Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0006485
UniProt IDQ9HCU5
Primary gene name(s)PREB
Synonym gene name(s)SEC12
Protein nameProlactin regulatory element-binding protein
Protein functionGuanine nucleotide exchange factor that specifically activates the small GTPase SAR1B. Mediates the recruitement of SAR1B and other COPII coat components to endoplasmic reticulum membranes and is therefore required for the formation of COPII transport vesicles from the ER. {ECO:0000250|UniProtKB:Q9WTV0, ECO:0000250|UniProtKB:Q9WUQ2}.; FUNCTION: Was first identified based on its probable role in the regulation of pituitary gene transcription. Binds to the prolactin gene, PRL promoter and seems to activate transcription. {ECO:0000250|UniProtKB:Q9WTV0}.
Subcellular locationEndoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q9WTV0};
Single-pass membrane protein {ECO:0000250|UniProtKB:Q9WTV0}. Nucleus {ECO:0000250|UniProtKB:Q9WTV0}. Note=Concentrates at endoplasmic reticulum exit sites. {ECO:0000269|PubMed:25202031}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9HCU5
Gene Ontology
(Biological Process)
Complete annatation
COPII vesicle coating [GO:0048208];
ER to Golgi vesicle-mediated transport [GO:0006888];
IRE1-mediated unfolded protein response [GO:0036498];
protein exit from endoplasmic reticulum [GO:0032527];
protein secretion [GO:0009306];
regulation of COPII vesicle coating [GO:0003400];
regulation of transcription, DNA-templated [GO:0006355];
transcription, DNA-templated [GO:0006351]
Gene Ontology
(Molecular Function)
Complete annatation
DNA binding [GO:0003677];
guanyl-nucleotide exchange factor activity [GO:0005085]
Gene Ontology
(Cellular Component)
Complete annatation
endoplasmic reticulum membrane [GO:0005789];
Golgi membrane [GO:0000139];
integral component of membrane [GO:0016021];
membrane [GO:0016020];
nucleus [GO:0005634]
Protein-protein interaction115419
Phylogenetic treeQ9HCU5
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.3872673065628940.2373027051473590.348157925823128
AZA vs. DISU-0.1710862300796710.4993267476339330.934754900374469
AZA vs. IL70.004468548679787970.9814967680187750.999311006273513
AZA vs. SAHA-0.0492846350482350.8400434077483020.962210367024559
DISU vs. CD3-0.570297040699810.1164565856833430.210260698597523
DISU vs. IL70.1660597288221360.5104665311978920.832295614942863
DISU vs. SAHA0.1239441403238490.6708097313348350.900006539744262
DMSO vs. AZA0.06248250986265880.7099947572779141
DMSO vs. CD3-0.3376367101345280.2912065755729840.401007958044515
DMSO vs. DISU0.2314098118843630.3435862405630030.840447423589398
DMSO vs. IL7-0.05049066170096750.7792993071566090.954496023349518
DMSO vs. SAHA-0.1168253655819320.620491766075410.87560708505294
HIV vs. Mock in Activation0.07164479099695280.9084176092538070.999983755607037
HIV vs. Mock in Latency-0.006750135583248050.9674491262254110.999834320637052
IL7 vs. CD3-0.3766353368561530.2414457914794720.371510046444475
SAHA vs. CD3-0.4597223502436430.1937877029415950.294749766181856
SAHA vs. IL7-0.05614903076856420.8177676392567630.921292110729731
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.422897 0.225631
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.021 1.119 1.244 1.332 1.064
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vpu interacts with 22190034

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04141 Protein processing in endoplasmic reticulum - Homo sapiens (human)