357];
regulation of viral budding via host ESCRT complex [GO:1903772];
transcription, DNA-templated [GO:0006351]
Gene Ontology
(Molecular Function)
Complete annatation
channel regulator activity [GO:0016247];
protein C-terminus binding [GO:0008022];
protein homodimerization activity [GO:0042803];
protein N-terminus binding [GO:0047485];
transcription factor binding [GO:0008134]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
endosome membrane [GO:0010008];
ESCRT II complex [GO:0000814];
extracellular exosome [GO:0070062];
late endosome membrane [GO:0031902];
membrane [GO:0016020];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
perinuclear region of cytoplasm [GO:0048471];
plasma membrane [GO:0005886];
recycling endosome [GO:0055037];
transcription factor complex [GO:0005667]
Protein-protein interaction116425
Phylogenetic treeQ96H20
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.7406972698745860.0244855000924860.055056759271065
AZA vs. DISU-0.2157215149284690.3961306479617760.902635546368717
AZA vs. IL70.06359879743778550.7424042382092140.999311006273513
AZA vs. SAHA-0.08163227968484010.7390345520971370.927345945085511
DISU vs. CD3-0.9689347387165660.008089346541887420.0243137864433617
DISU vs. IL70.2697992599083440.2864159999732840.673161288248878
DISU vs. SAHA0.1362575100018420.6409146436204440.889153447537328
DMSO vs. AZA-0.07746503873110730.6470857964100841
DMSO vs. CD3-0.8301386733194960.009941381251269310.0247835360831329
DMSO vs. DISU0.1362783995514370.5784614223227480.932467317571925
DMSO vs. IL70.1484412918496770.4124381633207950.849123958050597
DMSO vs. SAHA-0.009722119903648760.9672597351675940.992954939630189
HIV vs. Mock in Activation-0.04751845547012310.9393603110593470.999983755607037
HIV vs. Mock in Latency-0.06228266186102580.7083838680853470.999834320637052
IL7 vs. CD3-0.6706825937116250.03767776690331480.0873046857084803
SAHA vs. CD3-0.8465068379399040.01734558076999880.0408498050013881
SAHA vs. IL7-0.147787569270610.5455664719163520.760571261360345
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.223226 0.145528
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.014 0.995 1.092 1.173 1.111
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2ZME X-ray 2.9Å A=1-258.
3CUQ X-ray 2.6Å A=25-258.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
capsid impairs 26268989
Pr55(Gag) regulated by 22978549
HIV-1 virus replication enhanced by expression of human gene 26268989
Pr55(Gag) interacts with 22978549

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04144 Endocytosis - Homo sapiens (human)