Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0006524
UniProt IDQ8NDX1
Primary gene name(s)PSD4
Synonym gene name(s)EFA6B, TIC
Protein namePH and SEC7 domain-containing protein 4
Protein functionGuanine nucleotide exchange factor for ARF6 and ARL14/ARF7. Through ARL14 activation, controls the movement of MHC class II-containing vesicles along the actin cytoskeleton in dendritic cells. Involved in membrane recycling. Interacts with several phosphatidylinositol phosphate species, including phosphatidylinositol 3,4-bisphosphate, phosphatidylinositol 3,5-bisphosphate and phosphatidylinositol 4,5-bisphosphate. {ECO:0000269|PubMed:12082148, ECO:0000269|PubMed:21458045}.
Subcellular locationCell membrane {ECO:0000269|PubMed:12082148}. Note=Accumulates in dynamic actin-rich membrane ruffles and microvilli-like structures. Recruited to membranes via phosphatidylinositol phosphate-binding.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q8NDX1
Gene Ontology
(Biological Process)
Complete annatation
regulation of ARF protein signal transduction [GO:0032012]
Gene Ontology
(Molecular Function)
Complete annatation
ARF guanyl-nucleotide exchange factor activity [GO:0005086];
phospholipid binding [GO:0005543]
Gene Ontology
(Cellular Component)
Complete annatation
membrane [GO:0016020];
plasma membrane [GO:0005886]
Protein-protein interaction117094
Phylogenetic treeQ8NDX1
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.052297121550410.001597082901828810.00546316576620214
AZA vs. DISU-0.4554242558156540.1118544028734350.661836042652969
AZA vs. IL7-0.01873260617144730.9221311926245140.999311006273513
AZA vs. SAHA-0.4003898776709690.1006857015068440.400951749838685
DISU vs. CD30.5854644751780750.1145107568543730.207507979021711
DISU vs. IL70.4268636340872120.1147907956753370.443844044547145
DISU vs. SAHA0.05787829224500130.8433235759495820.958718383732077
DMSO vs. AZA0.05816528700974880.7272774441797751
DMSO vs. CD31.100600845625360.0007105355755224750.00253654465527158
DMSO vs. DISU0.5122537195269440.05151544460279860.445067187377236
DMSO vs. IL7-0.06975154097815740.6970025407727680.937788846060647
DMSO vs. SAHA-0.4637474554210550.04910416556082010.249726106403629
HIV vs. Mock in Activation0.2393369853182790.7002509887856770.999983755607037
HIV vs. Mock in Latency0.1286243699956660.437075053163190.999834320637052
IL7 vs. CD31.038578983580010.001423964131404220.00576044319717011
SAHA vs. CD30.6295285298127770.07665576181226560.140655342811499
SAHA vs. IL7-0.3834166849986330.114945467152750.312292017103145
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.0146745 0.948416
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.992 0.992 1.115 1.076 1.159
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04144 Endocytosis - Homo sapiens (human)