Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0006966
UniProt IDO43290
Primary gene name(s)SART1
Synonym gene name(s)unknown
Protein nameU4/U6.U5 tri-snRNP-associated protein 1
Protein functionPlays a role in mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the spliceosome. May also bind to DNA. {ECO:0000269|PubMed:11350945}.
Subcellular locationNucleus {ECO:0000269|PubMed:11350945, ECO:0000269|PubMed:9449708}. Note=Found in the nucleus of mitogen-activated peripheral blood mononuclear cells, PBMCs, tumor cells, or normal cell lines, but not in normal tissues except testis and fetal liver or in unstimulated PBMCs, suggesting preferential expression in proliferating cells.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O43290
Gene Ontology
(Biological Process)
Complete annatation
cell cycle arrest [GO:0007050];
intrinsic apoptotic signaling pathway [GO:0097193];
maturation of 5S rRNA [GO:0000481];
mRNA cis splicing, via spliceosome [GO:0045292];
mRNA splicing, via spliceosome [GO:0000398];
positive regulation of cytotoxic T cell differentiation [GO:0045585];
spliceosomal snRNP assembly [GO:0000387]
Gene Ontology
(Molecular Function)
Complete annatation
poly(A RNA binding [GO:0044822]
Gene Ontology
(Cellular Component)
Complete annatation
Cajal body [GO:0015030];
catalytic step 2 spliceosome [GO:0071013];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
Golgi apparatus [GO:0005794];
nucleoplasm [GO:0005654];
U4/U6 x U5 tri-snRNP complex [GO:0046540]
Protein-protein interaction114546
Phylogenetic treeO43290
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.3652089253756570.2656363970378220.380146955326514
AZA vs. DISU-0.00643582695194870.9797210182752230.997994522186162
AZA vs. IL70.03149599450999540.8700254207935480.999311006273513
AZA vs. SAHA-0.179702642519080.4619576820102970.800500068815176
DISU vs. CD3-0.3833627731625790.2929881828654780.423848739780635
DISU vs. IL70.0284151180591850.9101904651457460.982189124739072
DISU vs. SAHA-0.1713185529616970.5565577955595530.854098701632466
DMSO vs. AZA-0.02224012437339890.8946620357983331
DMSO vs. CD3-0.3978318879820190.2157746929187130.317414876361724
DMSO vs. DISU-0.01747887165535310.9428832673393290.992682048260702
DMSO vs. IL70.06099612179904510.7347343354617670.946333083680773
DMSO vs. SAHA-0.1638965202076090.4871761300034130.807104699932004
HIV vs. Mock in Activation0.1618949954424380.7953888541173970.999983755607037
HIV vs. Mock in Latency-0.03679572950438970.8239983050549890.999834320637052
IL7 vs. CD3-0.3273194175086130.3100063273556290.448094109343248
SAHA vs. CD3-0.56872480795840.1096957098475030.187747228552172
SAHA vs. IL7-0.213985887687520.3798714845890330.630497991488117
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0327739 0.881044
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.987 0.99 0.917 0.861 0.992
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4PYU X-ray 2.0Å C/D/H/L/P/T=117-135.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp120 complexes with 23125841
retropepsin cleaves 22944692
Gag-Pol complexes with 23125841
Pr55(Gag) complexes with 23125841
Pr55(Gag) interacts with 26362536
Nef complexes with 23125841

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03040 Spliceosome - Homo sapiens (human)