Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0006549
UniProt IDP55036
Primary gene name(s)PSMD4
Synonym gene name(s)MCB1
Protein name26S proteasome non-ATPase regulatory subunit 4
Protein functionBinds and presumably selects ubiquitin-conjugates for destruction. Displays selectivity for longer polyubiquitin chains. Modulates intestinal fluid secretion.
Subcellular locationunknown
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P55036
Gene Ontology
(Biological Process)
Complete annatation
anaphase-promoting complex-dependent catabolic process [GO:0031145];
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent [GO:0002479];
Fc-epsilon receptor signaling pathway [GO:0038095];
MAPK cascade [GO:0000165];
negative regulation of canonical Wnt signaling pathway [GO:0090090];
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [GO:0051436];
NIK/NF-kappaB signaling [GO:0038061];
positive regulation of canonical Wnt signaling pathway [GO:0090263];
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition [GO:0051437];
proteasome assembly [GO:0043248];
proteasome-mediated ubiquitin-dependent protein catabolic process [GO:0043161];
protein polyubiquitination [GO:0000209];
regulation of cellular amino acid metabolic process [GO:0006521];
regulation of mRNA stability [GO:0043488];
stimulatory C-type lectin receptor signaling pathway [GO:0002223];
T cell receptor signaling pathway [GO:0050852];
tumor necrosis factor-mediated signaling pathway [GO:0033209];
Wnt signaling pathway, planar cell polarity pathway [GO:0060071]
Gene Ontology
(Molecular Function)
Complete annatation
identical protein binding [GO:0042802];
poly(A RNA binding [GO:0044822];
polyubiquitin binding [GO:0031593]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
proteasome accessory complex [GO:0022624];
proteasome complex [GO:0000502];
proteasome regulatory particle, base subcomplex [GO:0008540]
Protein-protein interaction111683
Phylogenetic treeP55036
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      Yes - Two siRNA pools inhibit HIV replication and inhibition of Tat-mediated transactivation of the HIV LTR is also observed
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.076551779000070.001182785432790160.00420943141511529
AZA vs. DISU0.1136215616950510.6542647701410810.960057478314726
AZA vs. IL70.1052294764516030.5840336669904650.999311006273513
AZA vs. SAHA-0.1167498961126480.6324171567791510.884163997522623
DISU vs. CD3-0.9763461895614280.008435979763881220.0251212878231421
DISU vs. IL7-0.01778753635287270.9438712797660170.989971442459661
DISU vs. SAHA-0.2279434633309350.4342835671293510.7814874048501
DMSO vs. AZA0.002080213681366860.9900932605606411
DMSO vs. CD3-1.08650461618430.0008044905798259010.00282772995452104
DMSO vs. DISU-0.1136123436495850.6424504070940080.9472626202031
DMSO vs. IL70.1104269884412470.5390003636809550.896696737481162
DMSO vs. SAHA-0.1246944001742240.5970483390786640.863542756070494
HIV vs. Mock in Activation-0.03640383955711170.9533838934229180.999983755607037
HIV vs. Mock in Latency0.004817198499767710.9767299649189330.999834320637052
IL7 vs. CD3-0.9645417316172760.002976034198253870.0108143113477051
SAHA vs. CD3-1.217187662594510.0007440788142034640.00277762391133173
SAHA vs. IL7-0.2246125241194820.3568236667589340.607357718238953
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.274456 0.0370456
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.044 1.195 1.134 1.026 1.048
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1P9C NMR - A=263-307.
1P9D NMR - S=263-307.
1UEL NMR - B=263-307.
1YX4 NMR - A=196-306.
1YX5 NMR - A=192-306.
1YX6 NMR - A=196-306.
2KDE NMR - A=196-306.
2KDF NMR - A=196-306.
5GJQ EM 4.5Å W=1-377.
5GJR EM 3.5Å AA/W=1-377.
5L4K EM 4.5Å W=1-377.
5T0C EM 3.8Å Ab/Bb=1-377.
5T0G EM 4.4Å b=1-377.
5T0H EM 6.8Å b=1-377.
5T0I EM 8.0Å b=1-377.
5T0J EM 8.0Å b=1-377.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vpr interacts with 20614012
Tat enhances 9079628
Tat inhibits 14550573
Vif interacts with 9811770
integrase degraded by 10893419
HIV-1 virus replication enhanced by expression of human gene 18976975
Tat interacts with 12419264

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03050 Proteasome - Homo sapiens (human)
hsa05169 Epstein-Barr virus infection - Homo sapiens (human)