Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0006914
UniProt IDP08865
Primary gene name(s)RPSA
Synonym gene name(s)LAMBR, LAMR1
Protein name40S ribosomal protein SA
Protein functionRequired for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA. {ECO:0000255|HAMAP-Rule:MF_03016, ECO:0000269|PubMed:16263087, ECO:0000269|PubMed:6300843}.; FUNCTION:, Microbial infection Acts as a receptor for the adeno-associated viruses 2,3,8 and 9, dengue virus, Sindbis virus and Venezuelan equine encephalitis virus, PubMed:1385835, PubMed:15507651, PubMed:16973587. Also acts as a receptor for pathogenic prion protein and bacteria, PubMed:9396609, PubMed:11689427, PubMed:15516338. {ECO:0000269|PubMed:11689427, ECO:0000269|PubMed:1385835, ECO:0000269|PubMed:15507651, ECO:0000269|PubMed:15516338, ECO:0000269|PubMed:16973587, ECO:0000269|PubMed:9396609}.
Subcellular locationCell membrane. Cytoplasm. Nucleus {ECO:0000255|HAMAP-Rule:MF_03016}. Note=67LR is found at the surface of the plasma membrane, with its C-terminal laminin-binding domain accessible to extracellular ligands. 37LRP is found at the cell surface, in the cytoplasm and in the nucleus, By similarity. Colocalizes with PPP1R16B in the cell membrane. {ECO:0000255|HAMAP-Rule:MF_03016}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P08865
Gene Ontology
(Biological Process)
Complete annatation
cell adhesion [GO:0007155];
endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript, SSU-rRNA, 5.8S rRNA, LSU-rRNA [GO:0000447];
endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from, SSU-rRNA, 5.8S rRNA, LSU-rRNA [GO:0000461];
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [GO:0000184];
ribosomal small subunit assembly [GO:0000028];
rRNA export from nucleus [GO:0006407];
rRNA processing [GO:0006364];
SRP-dependent cotranslational protein targeting to membrane [GO:0006614];
translation [GO:0006412];
translational initiation [GO:0006413];
viral transcription [GO:0019083]
Gene Ontology
(Molecular Function)
Complete annatation
laminin receptor activity [GO:0005055];
poly(A RNA binding [GO:0044822];
ribosome binding [GO:0043022];
structural constituent of ribosome [GO:0003735];
virus receptor activity [GO:0001618]
Gene Ontology
(Cellular Component)
Complete annatation
90S preribosome [GO:0030686];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
cytosolic small ribosomal subunit [GO:0022627];
extracellular exosome [GO:0070062];
membrane [GO:0016020];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
plasma membrane [GO:0005886]
Protein-protein interaction110115
Phylogenetic treeP08865
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.1323633800436450.6857546408665470.774093315393994
AZA vs. DISU-0.1448039238613770.5659332048254150.952741360370985
AZA vs. IL70.05869629830311440.7593161736944150.999311006273513
AZA vs. SAHA-0.2439138026734380.3160143922709760.68777171356034
DISU vs. CD3-0.2895564308822940.4247612651409940.556672866521939
DISU vs. IL70.1944730020273270.4399110953444940.790235910667093
DISU vs. SAHA-0.09759993823899280.7370197896933630.923941544707093
DMSO vs. AZA-0.07346952529374560.6590975049499961
DMSO vs. CD3-0.2201873066709180.4907954662545520.599710738837281
DMSO vs. DISU0.0686080785531550.7779280675293720.971585548411322
DMSO vs. IL70.1398679574030230.4350327884012790.860427044280406
DMSO vs. SAHA-0.1760772412872340.4537597410726340.783815353748987
HIV vs. Mock in Activation-0.1902649364026920.7596589529590340.999983755607037
HIV vs. Mock in Latency-0.2139821820841040.1926039125722180.999834320637052
IL7 vs. CD3-0.06578149964427940.8373492650908430.89526886448628
SAHA vs. CD3-0.4007216841766440.2571829607050320.367683067813759
SAHA vs. IL7-0.3059279969730050.2087313368264980.443623268772067
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.639701 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.979 0.901 0.913 0.92 0.835
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB04985 PCK3145 investigational unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3BCH X-ray 2.1Å A=2-220.
4UG0 EM - SA=1-295.
4V6X EM 5.0Å AA=1-295.
5A2Q EM 3.9Å A=1-295.
5AJ0 EM 3.5Å BA=1-295.
5FLX EM 3.9Å A=1-295.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Rev interacts with 22174317

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03010 Ribosome - Homo sapiens (human)