Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0006468
UniProt IDP63098
Primary gene name(s)PPP3R1
Synonym gene name(s)CNA2, CNB
Protein nameCalcineurin subunit B type 1
Protein functionRegulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.
Subcellular locationCytoplasm, cytosol {ECO:0000250}. Cell membrane {ECO:0000250}. Cell membrane, sarcolemma. Membrane {ECO:0000305};
Lipid-anchor {ECO:0000305}. Note=Translocates from the cytosol to the sarcolemma in a CIB1-dependent manner during cardiomyocytes hypertrophy. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P63098
Gene Ontology
(Biological Process)
Complete annatation
calcineurin-NFAT signaling cascade [GO:0033173];
Fc-epsilon receptor signaling pathway [GO:0038095];
positive regulation of NFAT protein import into nucleus [GO:0051533];
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway [GO:1900740];
positive regulation of transcription from RNA polymerase II promoter [GO:0045944];
Wnt signaling pathway, calcium modulating pathway [GO:0007223]
Gene Ontology
(Molecular Function)
Complete annatation
calcium-dependent protein serine/threonine phosphatase activity [GO:0004723];
calcium ion binding [GO:0005509];
calmodulin binding [GO:0005516];
protein domain specific binding [GO:0019904]
Gene Ontology
(Cellular Component)
Complete annatation
calcineurin complex [GO:0005955];
cytosol [GO:0005829];
mitochondrion [GO:0005739];
nucleoplasm [GO:0005654];
sarcolemma [GO:0042383]
Protein-protein interaction111526
Phylogenetic treeP63098
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.009858910305010.002258966033635850.00735041797473218
AZA vs. DISU0.2864397276585210.2607486012941940.840698033700523
AZA vs. IL70.1177846225673280.5412282595834890.999311006273513
AZA vs. SAHA0.1132188501329850.6431258434564740.889611842976484
DISU vs. CD3-0.7354562274643290.04372383220583440.0968115520961733
DISU vs. IL7-0.177588457112790.4829803561487030.816205281945407
DISU vs. SAHA-0.1724207272760720.5576291521436450.854762912119292
DMSO vs. AZA-0.1222719426018280.4676108465866721
DMSO vs. CD3-1.141229594051140.0004247276232652860.00161578829406352
DMSO vs. DISU-0.4099204201784760.09564778861305860.561569786929451
DMSO vs. IL70.2469638316981590.1711846718017190.681879201707603
DMSO vs. SAHA0.2277537992808950.3349853204304970.689389785838446
HIV vs. Mock in Activation-0.03827513439816620.9509036336221010.999983755607037
HIV vs. Mock in Latency0.04720545709148670.775668206798110.999834320637052
IL7 vs. CD3-0.8838285937014430.006287621429391430.0203332584864229
SAHA vs. CD3-0.9217968847001720.009760511339416470.0251265640391196
SAHA vs. IL7-0.008021345891446170.9737721063976560.990461587843337
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0927374 0.56929
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.943 1.141 1.475 1.592 1.134
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB04906 ISA247 investigational unknown unknown
DB08231 MYRISTIC ACID experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1AUI X-ray 2.1Å B=2-170.
1M63 X-ray 2.8Å B/F=2-170.
1MF8 X-ray 3.1Å B=1-170.
2P6B X-ray 2.3Å B/D=16-170.
3LL8 X-ray 2.0Å B/D=16-170.
4F0Z X-ray 1.7Å B=1-170.
4OR9 X-ray 2.2Å B=1-170.
4ORA X-ray 2.7Å B=1-170.
4ORC X-ray 2.7Å B=1-170.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat activates 12482669
Tat upregulates 22632162
Envelope surface glycoprotein gp120 regulated by 24086760

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04010 MAPK signaling pathway - Homo sapiens (human)
hsa04020 Calcium signaling pathway - Homo sapiens (human)
hsa04022 cGMP-PKG signaling pathway - Homo sapiens (human)
hsa04114 Oocyte meiosis - Homo sapiens (human)
hsa04310 Wnt signaling pathway - Homo sapiens (human)
hsa04360 Axon guidance - Homo sapiens (human)
hsa04370 VEGF signaling pathway - Homo sapiens (human)
hsa04380 Osteoclast differentiation - Homo sapiens (human)
hsa04650 Natural killer cell mediated cytotoxicity - Homo sapiens (human)
hsa04658 Th1 and Th2 cell differentiation - Homo sapiens (human)
hsa04659 Th17 cell differentiation - Homo sapiens (human)
hsa04660 T cell receptor signaling pathway - Homo sapiens (human)
hsa04662 B cell receptor signaling pathway - Homo sapiens (human)
hsa04720 Long-term potentiation - Homo sapiens (human)
hsa04724 Glutamatergic synapse - Homo sapiens (human)
hsa04921 Oxytocin signaling pathway - Homo sapiens (human)
hsa04922 Glucagon signaling pathway - Homo sapiens (human)
hsa04924 Renin secretion - Homo sapiens (human)
hsa05010 Alzheimer's disease - Homo sapiens (human)
hsa05014 Amyotrophic lateral sclerosis (ALS) - Homo sapiens (human)
hsa05031 Amphetamine addiction - Homo sapiens (human)
hsa05152 Tuberculosis - Homo sapiens (human)
hsa05166 HTLV-I infection - Homo sapiens (human)