Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0006466
UniProt IDQ08209
Primary gene name(s)PPP3CA
Synonym gene name(s)CALNA, CNA
Protein nameSerine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
Protein functionCalcium-dependent, calmodulin-stimulated protein phosphatase. Many of the substrates contain a PxIxIT motif. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1. {ECO:0000269|PubMed:15671020, ECO:0000269|PubMed:17498738, ECO:0000269|PubMed:18838687}.
Subcellular locationCell membrane {ECO:0000250}. Cell membrane, sarcolemma {ECO:0000250}. Nucleus {ECO:0000250}. Note=Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q08209
Gene Ontology
(Biological Process)
Complete annatation
calcineurin-NFAT signaling cascade [GO:0033173];
calcium ion transport [GO:0006816];
cardiac muscle hypertrophy in response to stress [GO:0014898];
cellular response to drug [GO:0035690];
cellular response to glucose stimulus [GO:0071333];
dephosphorylation [GO:0016311];
excitatory postsynaptic potential [GO:0060079];
Fc-epsilon receptor signaling pathway [GO:0038095];
G1/S transition of mitotic cell cycle [GO:0000082];
modulation of synaptic transmission [GO:0050804];
multicellular organismal response to stress [GO:0033555];
negative regulation of chromatin binding [GO:0035562];
negative regulation of dendrite morphogenesis [GO:0050774];
negative regulation of insulin secretion [GO:0046676];
negative regulation of production of miRNAs involved in gene silencing by miRNA [GO:1903799];
positive regulation of cardiac muscle hypertrophy in response to stress [GO:1903244];
positive regulation of connective tissue replacement [GO:1905205];
positive regulation of NFAT protein import into nucleus [GO:0051533];
positive regulation of sequence-specific DNA binding transcription factor activity [GO:0051091];
positive regulation of transcription from RNA polymerase II promoter [GO:0045944];
protein dephosphorylation [GO:0006470];
protein import into nucleus [GO:0006606];
response to amphetamine [GO:0001975];
response to calcium ion [GO:0051592];
skeletal muscle fiber development [GO:0048741];
T cell activation [GO:0042110];
transition between fast and slow fiber [GO:0014883];
Wnt signaling pathway, calcium modulating pathway [GO:0007223]
Gene Ontology
(Molecular Function)
Complete annatation
calcium ion binding [GO:0005509];
calmodulin binding [GO:0005516];
calmodulin-dependent protein phosphatase activity [GO:0033192];
drug binding [GO:0008144];
enzyme binding [GO:0019899];
protein dimerization activity [GO:0046983];
protein serine/threonine phosphatase activity [GO:0004722]
Gene Ontology
(Cellular Component)
Complete annatation
calcineurin complex [GO:0005955];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
mitochondrion [GO:0005739];
nucleoplasm [GO:0005654];
postsynapse [GO:0098794];
sarcolemma [GO:0042383];
Z disc [GO:0030018]
Protein-protein interaction111522
Phylogenetic treeQ08209
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.266901737253820.0001675359797056460.000758744677320764
AZA vs. DISU0.1696184036938790.5039415138854650.936111234131953
AZA vs. IL70.01256123473643410.9480453723240370.999311006273513
AZA vs. SAHA0.299146975080970.2206948530668910.589221538112564
DISU vs. CD31.42457422472050.0001209450493256940.000670759997621599
DISU vs. IL7-0.165869343181290.5107505619101490.832649238171141
DISU vs. SAHA0.1300778966256270.6562872018642380.894401521498533
DMSO vs. AZA-0.1127552127210870.5025188333923711
DMSO vs. CD31.144345040578360.0004700413320583020.00176764170430812
DMSO vs. DISU-0.2837598034807250.2462503541882520.765784737436435
DMSO vs. IL70.1323198416228420.4630536255371510.876070959459407
DMSO vs. SAHA0.4040007796458340.08730669989124260.345349278677399
HIV vs. Mock in Activation0.1072929319665760.863550954241040.999983755607037
HIV vs. Mock in Latency-0.006771533444225580.967439988323940.999834320637052
IL7 vs. CD31.288100879713978.89810396635937e-050.000525584988240947
SAHA vs. CD31.540485038105212.54146977507475e-050.000143391798990552
SAHA vs. IL70.2822960231790740.247353045058540.489743058673525
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.49976 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.03 1.179 1.359 1.643 1.361
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB08231 MYRISTIC ACID experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1AUI X-ray 2.1Å A=1-521.
1M63 X-ray 2.8Å A/E=1-372.
1MF8 X-ray 3.1Å A=20-392.
2JOG NMR - A=21-347.
2JZI NMR - B=391-414.
2P6B X-ray 2.3Å A/C=1-380.
2R28 X-ray 1.8Å C/D=389-413.
2W73 X-ray 1.4Å K/L/M/O=395-411.
3LL8 X-ray 2.0Å A/C=14-370.
4F0Z X-ray 1.7Å A=1-370.
4Q5U X-ray 1.9Å C=391-414.
5C1V X-ray 3.3Å A/B=2-346.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat activates 12482669
Envelope surface glycoprotein gp120 regulated by 24086760

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04010 MAPK signaling pathway - Homo sapiens (human)
hsa04020 Calcium signaling pathway - Homo sapiens (human)
hsa04022 cGMP-PKG signaling pathway - Homo sapiens (human)
hsa04114 Oocyte meiosis - Homo sapiens (human)
hsa04310 Wnt signaling pathway - Homo sapiens (human)
hsa04360 Axon guidance - Homo sapiens (human)
hsa04370 VEGF signaling pathway - Homo sapiens (human)
hsa04380 Osteoclast differentiation - Homo sapiens (human)
hsa04650 Natural killer cell mediated cytotoxicity - Homo sapiens (human)
hsa04658 Th1 and Th2 cell differentiation - Homo sapiens (human)
hsa04659 Th17 cell differentiation - Homo sapiens (human)
hsa04660 T cell receptor signaling pathway - Homo sapiens (human)
hsa04662 B cell receptor signaling pathway - Homo sapiens (human)
hsa04720 Long-term potentiation - Homo sapiens (human)
hsa04724 Glutamatergic synapse - Homo sapiens (human)
hsa04728 Dopaminergic synapse - Homo sapiens (human)
hsa04921 Oxytocin signaling pathway - Homo sapiens (human)
hsa04922 Glucagon signaling pathway - Homo sapiens (human)
hsa04924 Renin secretion - Homo sapiens (human)
hsa05010 Alzheimer's disease - Homo sapiens (human)
hsa05014 Amyotrophic lateral sclerosis (ALS) - Homo sapiens (human)
hsa05031 Amphetamine addiction - Homo sapiens (human)
hsa05152 Tuberculosis - Homo sapiens (human)
hsa05166 HTLV-I infection - Homo sapiens (human)