Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0006492
UniProt IDP22694
Primary gene name(s)PRKACB
Synonym gene name(s)unknown
Protein namecAMP-dependent protein kinase catalytic subunit beta
Protein functionMediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. {ECO:0000269|PubMed:12420224, ECO:0000269|PubMed:21423175}.
Subcellular locationCytoplasm {ECO:0000269|PubMed:21423175}. Cell membrane {ECO:0000269|PubMed:21423175}. Membrane {ECO:0000305};
Lipid-anchor {ECO:0000305}. Nucleus {ECO:0000250}. Note=Translocates into the nucleus, monomeric catalytic subunit. The inactive holoenzyme is found in the cytoplasm. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P22694
Gene Ontology
(Biological Process)
Complete annatation
activation of protein kinase A activity [GO:0034199];
adenylate cyclase-modulating G-protein coupled receptor signaling pathway [GO:0007188];
blood coagulation [GO:0007596];
cellular response to glucagon stimulus [GO:0071377];
lipoprotein metabolic process [GO:0042157];
negative regulation of meiotic cell cycle [GO:0051447];
negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterning [GO:1901621];
neural tube closure [GO:0001843];
protein phosphorylation [GO:0006468];
regulation of protein processing [GO:0070613];
renal water homeostasis [GO:0003091];
response to clozapine [GO:0097338];
signal transduction [GO:0007165];
stimulatory C-type lectin receptor signaling pathway [GO:0002223]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
cAMP-dependent protein kinase activity [GO:0004691];
kinase activity [GO:0016301];
magnesium ion binding [GO:0000287];
protein serine/threonine kinase activity [GO:0004674];
ubiquitin protein ligase binding [GO:0031625]
Gene Ontology
(Cellular Component)
Complete annatation
cAMP-dependent protein kinase complex [GO:0005952];
centrosome [GO:0005813];
ciliary base [GO:0097546];
cytosol [GO:0005829];
extracellular exosome [GO:0070062];
nucleoplasm [GO:0005654];
perinuclear region of cytoplasm [GO:0048471];
plasma membrane [GO:0005886]
Protein-protein interaction111554
Phylogenetic treeP22694
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-2.186389984380352.23284754019559e-082.4475256818784e-07
AZA vs. DISU-0.2502035010699390.3222650909177730.871827706477839
AZA vs. IL7-0.08101730604043880.6726282167645480.999311006273513
AZA vs. SAHA-0.4543332703976820.06409618636499870.310358282633391
DISU vs. CD31.924602284624213.75832443055835e-074.00657584626849e-06
DISU vs. IL70.1602395046197620.523849860887660.83937655760354
DISU vs. SAHA-0.2042457701431420.4835434529087050.813880207592929
DMSO vs. AZA-0.1778995466246430.2863773420145381
DMSO vs. CD31.998177598004436.95067980949204e-086.29278794050467e-07
DMSO vs. DISU0.07059074419393750.7720793369290220.97150326349898
DMSO vs. IL70.1039974230574950.5619781588923630.901842672763832
DMSO vs. SAHA-0.2844801050524410.229737553280130.576102273791154
HIV vs. Mock in Activation-0.1743836169788440.8352934070300450.999983755607037
HIV vs. Mock in Latency0.02855802007696540.8623995548409970.999834320637052
IL7 vs. CD32.114682490412087.32183570439915e-089.24305105296553e-07
SAHA vs. CD31.706611965700172.16822802234784e-050.000124619360399626
SAHA vs. IL7-0.3778449069623330.1213437962764750.322883739641939
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.600223 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.96 1.163 1.459 1.51 1.048
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB02482 Phosphonothreonine experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
matrix phosphorylated by 9151826
Tat inhibits 20433920
Envelope surface glycoprotein gp120 interacts with 12972513
22114277
Tat activates 9278385
9671211
9730685
Nef phosphorylated by 15629779
19895210
Envelope surface glycoprotein gp120 inhibited by 19100722
capsid phosphorylated by 12842892
21651489
Vif inhibited by 18836454
24189052
Tat upregulates 21745501
Vpr phosphorylated by 20392842
21651489
Envelope surface glycoprotein gp120 inactivates 9540970
matrix activates 1978848
7688126
794609020402410

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa01522 Endocrine resistance - Homo sapiens (human)
hsa04010 MAPK signaling pathway - Homo sapiens (human)
hsa04014 Ras signaling pathway - Homo sapiens (human)
hsa04020 Calcium signaling pathway - Homo sapiens (human)
hsa04024 cAMP signaling pathway - Homo sapiens (human)
hsa04062 Chemokine signaling pathway - Homo sapiens (human)
hsa04114 Oocyte meiosis - Homo sapiens (human)
hsa04211 Longevity regulating pathway - Homo sapiens (human)
hsa04213 Longevity regulating pathway - multiple species - Homo sapiens (human)
hsa04261 Adrenergic signaling in cardiomyocytes - Homo sapiens (human)
hsa04270 Vascular smooth muscle contraction - Homo sapiens (human)
hsa04310 Wnt signaling pathway - Homo sapiens (human)
hsa04340 Hedgehog signaling pathway - Homo sapiens (human)
hsa04530 Tight junction - Homo sapiens (human)
hsa04540 Gap junction - Homo sapiens (human)
hsa04611 Platelet activation - Homo sapiens (human)
hsa04713 Circadian entrainment - Homo sapiens (human)
hsa04720 Long-term potentiation - Homo sapiens (human)
hsa04723 Retrograde endocannabinoid signaling - Homo sapiens (human)
hsa04724 Glutamatergic synapse - Homo sapiens (human)
hsa04725 Cholinergic synapse - Homo sapiens (human)
hsa04726 Serotonergic synapse - Homo sapiens (human)
hsa04727 GABAergic synapse - Homo sapiens (human)
hsa04728 Dopaminergic synapse - Homo sapiens (human)
hsa04740 Olfactory transduction - Homo sapiens (human)
hsa04742 Taste transduction - Homo sapiens (human)
hsa04750 Inflammatory mediator regulation of TRP channels - Homo sapiens (human)
hsa04910 Insulin signaling pathway - Homo sapiens (human)
hsa04911 Insulin secretion - Homo sapiens (human)
hsa04912 GnRH signaling pathway - Homo sapiens (human)
hsa04913 Ovarian steroidogenesis - Homo sapiens (human)
hsa04914 Progesterone-mediated oocyte maturation - Homo sapiens (human)
hsa04915 Estrogen signaling pathway - Homo sapiens (human)
hsa04916 Melanogenesis - Homo sapiens (human)
hsa04918 Thyroid hormone synthesis - Homo sapiens (human)
hsa04919 Thyroid hormone signaling pathway - Homo sapiens (human)
hsa04921 Oxytocin signaling pathway - Homo sapiens (human)
hsa04922 Glucagon signaling pathway - Homo sapiens (human)
hsa04923 Regulation of lipolysis in adipocytes - Homo sapiens (human)
hsa04924 Renin secretion - Homo sapiens (human)
hsa04925 Aldosterone synthesis and secretion - Homo sapiens (human)
hsa04961 Endocrine and other factor-regulated calcium reabsorption - Homo sapiens (human)
hsa04962 Vasopressin-regulated water reabsorption - Homo sapiens (human)
hsa04970 Salivary secretion - Homo sapiens (human)
hsa04971 Gastric acid secretion - Homo sapiens (human)
hsa04976 Bile secretion - Homo sapiens (human)
hsa05012 Parkinson's disease - Homo sapiens (human)
hsa05020 Prion diseases - Homo sapiens (human)
hsa05030 Cocaine addiction - Homo sapiens (human)
hsa05031 Amphetamine addiction - Homo sapiens (human)
hsa05032 Morphine addiction - Homo sapiens (human)
hsa05110 Vibrio cholerae infection - Homo sapiens (human)
hsa05146 Amoebiasis - Homo sapiens (human)
hsa05166 HTLV-I infection - Homo sapiens (human)
hsa05169 Epstein-Barr virus infection - Homo sapiens (human)
hsa05200 Pathways in cancer - Homo sapiens (human)
hsa05203 Viral carcinogenesis - Homo sapiens (human)
hsa05205 Proteoglycans in cancer - Homo sapiens (human)
hsa05414 Dilated cardiomyopathy - Homo sapiens (human)
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