Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0006542
UniProt IDP43686
Primary gene name(s)PSMC4
Synonym gene name(s)MIP224, TBP7
Protein name26S protease regulatory subunit 6B
Protein functionThe 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory, or ATPase complex confers ATP dependency and substrate specificity to the 26S complex. {ECO:0000269|PubMed:8060531}.
Subcellular locationCytoplasm. Nucleus.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P43686
Gene Ontology
(Biological Process)
Complete annatation
anaphase-promoting complex-dependent catabolic process [GO:0031145];
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent [GO:0002479];
blastocyst development [GO:0001824];
ER-associated ubiquitin-dependent protein catabolic process [GO:0030433];
Fc-epsilon receptor signaling pathway [GO:0038095];
MAPK cascade [GO:0000165];
negative regulation of canonical Wnt signaling pathway [GO:0090090];
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [GO:0051436];
NIK/NF-kappaB signaling [GO:0038061];
positive regulation of canonical Wnt signaling pathway [GO:0090263];
positive regulation of RNA polymerase II transcriptional preinitiation complex assembly [GO:0045899];
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition [GO:0051437];
proteasome-mediated ubiquitin-dependent protein catabolic process [GO:0043161];
protein polyubiquitination [GO:0000209];
proteolysis [GO:0006508];
regulation of cellular amino acid metabolic process [GO:0006521];
regulation of mRNA stability [GO:0043488];
stimulatory C-type lectin receptor signaling pathway [GO:0002223];
T cell receptor signaling pathway [GO:0050852];
tumor necrosis factor-mediated signaling pathway [GO:0033209];
Wnt signaling pathway, planar cell polarity pathway [GO:0060071]
Gene Ontology
(Molecular Function)
Complete annatation
ATPase activity [GO:0016887];
ATP binding [GO:0005524];
proteasome-activating ATPase activity [GO:0036402];
TBP-class protein binding [GO:0017025]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
cytosolic proteasome complex [GO:0031597];
inclusion body [GO:0016234];
membrane [GO:0016020];
nuclear proteasome complex [GO:0031595];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
proteasome accessory complex [GO:0022624];
proteasome complex [GO:0000502];
proteasome regulatory particle, base subcomplex [GO:0008540]
Protein-protein interaction111677
Phylogenetic treeP43686
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.364890015495444.14297899480331e-050.000221507553776785
AZA vs. DISU0.530449669175230.0365560963714610.442963502173153
AZA vs. IL70.06983301876319340.7172011765866460.999311006273513
AZA vs. SAHA0.0903996059431960.7118289874020940.918972149307812
DISU vs. CD3-0.8481241402243510.01997221792580450.0513931739524713
DISU vs. IL7-0.4699757348004240.06288393903159860.330535348989313
DISU vs. SAHA-0.4372480194495440.1343921148803050.470919219567794
DMSO vs. AZA-0.00208405480976440.9901187843104081
DMSO vs. CD3-1.378195066938812.27486069241944e-050.000120206160404416
DMSO vs. DISU-0.5343929548498830.02912586486090860.344375004071223
DMSO vs. IL70.07926982497419090.660150966788460.93033334807292
DMSO vs. SAHA0.08677137022292850.7142531909191010.914649489365432
HIV vs. Mock in Activation0.0120197794171140.9845863674648690.999983755607037
HIV vs. Mock in Latency-0.04022153294427540.8084125115904550.999834320637052
IL7 vs. CD3-1.288881761290937.7535836956133e-050.000467292151315391
SAHA vs. CD3-1.298130688397620.0003023800903194650.00126415740305238
SAHA vs. IL70.01807093941612160.9411399034488020.977311325505629
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.218373 0.132151
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.986 1.046 1.061 1.04 0.963
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
201252_at 1.46 No upregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2DVW X-ray 2.3Å B=337-418.
5GJQ EM 4.5Å K=1-418.
5GJR EM 3.5Å K/y=1-418.
5L4G EM 4.0Å K=1-418.
5T0C EM 3.8Å AD/BD=1-418.
5T0G EM 4.4Å D=1-418.
5T0H EM 6.8Å D=1-418.
5T0I EM 8.0Å D=1-418.
5T0J EM 8.0Å D=1-418.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat enhances 9079628
Tat inhibits 14550573
9079628
12419264
14550573
retropepsin cleaves 22944692
Vif interacts with 9811770
9846577
12167863
12719574
12750511
12808465
12808466
12809610
12830140
12840737
12859895
12914693
12920286
12970355
14527406
14528300
14528301
14557625
14564014
14614829
integrase degraded by 10893419
HIV-1 virus replication enhanced by expression of human gene 18854154
Tat interacts with 12419264
2194290
8323548
8419915
8500623
9714759
11463857

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03050 Proteasome - Homo sapiens (human)
hsa05169 Epstein-Barr virus infection - Homo sapiens (human)
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