Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0006543
UniProt IDP62195
Primary gene name(s)PSMC5
Synonym gene name(s)SUG1
Protein name26S protease regulatory subunit 8
Protein functionThe 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory, or ATPase complex confers ATP dependency and substrate specificity to the 26S complex.
Subcellular locationCytoplasm {ECO:0000305}. Nucleus {ECO:0000305}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P62195
Gene Ontology
(Biological Process)
Complete annatation
anaphase-promoting complex-dependent catabolic process [GO:0031145];
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent [GO:0002479];
ER-associated ubiquitin-dependent protein catabolic process [GO:0030433];
Fc-epsilon receptor signaling pathway [GO:0038095];
MAPK cascade [GO:0000165];
negative regulation of canonical Wnt signaling pathway [GO:0090090];
negative regulation of programmed cell death [GO:0043069];
negative regulation of transcription, DNA-templated [GO:0045892];
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [GO:0051436];
NIK/NF-kappaB signaling [GO:0038061];
positive regulation of canonical Wnt signaling pathway [GO:0090263];
positive regulation of inclusion body assembly [GO:0090261];
positive regulation of RNA polymerase II transcriptional preinitiation complex assembly [GO:0045899];
positive regulation of transcription, DNA-templated [GO:0045893];
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition [GO:0051437];
proteasome-mediated ubiquitin-dependent protein catabolic process [GO:0043161];
protein polyubiquitination [GO:0000209];
regulation of cellular amino acid metabolic process [GO:0006521];
regulation of mRNA stability [GO:0043488];
stimulatory C-type lectin receptor signaling pathway [GO:0002223];
T cell receptor signaling pathway [GO:0050852];
transcription from RNA polymerase II promoter [GO:0006366];
tumor necrosis factor-mediated signaling pathway [GO:0033209];
Wnt signaling pathway, planar cell polarity pathway [GO:0060071]
Gene Ontology
(Molecular Function)
Complete annatation
ATPase activity [GO:0016887];
ATP binding [GO:0005524];
proteasome-activating ATPase activity [GO:0036402];
TBP-class protein binding [GO:0017025];
thyrotropin-releasing hormone receptor binding [GO:0031531];
transcription cofactor activity [GO:0003712];
transcription factor binding [GO:0008134]
Gene Ontology
(Cellular Component)
Complete annatation
blood microparticle [GO:0072562];
cytoplasm [GO:0005737];
cytoplasmic vesicle [GO:0031410];
cytosol [GO:0005829];
cytosolic proteasome complex [GO:0031597];
extracellular exosome [GO:0070062];
inclusion body [GO:0016234];
membrane [GO:0016020];
nuclear proteasome complex [GO:0031595];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
proteasome accessory complex [GO:0022624];
proteasome complex [GO:0000502];
proteasome regulatory particle, base subcomplex [GO:0008540]
Protein-protein interaction111678
Phylogenetic treeP62195
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.88438972666490.007338081107850060.0200470470287323
AZA vs. DISU0.1433326757898840.5724845828630440.954304256505839
AZA vs. IL70.2309198227950450.2294937464836920.989557594204189
AZA vs. SAHA-0.2454527563221210.3142938492738990.686021645519078
DISU vs. CD3-0.7544438714046120.04024176244656340.0906597410132263
DISU vs. IL70.07826696598358620.7567610840420630.941786906610111
DISU vs. SAHA-0.386233586052090.1871920880487960.553641365621198
DMSO vs. AZA0.02496791885352360.8814541650863531
DMSO vs. CD3-0.8704687569102430.006868001392515090.0180129597803342
DMSO vs. DISU-0.1202049033179080.623616651249430.944350198173819
DMSO vs. IL70.2132431703235780.2353557477881340.742256882407138
DMSO vs. SAHA-0.2763821608312990.2410515531933060.589416914849288
HIV vs. Mock in Activation0.0005312634906217620.9993183062144990.999983755607037
HIV vs. Mock in Latency-0.08461129454860950.6081191605767330.999834320637052
IL7 vs. CD3-0.6468741141625380.04484917511188270.100164708321441
SAHA vs. CD3-1.153541658677710.001265577744428930.0043827701464422
SAHA vs. IL7-0.4788134520451950.04956079444950220.182931384567385
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.156492 0.501976
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.996 1.02 1.064 1.045 0.928
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2KRK NMR - A=320-395.
3KW6 X-ray 2.1Å A=318-395.
5GJQ EM 4.5Å J=1-406.
5GJR EM 3.5Å J/x=1-406.
5L4G EM 4.0Å J=1-406.
5T0C EM 3.8Å AC/BC=1-406.
5T0G EM 4.4Å C=9-406.
5T0H EM 6.8Å C=9-406.
5T0I EM 8.0Å C=9-406.
5T0J EM 8.0Å C=9-406.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat enhances 9079628
Tat inhibits 14550573
Vif interacts with 9811770
integrase degraded by 10893419
HIV-1 virus replication enhanced by expression of human gene 18854154
matrix downregulates 26469385
Tat interacts with 12419264

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03050 Proteasome - Homo sapiens (human)
hsa05169 Epstein-Barr virus infection - Homo sapiens (human)