Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0006434
UniProt IDO43447
Primary gene name(s)PPIH
Synonym gene name(s)CYP20, CYPH
Protein namePeptidyl-prolyl cis-trans isomerase H
Protein functionPPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Participates in pre-mRNA splicing. May play a role in the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome. May act as a chaperone. {ECO:0000269|PubMed:11823439, ECO:0000269|PubMed:12875835, ECO:0000269|PubMed:9570313}.
Subcellular locationNucleus speckle {ECO:0000269|PubMed:9570313}. Cytoplasm {ECO:0000269|PubMed:9570313}. Note=Colocalizes with spliceosomal snRNPs. A small proportion may also be cytoplasmic.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O43447
Gene Ontology
(Biological Process)
Complete annatation
mRNA splicing, via spliceosome [GO:0000398];
positive regulation of viral genome replication [GO:0045070];
protein complex assembly [GO:0006461];
protein folding [GO:0006457]
Gene Ontology
(Molecular Function)
Complete annatation
cyclosporin A binding [GO:0016018];
peptidyl-prolyl cis-trans isomerase activity [GO:0003755];
ribonucleoprotein complex binding [GO:0043021]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
nuclear speck [GO:0016607];
nucleoplasm [GO:0005654];
spliceosomal complex [GO:0005681];
U4/U6 snRNP [GO:0071001];
U4/U6 x U5 tri-snRNP complex [GO:0046540]
Protein-protein interaction115728
Phylogenetic treeO43447
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.502053455919480.1292164822986190.216010516992544
AZA vs. DISU0.4166909333558320.1025155600270440.645766632614985
AZA vs. IL70.2490051778071540.1994228382464340.964360158656313
AZA vs. SAHA-0.1228456642442220.6173322193352240.875662545804005
DISU vs. CD3-0.09745203127457450.788138031203660.8541514618135
DISU vs. IL7-0.1770171893841470.4847898183797310.817468380248929
DISU vs. SAHA-0.5374347992030370.06918204910457270.332051803202234
DMSO vs. AZA-0.05673714933105850.7384226318594521
DMSO vs. CD3-0.567758502625830.07917377603673290.141154193608684
DMSO vs. DISU-0.4747461817057040.05395659538418250.452742701803803
DMSO vs. IL70.3127254627932970.0851455059991770.546491570583801
DMSO vs. SAHA-0.0726200703810060.7597967984352150.930362166819721
HIV vs. Mock in Activation-0.1111418509903070.8583081735423590.999983755607037
HIV vs. Mock in Latency-0.03477085409314860.836004032504780.999834320637052
IL7 vs. CD3-0.2460849294035710.4466674991933510.580949108235515
SAHA vs. CD3-0.6481679209372180.07207500302104890.133979313621217
SAHA vs. IL7-0.3738067091950360.1280543123302480.332923543302201
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.588171 0.00154878
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.012 0.873 0.925 0.848 0.82
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00172 L-Proline approved, nutraceutical unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1MZW X-ray 2.0Å A=1-177.
1QOI X-ray 2.0Å A=1-177.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
retropepsin cleaves 22944692

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03040 Spliceosome - Homo sapiens (human)