Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0007068
UniProt IDQ96B97
Primary gene name(s)SH3KBP1
Synonym gene name(s)CIN85
Protein nameSH3 domain-containing kinase-binding protein 1
Protein functionAdapter protein involved in regulating diverse signal transduction pathways. Involved in the regulation of endocytosis and lysosomal degradation of ligand-induced receptor tyrosine kinases, including EGFR and MET/hepatocyte growth factor receptor, through a association with CBL and endophilins. The association with CBL, and thus the receptor internalization, may inhibited by an interaction with PDCD6IP and/or SPRY2. Involved in regulation of ligand-dependent endocytosis of the IgE receptor. Attenuates phosphatidylinositol 3-kinase activity by interaction with its regulatory subunit, By similarity. May be involved in regulation of cell adhesion; promotes the interaction between TTK2B and PDCD6IP. May be involved in the regulation of cellular stress response via the MAPK pathways through its interaction with MAP3K4. Is involved in modulation of tumor necrosis factor mediated apoptosis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape and migration. {ECO:0000250, ECO:0000269|PubMed:11894095, ECO:0000269|PubMed:11894096, ECO:0000269|PubMed:12177062, ECO:0000269|PubMed:12734385, ECO:0000269|PubMed:12771190, ECO:0000269|PubMed:15090612, ECO:0000269|PubMed:15707590, ECO:0000269|PubMed:16177060, ECO:0000269|PubMed:16256071, ECO:0000269|PubMed:21834987}.
Subcellular locationCytoplasm {ECO:0000269|PubMed:20221403}. Cytoplasm, cytoskeleton. Cytoplasmic vesicle membrane;
Peripheral membrane protein. Cell junction, synapse, synaptosome. Cell junction, focal adhesion {ECO:0000250}. Note=Localized in endocytic vesicles containing clustered receptors. Colocalizes with ASAP1 in vesicular structures. Colocalized with actin microfilaments and focal adhesions, By similarity. Colocalized with MAGI2 in synaptosomes. Translocation to EGFR containing vesicles upon EGF stimulation is inhibited in the presence of SH3KBP1, By similarity. Colocalizes with ZFP36 in the cytoplasm, PubMed:20221403. {ECO:0000250|UniProtKB:Q8R550, ECO:0000269|PubMed:20221403}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q96B97
Gene Ontology
(Biological Process)
Complete annatation
apoptotic process [GO:0006915];
cell-cell signaling [GO:0007267];
cell migration [GO:0016477];
cytoskeleton organization [GO:0007010];
endocytosis [GO:0006897];
negative regulation of epidermal growth factor receptor signaling pathway [GO:0042059];
regulation of cell shape [GO:0008360]
Gene Ontology
(Molecular Function)
Complete annatation
Gene Ontology
(Cellular Component)
Complete annatation
cell-cell junction [GO:0005911];
cytoplasmic vesicle membrane [GO:0030659];
cytoskeleton [GO:0005856];
cytosol [GO:0005829];
endocytic vesicle [GO:0030139];
focal adhesion [GO:0005925];
neuron projection [GO:0043005];
plasma membrane [GO:0005886];
synapse [GO:0045202]
Protein-protein interaction119029
Phylogenetic treeQ96B97
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-2.152256816570234.30580711641149e-073.61947154816252e-06
AZA vs. DISU-0.3217691608297570.2032653722866230.792761247567542
AZA vs. IL7-0.0469674326234540.8067296834090640.999311006273513
AZA vs. SAHA-0.2991210861057110.2206497935676350.589221538112564
DISU vs. CD31.8183162125131.34339549968665e-059.67065127138327e-05
DISU vs. IL70.2655731015805330.2913549258776140.676969599912246
DISU vs. SAHA0.02405087459684620.9341604008928930.984657096486861
DMSO vs. AZA0.01076453521020110.9486454046378891
DMSO vs. CD32.15185524852524.66123759479409e-073.55488417343388e-06
DMSO vs. DISU0.3308408492073250.1749497381227570.68806895994461
DMSO vs. IL7-0.05055532572108710.7782026665937180.95414107268595
DMSO vs. SAHA-0.3163293522642830.1799247216444820.512598389395839
HIV vs. Mock in Activation0.3012103596558650.7419397870541670.999983755607037
HIV vs. Mock in Latency-0.04836373562430210.7693908909490570.999834320637052
IL7 vs. CD32.112452488148736.05746097170901e-076.21116389296393e-06
SAHA vs. CD31.828899182167811.5379550353023e-059.14279383905547e-05
SAHA vs. IL7-0.2553052685946260.2941253181206620.541877908256033
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.517961 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.971 1.2 1.282 1.276 1.629
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2BZ8 X-ray 2.0Å A/B=1-58.
2K6D NMR - A=267-328.
2K9G NMR - A=262-333.
2N64 NMR - A/B/C=594-665.
2O2O NMR - A=92-168.
2YDL X-ray 2.0Å A=270-328.
5ABS X-ray 1.7Å A=599-662.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Nef requires 25496667

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04144 Endocytosis - Homo sapiens (human)