Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0006695
UniProt IDP28749
Primary gene name(s)RBL1
Synonym gene name(s)unknown
Protein nameRetinoblastoma-like protein 1
Protein functionKey regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation. Forms a complex with adenovirus E1A and with SV40 large T antigen. May bind and modulate functionally certain cellular proteins with which T and E1A compete for pocket binding. May act as a tumor suppressor.
Subcellular locationNucleus {ECO:0000305}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P28749
Gene Ontology
(Biological Process)
Complete annatation
cell cycle [GO:0007049];
covalent chromatin modification [GO:0016569];
negative regulation of gene expression [GO:0010629];
negative regulation of transcription from RNA polymerase II promoter [GO:0000122];
positive regulation of transcription from RNA polymerase II promoter [GO:0045944];
regulation of cell cycle [GO:0051726];
regulation of lipid kinase activity [GO:0043550];
transcription, DNA-templated [GO:0006351];
viral process [GO:0016032]
Gene Ontology
(Molecular Function)
Complete annatation
transcription factor binding [GO:0008134]
Gene Ontology
(Cellular Component)
Complete annatation
nucleoplasm [GO:0005654];
transcription factor complex [GO:0005667]
Protein-protein interaction111868
Phylogenetic treeP28749
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.6946225113026350.2127015744471640.319816253997173
AZA vs. DISU0.02120806590335880.9334381555153650.995042856396371
AZA vs. IL70.3178078423021640.1013505976277730.84139376197593
AZA vs. SAHA-0.04244056560829560.8624059760035920.967606121153661
DISU vs. CD3-0.6861232853769740.2079520212058250.328512761229994
DISU vs. IL70.2879019584397220.2545288691126330.640785354354198
DISU vs. SAHA-0.06283467234248710.8298657381789560.955780348695759
DMSO vs. AZA-0.01674612591219320.9210900886064061
DMSO vs. CD3-0.7230911574977580.1938120327546150.291773585834744
DMSO vs. DISU-0.03985029185602430.8707615621531840.985829520069753
DMSO vs. IL70.3417297129255840.05974929913498950.47154745593772
DMSO vs. SAHA-0.03281962037966550.8896769039136880.972815832466313
HIV vs. Mock in Activation0.002266605132982290.9984877969050690.999983755607037
HIV vs. Mock in Latency0.08052770480455250.6282591700799520.999834320637052
IL7 vs. CD3-0.3681370192833750.5093018878574120.637865172885315
SAHA vs. CD3-0.7626835690490130.1814794295890860.280243365499286
SAHA vs. IL7-0.3643275793883680.1378574867572630.348844007953497
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.145673 0.363945
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.882 0.98 0.753 0.672 0.79
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1H28 X-ray 2.8Å E/F=653-663.
4YOO X-ray 2.4Å A=391-600# A=781-972.
4YOS X-ray 2.3Å A=391-599.
4YOZ X-ray 2.2Å A=391-593# A=777-972.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04110 Cell cycle - Homo sapiens (human)
hsa04350 TGF-beta signaling pathway - Homo sapiens (human)
hsa05203 Viral carcinogenesis - Homo sapiens (human)