Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0006722
UniProt IDQ06330
Primary gene name(s)RBPJ
Synonym gene name(s)IGKJRB, IGKJRB1, RBPJK, RBPSUH
Protein nameRecombining binding protein suppressor of hairless
Protein functionTranscriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins, Notch intracellular domain, it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. Binds specifically to methylated DNA. Binds to the oxygen responsive element of COX4I2 and activates its transcription under hypoxia conditions, 4% oxygen, PubMed:23303788. {ECO:0000269|PubMed:21991380, ECO:0000269|PubMed:23303788}.
Subcellular locationNucleus. Cytoplasm. Note=Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm, down-regulating the Notch signaling pathway.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q06330
Gene Ontology
(Biological Process)
Complete annatation
angiogenesis [GO:0001525];
arterial endothelial cell fate commitment [GO:0060844];
atrioventricular canal development [GO:0036302];
auditory receptor cell fate commitment [GO:0009912];
B cell differentiation [GO:0030183];
blood vessel endothelial cell fate specification [GO:0097101];
blood vessel lumenization [GO:0072554];
blood vessel remodeling [GO:0001974];
cardiac left ventricle morphogenesis [GO:0003214];
Clara cell differentiation [GO:0060486];
defense response to bacterium [GO:0042742];
DNA recombination [GO:0006310];
dorsal aorta morphogenesis [GO:0035912];
endocardium morphogenesis [GO:0003160];
epidermal cell fate specification [GO:0009957];
epithelial to mesenchymal transition [GO:0001837];
epithelial to mesenchymal transition involved in endocardial cushion formation [GO:0003198];
hair follicle maturation [GO:0048820];
humoral immune response [GO:0006959];
inflammatory response to antigenic stimulus [GO:0002437];
interleukin-4 secretion [GO:0072602];
keratinocyte differentiation [GO:0030216];
labyrinthine layer blood vessel development [GO:0060716];
myeloid dendritic cell differentiation [GO:0043011];
negative regulation of cell differentiation [GO:0045596];
negative regulation of cell proliferation [GO:0008285];
negative regulation of ossification [GO:0030279];
negative regulation of transcription, DNA-templated [GO:0045892];
negative regulation of transcription from RNA polymerase II promoter [GO:0000122];
Notch signaling involved in heart development [GO:0061314];
Notch signaling pathway [GO:0007219];
outflow tract morphogenesis [GO:0003151];
pituitary gland development [GO:0021983];
positive regulation of BMP signaling pathway [GO:0030513];
positive regulation of canonical Wnt signaling pathway involved in cardiac muscle cell fate commitment [GO:1901297];
positive regulation of cardiac muscle cell proliferation [GO:0060045];
positive regulation of cell proliferation involved in heart morphogenesis [GO:2000138];
positive regulation of ephrin receptor signaling pathway [GO:1901189];
positive regulation of ERBB signaling pathway [GO:1901186];
positive regulation of transcription from RNA polymerase II promoter [GO:0045944];
positive regulation of transcription from RNA polymerase II promoter in response to hypoxia [GO:0061419];
positive regulation of transcription of Notch receptor target [GO:0007221];
regulation of timing of cell differentiation [GO:0048505];
regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation [GO:0003256];
sebaceous gland development [GO:0048733];
secondary heart field specification [GO:0003139];
somatic stem cell population maintenance [GO:0035019];
somitogenesis [GO:0001756];
transcription initiation from RNA polymerase II promoter [GO:0006367];
ventricular trabecula myocardium morphogenesis [GO:0003222]
Gene Ontology
(Molecular Function)
Complete annatation
chromatin binding [GO:0003682];
DNA binding [GO:0003677];
recombinase activity [GO:0000150];
RNA polymerase II core promoter proximal region sequence-specific DNA binding [GO:0000978];
RNA polymerase II core promoter sequence-specific DNA binding [GO:0000979];
RNA polymerase II repressing transcription factor binding [GO:0001103];
sequence-specific DNA binding [GO:0043565];
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding [GO:0001077];
transcription factor activity, sequence-specific DNA binding [GO:0003700];
transcription factor binding [GO:0008134]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
MAML1-RBP-Jkappa- ICN1 complex [GO:0002193];
nucleolus [GO:0005730];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
transcription factor complex [GO:0005667]
Protein-protein interaction109736
Phylogenetic treeQ06330
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD33.2375474521348400
AZA vs. DISU0.1730175430077250.4942781705416620.934114849489241
AZA vs. IL70.03224659493916870.8665585034146180.999311006273513
AZA vs. SAHA-0.1529638656431250.5300865578915950.83775684353721
DISU vs. CD3-3.07697203428057.43849426498855e-153.87985683565527e-13
DISU vs. IL7-0.1505866609046550.5499402455536450.853135097518281
DISU vs. SAHA-0.3238677581505480.2671240664457330.644854525933629
DMSO vs. AZA-0.06031009103547210.727610555069931
DMSO vs. CD3-3.3050491293004100
DMSO vs. DISU-0.2338294135230190.3388772231060080.83759035206982
DMSO vs. IL70.0990618931344580.5811410926478220.907460167501459
DMSO vs. SAHA-0.09934390273686660.6730406153701470.897786781843686
HIV vs. Mock in Activation0.3714126410214850.5509796809686060.999983755607037
HIV vs. Mock in Latency0.712764560129021.64583979082034e-050.00239765922684388
IL7 vs. CD3-3.1997431909417800
SAHA vs. CD3-3.4135274497798700
SAHA vs. IL7-0.1871831674007130.4415849766612940.681807661165787
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.329282 0.0175948
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.955 1.041 0.936 0.864 1.024
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2F8X X-ray 3.2Å C=23-449.
3NBN X-ray 3.4Å A/D=23-448.
3V79 X-ray 3.8Å C=23-449.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04330 Notch signaling pathway - Homo sapiens (human)
hsa04658 Th1 and Th2 cell differentiation - Homo sapiens (human)
hsa05169 Epstein-Barr virus infection - Homo sapiens (human)
hsa05203 Viral carcinogenesis - Homo sapiens (human)