Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0006398
UniProt IDP56282
Primary gene name(s)POLE2
Synonym gene name(s)DPE2
Protein nameDNA polymerase epsilon subunit 2
Protein functionParticipates in DNA repair and in chromosomal DNA replication.
Subcellular locationNucleus.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P56282
Gene Ontology
(Biological Process)
Complete annatation
DNA-dependent DNA replication [GO:0006261];
DNA repair [GO:0006281];
DNA replication [GO:0006260];
DNA replication initiation [GO:0006270];
error-prone translesion synthesis [GO:0042276];
G1/S transition of mitotic cell cycle [GO:0000082];
telomere maintenance via recombination [GO:0000722]
Gene Ontology
(Molecular Function)
Complete annatation
DNA binding [GO:0003677];
DNA-directed DNA polymerase activity [GO:0003887]
Gene Ontology
(Cellular Component)
Complete annatation
epsilon DNA polymerase complex [GO:0008622];
intracellular membrane-bounded organelle [GO:0043231];
nucleoplasm [GO:0005654]
Protein-protein interaction111423
Phylogenetic treeP56282
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD32.794884256276430.004421071657979340.0130963820811841
AZA vs. DISU0.1429916660605490.6074253325762070.955602906116453
AZA vs. IL71.157492701478980.001281024303133930.0965443966056884
AZA vs. SAHA-0.1896782655734750.4929529860556630.816959202718489
DISU vs. CD3-2.665692260905260.005887914297433410.0186146015351324
DISU vs. IL71.005135649295420.004232612978085860.0674985099133832
DISU vs. SAHA-0.3324554383289820.2909380623743540.67333039589126
DMSO vs. AZA-0.1076737493410860.6095642811377471
DMSO vs. CD3-2.912647178869870.003653711318242550.0105204818442898
DMSO vs. DISU-0.2513990225612550.3505960813067420.843687444668617
DMSO vs. IL71.269753169337160.0003925755450479020.0300409966578534
DMSO vs. SAHA-0.08834437574062310.7418733022912070.92450038820069
HIV vs. Mock in Activation-0.1594880692614840.9289434466201840.999983755607037
HIV vs. Mock in Latency0.185310816217720.4730621128409740.999834320637052
IL7 vs. CD3-1.63240507312790.08638732453950840.168908566971593
SAHA vs. CD3-3.01448179527520.001991706466132340.00644652415047558
SAHA vs. IL7-1.353031323703450.0004213826836046990.00601507819380419
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
2 0.000331038 1.2 0.16224508 1.3 0.320054468
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.748784 0.80398
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.993 1.045 1.02 0.951 1.246
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
205909_at 2.31 No upregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00242 Cladribine approved, investigational yes inhibitor

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2V6Z NMR - M=1-73.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00230 Purine metabolism - Homo sapiens (human)
hsa00240 Pyrimidine metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa03030 DNA replication - Homo sapiens (human)
hsa03410 Base excision repair - Homo sapiens (human)
hsa03420 Nucleotide excision repair - Homo sapiens (human)
hsa05166 HTLV-I infection - Homo sapiens (human)