Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0006477
UniProt IDO60828
Primary gene name(s)PQBP1
Synonym gene name(s)NPW38
Protein namePolyglutamine-binding protein 1
Protein functionProbably functions as scaffold protein that is part of numerous complexes and thereby plays a role in pre-mRNA splicing, transcription regulation and neuron development. Required for normal alternative splicing of target pre-mRNA species, PubMed:23512658. May suppress the ability of POU3F2 to transactivate the DRD1 gene in a POU3F2 dependent manner. Can activate transcription directly or via association with the transcription machinery. May be involved in ATXN1 mutant-induced cell death. The interaction with ATXN1 mutant reduces levels of phosphorylated RNA polymerase II large subunit. {ECO:0000269|PubMed:10198427, ECO:0000269|PubMed:10332029, ECO:0000269|PubMed:12062018, ECO:0000269|PubMed:20410308, ECO:0000269|PubMed:23512658}.
Subcellular locationNucleus {ECO:0000269|PubMed:10198427, ECO:0000269|PubMed:10332029, ECO:0000269|PubMed:12062018, ECO:0000269|PubMed:23512658}. Nucleus speckle {ECO:0000250}. Note=Colocalizes with SRSF2 in nuclear speckles, By similarity. Colocalized with POU3F2. Colocalized with ATXN1 in nuclear inclusion bodies. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O60828
Gene Ontology
(Biological Process)
Complete annatation
alternative mRNA splicing, via spliceosome [GO:0000380];
neuron projection development [GO:0031175];
regulation of dendrite morphogenesis [GO:0048814];
regulation of RNA splicing [GO:0043484];
regulation of transcription, DNA-templated [GO:0006355];
transcription, DNA-templated [GO:0006351]
Gene Ontology
(Molecular Function)
Complete annatation
DNA binding [GO:0003677];
ribonucleoprotein complex binding [GO:0043021];
transcription coactivator activity [GO:0003713]
Gene Ontology
(Cellular Component)
Complete annatation
centrosome [GO:0005813];
ciliary base [GO:0097546];
cytoplasm [GO:0005737];
cytoplasmic stress granule [GO:0010494];
neuronal ribonucleoprotein granule [GO:0071598];
nuclear speck [GO:0016607];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
primary cilium [GO:0072372]
Protein-protein interaction115393
Phylogenetic treeO60828
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.4267200768608240.1946268521465410.298931612712999
AZA vs. DISU0.2797152385064430.2716435644556530.845621663457437
AZA vs. IL7-0.01100901282054470.9545981391676260.999311006273513
AZA vs. SAHA-0.2697578878592870.2710794619832950.645639407479252
DISU vs. CD3-0.1593921075338860.6640576383086560.761970376788056
DISU vs. IL7-0.3001426764904540.2354625758604010.62039392556596
DISU vs. SAHA-0.5470893162982740.06303660524970640.317799046742155
DMSO vs. AZA-0.0256062038970220.8795080809476671
DMSO vs. CD3-0.4630068422511640.1504126291250690.23759738468797
DMSO vs. DISU-0.3070509751370710.2102778305762920.723490161366375
DMSO vs. IL70.02191625268881040.9035486914354230.979523619218739
DMSO vs. SAHA-0.2500689323470020.291369978924990.642945100941571
HIV vs. Mock in Activation0.1082453874968060.8622402837633710.999983755607037
HIV vs. Mock in Latency0.04919722464918110.767330419243240.999834320637052
IL7 vs. CD3-0.4316912772487940.1821103178957530.300487008390004
SAHA vs. CD3-0.7201853569467960.04277071508784090.0872080471348617
SAHA vs. IL7-0.2610113883895410.2863775930301750.533558133749764
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.387187 0.0164862
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.983 1.037 0.868 0.839 0.979
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4BWQ X-ray 2.1Å B/D/F/H=223-265.
4BWS X-ray 2.5Å B/E=229-265.
4CDO X-ray 2.5Å A/C=223-265.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03040 Spliceosome - Homo sapiens (human)