Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0006514
UniProt IDO94906
Primary gene name(s)PRPF6
Synonym gene name(s)C20orf14
Protein namePre-mRNA-processing factor 6
Protein functionInvolved in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex, one of the building blocks of the spliceosome. Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but does not affect estrogen-induced transactivation. {ECO:0000269|PubMed:12039962}.
Subcellular locationNucleus, nucleoplasm. Nucleus speckle. Note=Localized in splicing speckles.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O94906
Gene Ontology
(Biological Process)
Complete annatation
mRNA splicing, via spliceosome [GO:0000398];
positive regulation of transcription from RNA polymerase II promoter [GO:0045944];
RNA localization [GO:0006403];
RNA splicing [GO:0008380];
RNA splicing, via transesterification reactions [GO:0000375];
spliceosomal complex assembly [GO:0000245];
spliceosomal tri-snRNP complex assembly [GO:0000244]
Gene Ontology
(Molecular Function)
Complete annatation
androgen receptor binding [GO:0050681];
poly(A RNA binding [GO:0044822];
ribonucleoprotein complex binding [GO:0043021];
RNA binding [GO:0003723];
transcription coactivator activity [GO:0003713]
Gene Ontology
(Cellular Component)
Complete annatation
catalytic step 2 spliceosome [GO:0071013];
membrane [GO:0016020];
nuclear speck [GO:0016607];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
spliceosomal complex [GO:0005681];
U4/U6 x U5 tri-snRNP complex [GO:0046540];
U5 snRNP [GO:0005682]
Protein-protein interaction117298
Phylogenetic treeO94906
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.4500560703099440.169826862876770.268671195263847
AZA vs. DISU0.1487423095287640.5561767038510540.948689448488927
AZA vs. IL7-0.01994605234397840.9172945951254820.999311006273513
AZA vs. SAHA-0.03571912941998980.8835004863835450.974727906886092
DISU vs. CD30.5871532169556190.1064074642487790.195737840734214
DISU vs. IL7-0.1782994276951480.4786678657935880.81294126650647
DISU vs. SAHA-0.1825909074437750.5308962298586770.842223612674329
DMSO vs. AZA-0.0136966562021860.9347963912493491
DMSO vs. CD30.425664324286060.1839292628118370.279777637319578
DMSO vs. DISU-0.1641945329952910.5005180726652790.90822484708799
DMSO vs. IL70.001052692928555510.9953246019074380.999784441946487
DMSO vs. SAHA-0.02824986233485860.9046073995617120.977516936535183
HIV vs. Mock in Activation0.1073349122054590.8631696619326880.999983755607037
HIV vs. Mock in Latency0.04663227191839810.777330363754790.999834320637052
IL7 vs. CD30.4363944783039720.1750027419919940.291675064889726
SAHA vs. CD30.3904842777581610.269143493506320.381130656562699
SAHA vs. IL7-0.01866657179920980.9388728729736030.976524942082016
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.0855915 0.619914
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.02 1.006 1.018 1.004 1.025
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3JCR EM 7.0Å G=1-941.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Rev interacts with 22174317
HIV-1 virus replication enhanced by expression of human gene 18854154

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03040 Spliceosome - Homo sapiens (human)
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