Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0006812
UniProt IDQ99942
Primary gene name(s)RNF5
Synonym gene name(s)G16, NG2, RMA1
Protein nameE3 ubiquitin-protein ligase RNF5
Protein functionHas E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin and Salmonella type III secreted protein sopA. May be involved in regulation of cell motility and localization of PXN/paxillin. Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD; the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N. Mediates the 'Lys-48'-linked polyubiquitination of TMEM173 at 'Lys-150' leading to its proteasomal degradation; the ubiquitination occurrs in mitochondria after viral transfection and regulates antiviral responses. {ECO:0000269|PubMed:11329381, ECO:0000269|PubMed:12861019, ECO:0000269|PubMed:16176924, ECO:0000269|PubMed:19269966, ECO:0000269|PubMed:19285439}.
Subcellular locationMembrane;
Multi-pass membrane protein. Mitochondrion membrane. Endoplasmic reticulum membrane. Note=Predominantly located in the plasma membrane, with some localization occurring within cytoplasmic organelles.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q99942
Gene Ontology
(Biological Process)
Complete annatation
cellular protein catabolic process [GO:0044257];
ERAD pathway [GO:0036503];
ER-associated misfolded protein catabolic process [GO:0071712];
negative regulation of autophagy [GO:0010507];
protein destabilization [GO:0031648];
protein K48-linked ubiquitination [GO:0070936];
protein K63-linked ubiquitination [GO:0070534];
protein ubiquitination involved in ubiquitin-dependent protein catabolic process [GO:0042787];
regulation of autophagosome assembly [GO:2000785];
response to bacterium [GO:0009617]
Gene Ontology
(Molecular Function)
Complete annatation
ligase activity [GO:0016874];
ubiquitin protein ligase activity involved in ERAD pathway [GO:1904264];
ubiquitin-protein transferase activity [GO:0004842];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
endoplasmic reticulum membrane [GO:0005789];
integral component of membrane [GO:0016021];
mitochondrial membrane [GO:0031966]
Protein-protein interaction111975
Phylogenetic treeQ99942
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      Yes - Two siRNA pools inhibit HIV replication and inhibition of Tat-mediated transactivation of the HIV LTR is not observed
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.03858443635595630.9063056079625070.939530787757817
AZA vs. DISU0.112367344683160.6578515337521150.960426582104957
AZA vs. IL7-0.09605307880964040.6229437057572560.999311006273513
AZA vs. SAHA0.05999251114617180.8064927819400820.950662511336003
DISU vs. CD30.1387391744275210.7019008319688170.791285793855811
DISU vs. IL7-0.2186816229064730.386854689555760.755125527004154
DISU vs. SAHA-0.04944152935267090.8659056591468140.963879424258703
DMSO vs. AZA0.08954539191780480.5956987732435091
DMSO vs. CD30.1166774160222160.7155451845432090.792701792626113
DMSO vs. DISU-0.02470713992482980.9195809792785130.988899823982894
DMSO vs. IL7-0.1783843240569540.3240121018020440.800221869993146
DMSO vs. SAHA-0.03432526296781120.8846034782566680.971193040903484
HIV vs. Mock in Activation0.1117662634658860.8577415640083860.999983755607037
HIV vs. Mock in Latency0.05122814382513690.7577049386807080.999834320637052
IL7 vs. CD3-0.05286202609560320.8694161931704080.916733842578353
SAHA vs. CD30.07564054036554480.8310339767351520.880709003473891
SAHA vs. IL70.1544426777157860.5277181999582710.749182910733751
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.255854 0.0712676
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.915 1.043 0.757 0.863 0.952
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp160; precursor interacts with 22190034
HIV-1 virus replication regulated by expression of human gene 18976975

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04141 Protein processing in endoplasmic reticulum - Homo sapiens (human)