Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0006413
UniProt IDQ9BUI4
Primary gene name(s)POLR3C
Synonym gene name(s)unknown
Protein nameDNA-directed RNA polymerase III subunit RPC3
Protein functionDNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs, EBERs induce type I interferon and NF- Kappa-B through the RIG-I pathway. {ECO:0000269|PubMed:19609254, ECO:0000269|PubMed:19631370}.
Subcellular locationNucleus {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9BUI4
Gene Ontology
(Biological Process)
Complete annatation
defense response to virus [GO:0051607];
innate immune response [GO:0045087];
positive regulation of innate immune response [GO:0045089];
positive regulation of interferon-beta production [GO:0032728];
positive regulation of type I interferon production [GO:0032481];
regulation of transcription from RNA polymerase III promoter [GO:0006359];
transcription from RNA polymerase III promoter [GO:0006383]
Gene Ontology
(Molecular Function)
Complete annatation
DNA binding [GO:0003677];
DNA-directed RNA polymerase activity [GO:0003899]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
DNA-directed RNA polymerase III complex [GO:0005666];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction115868
Phylogenetic treeQ9BUI4
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.087238846347670.00104621399527050.00378960282064814
AZA vs. DISU-0.04932571105488450.8464899327783950.987834633347355
AZA vs. IL70.06696602990096430.7311084107680130.999311006273513
AZA vs. SAHA-0.07824545485389730.750528916478720.931686806143475
DISU vs. CD3-1.14941019453930.001762626680149480.00673313339618912
DISU vs. IL70.1075081225270910.6715151175188250.909630636307924
DISU vs. SAHA-0.02762840742608980.9248614596809720.982806795139101
DMSO vs. AZA-0.01867355837725650.9129251756926651
DMSO vs. CD3-1.119268103010540.0005531741531413780.00204354306451076
DMSO vs. DISU0.02824668404455660.9084773584020110.988283279918411
DMSO vs. IL70.09323018829599760.6091854796704650.914597261829725
DMSO vs. SAHA-0.06582745255762920.7818724905555160.938799460769496
HIV vs. Mock in Activation-0.03730659351695890.9521852029600070.999983755607037
HIV vs. Mock in Latency0.04442194804203010.7916113500019820.999834320637052
IL7 vs. CD3-1.011986374150250.001842098926130410.00719139008380307
SAHA vs. CD3-1.19009562810740.0009005783746631750.00327423790257516
SAHA vs. IL7-0.1491554616085380.5434544774172180.759261476500797
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.0135794 0.994033
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.948 1.018 0.753 0.658 0.883
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2XUB X-ray 2.8Å A=1-534.
2XV4 X-ray 2.9Å S=1-534.
5AFQ X-ray 7.0Å A/B=1-534.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat activates 1403646

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00230 Purine metabolism - Homo sapiens (human)
hsa00240 Pyrimidine metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa03020 RNA polymerase - Homo sapiens (human)
hsa04623 Cytosolic DNA-sensing pathway - Homo sapiens (human)
hsa05169 Epstein-Barr virus infection - Homo sapiens (human)