Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0006547
UniProt IDQ13200
Primary gene name(s)PSMD2
Synonym gene name(s)TRAP2
Protein name26S proteasome non-ATPase regulatory subunit 2
Protein functionActs as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.; FUNCTION: Binds to the intracellular domain of tumor necrosis factor type 1 receptor. The binding domain of TRAP1 and TRAP2 resides outside the death domain of TNFR1.
Subcellular locationunknown
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q13200
Gene Ontology
(Biological Process)
Complete annatation
anaphase-promoting complex-dependent catabolic process [GO:0031145];
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent [GO:0002479];
Fc-epsilon receptor signaling pathway [GO:0038095];
MAPK cascade [GO:0000165];
negative regulation of canonical Wnt signaling pathway [GO:0090090];
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [GO:0051436];
NIK/NF-kappaB signaling [GO:0038061];
positive regulation of canonical Wnt signaling pathway [GO:0090263];
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition [GO:0051437];
proteasome-mediated ubiquitin-dependent protein catabolic process [GO:0043161];
protein polyubiquitination [GO:0000209];
regulation of cellular amino acid metabolic process [GO:0006521];
regulation of mRNA stability [GO:0043488];
stimulatory C-type lectin receptor signaling pathway [GO:0002223];
T cell receptor signaling pathway [GO:0050852];
tumor necrosis factor-mediated signaling pathway [GO:0033209];
Wnt signaling pathway, planar cell polarity pathway [GO:0060071]
Gene Ontology
(Molecular Function)
Complete annatation
enzyme regulator activity [GO:0030234]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
extracellular exosome [GO:0070062];
membrane [GO:0016020];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
proteasome accessory complex [GO:0022624];
proteasome complex [GO:0000502];
proteasome regulatory particle [GO:0005838];
proteasome regulatory particle, base subcomplex [GO:0008540];
proteasome storage granule [GO:0034515]
Protein-protein interaction111681
Phylogenetic treeQ13200
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.146892736664250.0005415759008760010.00213234900156821
AZA vs. DISU0.6504841228228220.01039252120886290.251482972870201
AZA vs. IL70.1619988019395480.3984290339382770.999311006273513
AZA vs. SAHA-0.1832993682930620.4519746608327020.792954613290521
DISU vs. CD3-0.5100042864496790.1622776403526160.271736630823743
DISU vs. IL7-0.4977475445364660.04851882463196070.290592483641957
DISU vs. SAHA-0.8315091301270110.004525677317148920.0601330428074916
DMSO vs. AZA0.03078789835762190.8537950590305861
DMSO vs. CD3-1.128108998352720.0004887421135354450.00182838339717196
DMSO vs. DISU-0.6218935224559610.01108271041995930.224926907397096
DMSO vs. IL70.138634034254140.4396544924051780.862319993886034
DMSO vs. SAHA-0.2201876444353960.3499933489421150.700671031273806
HIV vs. Mock in Activation0.04920550833191860.9368612857615070.999983755607037
HIV vs. Mock in Latency-0.1034411414263880.5303551849263450.999834320637052
IL7 vs. CD3-0.9779670152388420.00255097138080840.00946597528875553
SAHA vs. CD3-1.354358393928870.0001703386236013850.000765812368040832
SAHA vs. IL7-0.3481836141967920.1526817086831920.37025805609563
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.260536 0.0508139
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.001 1.085 1.069 1.056 1.034
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
5GJQ EM 4.5Å Z=1-908.
5GJR EM 3.5Å AC/Z=1-908.
5L4K EM 4.5Å Z=1-908.
5T0C EM 3.8Å Af/Bf=1-908.
5T0G EM 4.4Å f=164-908.
5T0H EM 6.8Å f=164-908.
5T0I EM 8.0Å f=164-908.
5T0J EM 8.0Å f=164-908.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vpr interacts with 20614012
Tat enhances 9079628
Envelope surface glycoprotein gp120 complexes with 23125841
Tat inhibits 14550573
Gag-Pol complexes with 23125841
Vif interacts with 9811770
Pr55(Gag) complexes with 23125841
Tat upregulates 23166591
integrase degraded by 10893419
Tat interacts with 12419264
Nef complexes with 23125841

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03050 Proteasome - Homo sapiens (human)
hsa05169 Epstein-Barr virus infection - Homo sapiens (human)