Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0006813
UniProt IDQ9UBF6
Primary gene name(s)RNF7
Synonym gene name(s)RBX2, ROC2, SAG
Protein nameRING-box protein 2
Protein functionProbable component of the SCF, SKP1-CUL1-F-box protein E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription, PubMed:10851089. CRLs complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins, ARIH1 mediating addition of the first ubiquitin on CRLs targets, By similarity. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F. May play a role in protecting cells from apoptosis induced by redox agents. {ECO:0000250|UniProtKB:P62877, ECO:0000269|PubMed:10851089}.
Subcellular locationCytoplasm {ECO:0000269|PubMed:10082581}. Nucleus {ECO:0000269|PubMed:10082581}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9UBF6
Gene Ontology
(Biological Process)
Complete annatation
protein neddylation [GO:0045116];
protein ubiquitination involved in ubiquitin-dependent protein catabolic process [GO:0042787];
response to redox state [GO:0051775];
SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [GO:0031146]
Gene Ontology
(Molecular Function)
Complete annatation
copper ion binding [GO:0005507];
cullin family protein binding [GO:0097602];
NEDD8 transferase activity [GO:0019788];
ubiquitin protein ligase activity [GO:0061630];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
Cul2-RING ubiquitin ligase complex [GO:0031462];
Cul3-RING ubiquitin ligase complex [GO:0031463];
Cul4-RING E3 ubiquitin ligase complex [GO:0080008];
Cul5-RING ubiquitin ligase complex [GO:0031466];
Cul7-RING ubiquitin ligase complex [GO:0031467];
cytoplasm [GO:0005737];
nuclear SCF ubiquitin ligase complex [GO:0043224];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction114977
Phylogenetic treeQ9UBF6
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.01848982619045430.9550028763970280.970847542682354
AZA vs. DISU-0.04728193789404520.85211896296620.989489441774853
AZA vs. IL7-0.1010430473203830.6014704207652770.999311006273513
AZA vs. SAHA-0.1759687228972790.4726395215272110.805931508933019
DISU vs. CD3-0.04196157437821290.9078401531273250.939848372319647
DISU vs. IL7-0.06288753841097760.8034074622827860.957093747475326
DISU vs. SAHA-0.1266917354293810.664322734912890.897853183854195
DMSO vs. AZA-0.02324207350216620.8905694181008431
DMSO vs. CD3-0.01763082676296270.9560631603248750.96918723573745
DMSO vs. DISU0.02187760716819150.9287563699468440.991032960072147
DMSO vs. IL7-0.07042790253479580.6971738913444960.937788846060647
DMSO vs. SAHA-0.1583587401819280.5036517691339690.816801443303352
HIV vs. Mock in Activation-0.1747363869281750.7792335714358860.999983755607037
HIV vs. Mock in Latency0.001564260067689170.9924841756918690.999834320637052
IL7 vs. CD3-0.07536508600689830.8146702089458790.879748105705777
SAHA vs. CD3-0.1817624689264690.6072602897908980.703738278002832
SAHA vs. IL7-0.07765488777289080.7507940174362370.887862460642147
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.23948 0.079345
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.9 0.924 0.905 0.996 1.06
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2ECL NMR - A=40-113.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vif interacts with 22190034
Vif complexes with 22190037

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04120 Ubiquitin mediated proteolysis - Homo sapiens (human)