Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0006811
UniProt IDQ9H4P4
Primary gene name(s)RNF41
Synonym gene name(s)FLRF, NRDP1
Protein nameE3 ubiquitin-protein ligase NRDP1
Protein functionActs as E3 ubiquitin-protein ligase and regulates the degradation of target proteins. Polyubiquitinates MYD88. Negatively regulates MYD88-dependent production of proinflammatory cytokines. Can promote TRIF-dependent production of type I interferon and inhibits infection with vesicular stomatitis virus, By similarity. Promotes also activation of TBK1 and IRF3. Involved in the ubiquitination of erythropoietin, EPO and interleukin-3, IL-3 receptors. Thus, through maintaining basal levels of cytokine receptors, RNF41 is involved in the control of hematopoietic progenitor cell differentiation into myeloerythroid lineages, By similarity. Contributes to the maintenance of steady-state ERBB3 levels by mediating its growth factor-independent degradation. Involved in the degradation of the inhibitor of apoptosis BIRC6 and thus is an important regulator of cell death by promoting apoptosis. Acts also as a PARK2 modifier that accelerates its degradation, resulting in a reduction of PARK2 activity, influencing the balance of intracellular redox state. The RNF41-PARK2 pathway regulates autophagosome-lysosome fusion during late mitophagy. Mitophagy is a selective form of autophagy necessary for mitochondrial quality control, PubMed:24949970. {ECO:0000250, ECO:0000250|UniProtKB:Q8BH75, ECO:0000269|PubMed:12411582, ECO:0000269|PubMed:14765125, ECO:0000269|PubMed:15632191, ECO:0000269|PubMed:17210635, ECO:0000269|PubMed:18541373, ECO:0000269|PubMed:19483718, ECO:0000269|PubMed:24949970}.
Subcellular locationunknown
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9H4P4
Gene Ontology
(Biological Process)
Complete annatation
autophagy [GO:0006914];
ERBB2 signaling pathway [GO:0038128];
extrinsic apoptotic signaling pathway [GO:0097191];
negative regulation of cell migration [GO:0030336];
negative regulation of cell proliferation [GO:0008285];
negative regulation of macromitophagy [GO:1901525];
positive regulation of reactive oxygen species metabolic process [GO:2000379];
positive regulation of sequence-specific DNA binding transcription factor activity [GO:0051091];
proteasomal protein catabolic process [GO:0010498];
protein autoubiquitination [GO:0051865];
protein polyubiquitination [GO:0000209];
regulation of establishment of cell polarity [GO:2000114];
regulation of lymphocyte differentiation [GO:0045619];
regulation of MAPK cascade [GO:0043408];
regulation of myeloid cell differentiation [GO:0045637];
regulation of protein kinase B signaling [GO:0051896];
regulation of reactive oxygen species metabolic process [GO:2000377]
Gene Ontology
(Molecular Function)
Complete annatation
acid-amino acid ligase activity [GO:0016881];
protein tag [GO:0031386];
Ral GTPase binding [GO:0017160];
ubiquitin protein ligase activity [GO:0061630];
ubiquitin-protein transferase activity [GO:0004842];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829]
Protein-protein interaction115488
Phylogenetic treeQ9H4P4
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.405109525729990.2169531853879860.324718809351314
AZA vs. DISU0.01497434378741970.9529206499120120.996649735504266
AZA vs. IL70.02085450132772540.914142333672320.999311006273513
AZA vs. SAHA0.1746417543848270.4768746321355040.807867858486243
DISU vs. CD3-0.402407038738740.2675124306229040.395574100373715
DISU vs. IL7-0.003690730659601520.9883385878893150.998873975089261
DISU vs. SAHA0.161573643862230.580596961247980.865147684815138
DMSO vs. AZA0.02731283724625570.8715944523650271
DMSO vs. CD3-0.3886920558640530.2250081497693740.328368475857368
DMSO vs. DISU0.01053943823992540.9656349626615780.994306419554359
DMSO vs. IL70.000781828940283420.9965521493047740.999784441946487
DMSO vs. SAHA0.1412235423060290.5517157558009960.838941200974091
HIV vs. Mock in Activation0.2054079305535110.7413884725363090.999983755607037
HIV vs. Mock in Latency0.01691912104615660.918844302278840.999834320637052
IL7 vs. CD3-0.3778339988894440.2403899752717160.3703758184681
SAHA vs. CD3-0.2543472450650210.4727881455186120.583881989813383
SAHA vs. IL70.1512072758409420.5372514984600190.754696813119977
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.190729 0.179674
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.728 1.021 0.986 0.765 0.814
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2FZP X-ray 1.8Å A=193-317.
2GWF X-ray 2.3Å B/D/F=193-317.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04144 Endocytosis - Homo sapiens (human)