Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0006397
UniProt IDQ07864
Primary gene name(s)POLE
Synonym gene name(s)POLE1
Protein nameDNA polymerase epsilon catalytic subunit A
Protein functionParticipates in DNA repair and in chromosomal DNA replication.
Subcellular locationNucleus.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q07864
Gene Ontology
(Biological Process)
Complete annatation
base-excision repair, gap-filling [GO:0006287];
DNA replication [GO:0006260];
DNA replication initiation [GO:0006270];
DNA replication proofreading [GO:0045004];
DNA synthesis involved in DNA repair [GO:0000731];
embryonic organ development [GO:0048568];
G1/S transition of mitotic cell cycle [GO:0000082];
leading strand elongation [GO:0006272];
nucleotide-excision repair, DNA gap filling [GO:0006297];
telomere maintenance via recombination [GO:0000722]
Gene Ontology
(Molecular Function)
Complete annatation
3'-5' exonuclease activity [GO:0008408];
4 iron, 4 sulfur cluster binding [GO:0051539];
chromatin binding [GO:0003682];
DNA binding [GO:0003677];
DNA-directed DNA polymerase activity [GO:0003887];
nucleotide binding [GO:0000166];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
epsilon DNA polymerase complex [GO:0008622];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
plasma membrane [GO:0005886]
Protein-protein interaction111422
Phylogenetic treeQ07864
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.445426687016770.2136535497747780.320927476799428
AZA vs. DISU0.4969926167473380.04995429191993790.501327357161208
AZA vs. IL70.2193881158600130.2552674448907750.999311006273513
AZA vs. SAHA-0.1017175214145770.6775130281182250.906035431676199
DISU vs. CD30.03816494066740280.9179617975322430.946903448702294
DISU vs. IL7-0.2868025877949660.2551069647891380.64092186091893
DISU vs. SAHA-0.5959899524906320.04236496824708810.254375472689986
DMSO vs. AZA0.1607358617243390.3380612616275241
DMSO vs. CD3-0.2978260822756010.384703830411380.499230183921736
DMSO vs. DISU-0.3384462855448790.1655650119310330.674146286131372
DMSO vs. IL70.06612879159868310.7134444292482340.941906863374828
DMSO vs. SAHA-0.2675173192889520.2584650306187520.607126944584665
HIV vs. Mock in Activation0.1349714335145470.8613487404518430.999983755607037
HIV vs. Mock in Latency0.06696973580496140.6855781025212010.999834320637052
IL7 vs. CD3-0.2198240185850620.5143365363159350.642515714560757
SAHA vs. CD3-0.5707612627940070.1655618444662690.260493896658619
SAHA vs. IL7-0.3236310759844180.1884476773431190.418578575554726
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
2 0.000331038 1.2 0.16224508 1.3 0.320054468
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.509433 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.953 1.106 1.121 0.956 1.263
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00242 Cladribine approved, investigational yes inhibitor

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
retropepsin interacts with 22190034
HIV-1 virus replication enhanced by expression of human gene 18976975
Tat interacts with 9121429

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00230 Purine metabolism - Homo sapiens (human)
hsa00240 Pyrimidine metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa03030 DNA replication - Homo sapiens (human)
hsa03410 Base excision repair - Homo sapiens (human)
hsa03420 Nucleotide excision repair - Homo sapiens (human)
hsa05166 HTLV-I infection - Homo sapiens (human)