Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0006888
UniProt IDP62269
Primary gene name(s)RPS18
Synonym gene name(s)D6S218E
Protein name40S ribosomal protein S18
Protein functionLocated at the top of the head of the 40S subunit, it contacts several helices of the 18S rRNA. {ECO:0000250}.
Subcellular locationCytoplasm.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P62269
Gene Ontology
(Biological Process)
Complete annatation
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [GO:0000184];
ribosome biogenesis [GO:0042254];
rRNA processing [GO:0006364];
SRP-dependent cotranslational protein targeting to membrane [GO:0006614];
translation [GO:0006412];
translational initiation [GO:0006413];
viral transcription [GO:0019083]
Gene Ontology
(Molecular Function)
Complete annatation
poly(A RNA binding [GO:0044822];
rRNA binding [GO:0019843];
structural constituent of ribosome [GO:0003735]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
cytosolic small ribosomal subunit [GO:0022627];
extracellular exosome [GO:0070062];
focal adhesion [GO:0005925];
membrane [GO:0016020];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
ribosome [GO:0005840];
small ribosomal subunit [GO:0015935]
Protein-protein interaction112136
Phylogenetic treeP62269
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.3762527332956330.2500839586552470.36239452214709
AZA vs. DISU-0.1310424677161170.6035319880809540.955158276076142
AZA vs. IL7-0.05312289433571560.7812881960634140.999311006273513
AZA vs. SAHA-0.07054463487958740.7716677334401030.939817355892067
DISU vs. CD30.2319323583837350.5214809625293680.64340941457912
DISU vs. IL70.06905810361670190.7834250189264350.950465331549835
DISU vs. SAHA0.06163687109762050.8321577243630540.956632270318504
DMSO vs. AZA-0.08989817140987660.5892577059654581
DMSO vs. CD30.2712102121236760.395569649775370.510341421072703
DMSO vs. DISU0.03813209826769380.8754621889185850.987396432719358
DMSO vs. IL70.04450239752335760.8035681283947730.960493870252911
DMSO vs. SAHA0.01309207677557140.9555592358559480.989349060414757
HIV vs. Mock in Activation-0.1980328437401710.7501916630908690.999983755607037
HIV vs. Mock in Latency-0.2318059196967070.1580059624003560.999834320637052
IL7 vs. CD30.3317523790876150.3011384500010470.438938193295211
SAHA vs. CD30.2800656983770550.4272778507741890.541599563560184
SAHA vs. IL7-0.0214224484857390.9296598890073120.97383724845123
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
1.7 1.77E-05 1.2 0.089638533 1.3 0.002724384
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.733219 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.953 0.943 0.881 0.857 0.86
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4UG0 EM - SS=1-152.
4V6X EM 5.0Å AS=1-152.
5A2Q EM 3.9Å S=1-152.
5AJ0 EM 3.5Å BS=1-152.
5FLX EM 3.9Å S=1-152.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03010 Ribosome - Homo sapiens (human)