Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0007067
UniProt IDQ9Y371
Primary gene name(s)SH3GLB1
Synonym gene name(s)KIAA0491
Protein nameEndophilin-B1
Protein functionMay be required for normal outer mitochondrial membrane dynamics, PubMed:15452144. Required for coatomer-mediated retrograde transport in certain cells, By similarity. May recruit other proteins to membranes with high curvature. May promote membrane fusion, PubMed:11604418. Involved in activation of caspase-dependent apoptosis by promoting BAX/BAK1 activation, PubMed:16227588. Isoform 1 acts proapoptotic in fibroblasts, By similarity. Involved in caspase-independent apoptosis during nutrition starvation and involved in the regulation of autophagy. Activates lipid kinase activity of PIK3C3 during autophagy probably by associating with the PI3K complex II, PI3KC3-C2, PubMed:17891140. Associated with PI3KC3-C2 during autophagy may regulate the trafficking of ATG9A from the Golgi complex to the peripheral cytoplasm for the formation of autophagosomes by inducing Golgi membrane tubulation and fragmentation, PubMed:21068542. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2, PubMed:20643123. Isoform 2 acts antiapoptotic in neuronal cells; involved in maintenance of mitochondrial morphology and promotes neuronal viability, By similarity. {ECO:0000250|UniProtKB:Q9JK48, ECO:0000269|PubMed:11604418, ECO:0000269|PubMed:15452144, ECO:0000269|PubMed:17891140, ECO:0000269|PubMed:20643123, ECO:0000269|PubMed:21068542}.
Subcellular locationCytoplasm {ECO:0000269|PubMed:15452144}. Golgi apparatus membrane {ECO:0000269|PubMed:21068542};
Peripheral membrane protein {ECO:0000250}. Mitochondrion outer membrane {ECO:0000269|PubMed:15452144};
Peripheral membrane protein {ECO:0000269|PubMed:15452144}. Cytoplasmic vesicle, autophagosome membrane {ECO:0000269|PubMed:17891140}. Midbody {ECO:0000269|PubMed:20643123}. Note=Association with the Golgi apparatus depends on the cell type, By similarity. Following starvation colocalizes with ATG5 and LC3 autophagy-related protein(son autophagosomal membranes, PubMed:17891140. {ECO:0000250, ECO:0000269|PubMed:17891140}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9Y371
Gene Ontology
(Biological Process)
Complete annatation
apoptotic process [GO:0006915];
autophagic cell death [GO:0048102];
autophagy [GO:0006914];
cellular response to amino acid starvation [GO:0034198];
cellular response to glucose starvation [GO:0042149];
membrane fission [GO:0090148];
positive regulation of autophagosome assembly [GO:2000786];
positive regulation of autophagy [GO:0010508];
positive regulation of membrane tubulation [GO:1903527];
positive regulation of protein oligomerization [GO:0032461];
positive regulation of protein targeting to mitochondrion [GO:1903955];
protein localization to vacuolar membrane [GO:1903778];
protein oligomerization [GO:0051259];
receptor catabolic process [GO:0032801];
regulation of cytokinesis [GO:0032465];
regulation of macroautophagy [GO:0016241];
regulation of protein stability [GO:0031647]
Gene Ontology
(Molecular Function)
Complete annatation
identical protein binding [GO:0042802];
lipid binding [GO:0008289];
protein homodimerization activity [GO:0042803]
Gene Ontology
(Cellular Component)
Complete annatation
autophagosome membrane [GO:0000421];
cytoplasm [GO:0005737];
cytoplasmic vesicle [GO:0031410];
extracellular exosome [GO:0070062];
Golgi membrane [GO:0000139];
midbody [GO:0030496];
mitochondrial outer membrane [GO:0005741]
Protein-protein interaction119289
Phylogenetic treeQ9Y371
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.1169702984843280.7208149641736430.802776831536401
AZA vs. DISU0.2197905344103790.3851999219101070.90058897815532
AZA vs. IL70.04355138816863610.8205676381720330.999311006273513
AZA vs. SAHA-0.0464779408004970.8487750577917320.964408468086072
DISU vs. CD30.3244418400977270.3704975342426570.504597021248086
DISU vs. IL7-0.1849157265022840.4629215511959320.803424175026327
DISU vs. SAHA-0.2657989439912170.3630373082941280.737124303158365
DMSO vs. AZA-0.1111730062561240.5064958671205931
DMSO vs. CD3-0.005633169548258080.9859360307924980.991146935868859
DMSO vs. DISU-0.3328450739737710.1730532328832960.684995645850879
DMSO vs. IL70.1619884396375520.3672053121768280.826734340718626
DMSO vs. SAHA0.05717975497546590.8082237121016410.946766717920598
HIV vs. Mock in Activation-0.09916230636737460.8735842769659450.999983755607037
HIV vs. Mock in Latency0.03957117693401370.8102357926002690.999834320637052
IL7 vs. CD30.1695233028321460.5974081881888910.71444477518057
SAHA vs. CD30.04508782671462010.898562470225480.930356332964478
SAHA vs. IL7-0.09439331294778810.6981801187122650.859833914172279
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.327392 0.011166
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.937 1.042 1.08 1.062 1.022
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04144 Endocytosis - Homo sapiens (human)