Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0006626
UniProt IDQ15286
Primary gene name(s)RAB35
Synonym gene name(s)RAB1C, RAY
Protein nameRas-related protein Rab-35
Protein functionThe small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in the process of endocytosis and is an essential rate-limiting regulator of the fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate, PIP2 and SEPT2 localization at the intercellular bridge. May indirectly regulate neurite outgrowth. {ECO:0000269|PubMed:16950109, ECO:0000269|PubMed:21951725}.
Subcellular locationCell membrane {ECO:0000269|PubMed:16950109, ECO:0000269|PubMed:21951725};
Lipid-anchor {ECO:0000305};
Cytoplasmic side {ECO:0000305}. Membrane, clathrin-coated pit {ECO:0000269|PubMed:16950109}. Cytoplasmic vesicle, clathrin-coated vesicle {ECO:0000269|PubMed:16950109}. Endosome {ECO:0000269|PubMed:16950109, ECO:0000269|PubMed:21951725}. Melanosome {ECO:0000269|PubMed:17081065}. Note=Present on sorting endosomes and recycling endosome tubules, PubMed:16950109. Tends to be enriched in PIP2-positive cell membrane domains, PubMed:16950109. During mitosis, associated with the plasma membrane and present at the ingressing furrow during early cytokinesis as well as at the intercellular bridge later during cytokinesis, PubMed:16950109. Identified in stage I to stage IV melanosomes, PubMed:17081065. {ECO:0000269|PubMed:16950109, ECO:0000269|PubMed:17081065}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q15286
Gene Ontology
(Biological Process)
Complete annatation
antigen processing and presentation [GO:0019882];
cellular response to nerve growth factor stimulus [GO:1990090];
cytokinesis [GO:0000910];
endocytic recycling [GO:0032456];
endosomal transport [GO:0016197];
ER to Golgi vesicle-mediated transport [GO:0006888];
neuron projection development [GO:0031175];
plasma membrane to endosome transport [GO:0048227];
protein localization [GO:0008104];
protein localization to endosome [GO:0036010];
protein transport [GO:0015031];
small GTPase mediated signal transduction [GO:0007264]
Gene Ontology
(Molecular Function)
Complete annatation
GDP binding [GO:0019003];
GTPase activity [GO:0003924];
GTP binding [GO:0005525];
phosphatidylinositol-4,5-bisphosphate binding [GO:0005546]
Gene Ontology
(Cellular Component)
Complete annatation
cell projection membrane [GO:0031253];
clathrin-coated endocytic vesicle [GO:0045334];
coated pit [GO:0005905];
endosome membrane [GO:0010008];
extracellular exosome [GO:0070062];
intercellular bridge [GO:0045171];
melanosome [GO:0042470];
mitochondrion [GO:0005739];
plasma membrane [GO:0005886]
Protein-protein interaction116211
Phylogenetic treeQ15286
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.437469358048760.1824180324581270.283970727660493
AZA vs. DISU0.2849269856999250.2606285925428660.840546727426436
AZA vs. IL7-0.03910619282061780.8390867598528360.999311006273513
AZA vs. SAHA-0.3701411077389890.1301377211609420.459521201400806
DISU vs. CD3-0.1657544842287670.6471150535289830.748972075928739
DISU vs. IL7-0.3336141500026260.1862022653088360.559386040161558
DISU vs. SAHA-0.6522737136165920.025822819386690.188789391287472
DMSO vs. AZA0.06841502145697830.6837229303554621
DMSO vs. CD3-0.3806469242263850.2347934238864730.338938571373105
DMSO vs. DISU-0.2184947780701350.3710966200519910.851890501597821
DMSO vs. IL7-0.1001421552537390.5782049588022960.905672503991634
DMSO vs. SAHA-0.4439587115589880.06043608248006730.282064435858811
HIV vs. Mock in Activation0.127236988327610.8381577901199070.999983755607037
HIV vs. Mock in Latency-0.01042553235950050.9497472276857640.999834320637052
IL7 vs. CD3-0.4707702540386930.1439489075068010.250953259640297
SAHA vs. CD3-0.8309911670966670.01943502633604670.0449694222146819
SAHA vs. IL7-0.3330412051440370.1723886615070520.397300649211305
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.102921 0.520329
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.04 1.01 0.89 0.871 1.045
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3TW8 X-ray 2.1Å B/D=1-180.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
HIV-1 virus replication enhanced by expression of human gene 18976975

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04144 Endocytosis - Homo sapiens (human)