Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0006361
UniProt IDP19174
Primary gene name(s)PLCG1
Synonym gene name(s)PLC1
Protein name1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1
Protein functionMediates the production of the second messenger molecules diacylglycerol, DAG and inositol 1,4,5-trisphosphate, IP3. Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, FGFR1, FGFR2, FGFR3 and FGFR4. Plays a role in actin reorganization and cell migration. {ECO:0000269|PubMed:17229814}.
Subcellular locationCell projection, lamellipodium. Cell projection, ruffle. Note=Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor, EGF treatment.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P19174
Gene Ontology
(Biological Process)
Complete annatation
activation of MAPKK activity [GO:0000186];
calcium-mediated signaling [GO:0019722];
cell migration [GO:0016477];
cellular response to epidermal growth factor stimulus [GO:0071364];
epidermal growth factor receptor signaling pathway [GO:0007173];
Fc-epsilon receptor signaling pathway [GO:0038095];
Fc-gamma receptor signaling pathway involved in phagocytosis [GO:0038096];
inositol phosphate metabolic process [GO:0043647];
in utero embryonic development [GO:0001701];
leukocyte migration [GO:0050900];
phospholipid catabolic process [GO:0009395];
positive regulation of angiogenesis [GO:0045766];
positive regulation of blood vessel endothelial cell migration [GO:0043536];
positive regulation of epithelial cell migration [GO:0010634];
positive regulation of release of sequestered calcium ion into cytosol [GO:0051281];
signal transduction [GO:0007165];
T cell receptor signaling pathway [GO:0050852];
viral process [GO:0016032]
Gene Ontology
(Molecular Function)
Complete annatation
calcium ion binding [GO:0005509];
neurotrophin TRKA receptor binding [GO:0005168];
phosphatidylinositol phospholipase C activity [GO:0004435];
phospholipase C activity [GO:0004629];
protein kinase binding [GO:0019901];
receptor signaling protein activity [GO:0005057]
Gene Ontology
(Cellular Component)
Complete annatation
cell-cell junction [GO:0005911];
cell projection [GO:0042995];
COP9 signalosome [GO:0008180];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
lamellipodium [GO:0030027];
plasma membrane [GO:0005886];
ruffle [GO:0001726]
Protein-protein interaction111351
Phylogenetic treeP19174
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-2.08741246178688.66430482915348e-101.26908068401362e-08
AZA vs. DISU0.03292419194999190.8966029907674130.993937521279788
AZA vs. IL70.09936864812643020.6039047157551030.999311006273513
AZA vs. SAHA-0.1785644259258970.4630163862593410.801741196382099
DISU vs. CD32.107637023147192.26882426179742e-083.24788764694533e-07
DISU vs. IL70.05739627941117050.8197994974537620.961243368243154
DISU vs. SAHA-0.2092110996388060.474044615030340.806461404494033
DMSO vs. AZA0.1267021948036390.4472682908230831
DMSO vs. CD32.200525705815664.25344204302291e-117.14360137994874e-10
DMSO vs. DISU0.09152352454189890.7078359993151090.960763093481455
DMSO vs. IL7-0.01975736265323450.9121459702858050.981835094756053
DMSO vs. SAHA-0.3103372814395570.1872120913077960.521849711198099
HIV vs. Mock in Activation0.04063416632246070.9479014690070630.999983755607037
HIV vs. Mock in Latency-0.05228431202264130.7506808526049550.999834320637052
IL7 vs. CD32.193403335263335.8427929161553e-111.2932219915596e-09
SAHA vs. CD31.885322012517922.38645537109505e-072.27188139244488e-06
SAHA vs. IL7-0.280742569546470.2481476968075770.490721327652865
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.528252 0.0108507
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.004 0.992 1.033 1.036 1.071
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1HSQ NMR - A=790-851.
2HSP NMR - A=790-851.
4EY0 X-ray 2.8Å A/B/C/D=545-790.
4FBN X-ray 2.4Å A=545-790.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat activates 7589147
Nef inhibits 21176845
Tat cooperates with 20336759
Envelope surface glycoprotein gp160; precursor inhibits 9344703
Envelope surface glycoprotein gp120 activates 19710135
Pr55(Gag) interacts with 21762810
Envelope surface glycoprotein gp120 inactivates 1979339

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00562 Inositol phosphate metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa01521 EGFR tyrosine kinase inhibitor resistance - Homo sapiens (human)
hsa04012 ErbB signaling pathway - Homo sapiens (human)
hsa04014 Ras signaling pathway - Homo sapiens (human)
hsa04015 Rap1 signaling pathway - Homo sapiens (human)
hsa04020 Calcium signaling pathway - Homo sapiens (human)
hsa04064 NF-kappa B signaling pathway - Homo sapiens (human)
hsa04066 HIF-1 signaling pathway - Homo sapiens (human)
hsa04070 Phosphatidylinositol signaling system - Homo sapiens (human)
hsa04072 Phospholipase D signaling pathway - Homo sapiens (human)
hsa04360 Axon guidance - Homo sapiens (human)
hsa04370 VEGF signaling pathway - Homo sapiens (human)
hsa04650 Natural killer cell mediated cytotoxicity - Homo sapiens (human)
hsa04658 Th1 and Th2 cell differentiation - Homo sapiens (human)
hsa04659 Th17 cell differentiation - Homo sapiens (human)
hsa04660 T cell receptor signaling pathway - Homo sapiens (human)
hsa04664 Fc epsilon RI signaling pathway - Homo sapiens (human)
hsa04666 Fc gamma R-mediated phagocytosis - Homo sapiens (human)
hsa04670 Leukocyte transendothelial migration - Homo sapiens (human)
hsa04722 Neurotrophin signaling pathway - Homo sapiens (human)
hsa04750 Inflammatory mediator regulation of TRP channels - Homo sapiens (human)
hsa04919 Thyroid hormone signaling pathway - Homo sapiens (human)
hsa04933 AGE-RAGE signaling pathway in diabetic complications - Homo sapiens (human)
hsa05110 Vibrio cholerae infection - Homo sapiens (human)
hsa05120 Epithelial cell signaling in Helicobacter pylori infection - Homo sapiens (human)
hsa05169 Epstein-Barr virus infection - Homo sapiens (human)
hsa05200 Pathways in cancer - Homo sapiens (human)
hsa05205 Proteoglycans in cancer - Homo sapiens (human)
hsa05206 MicroRNAs in cancer - Homo sapiens (human)
hsa05214 Glioma - Homo sapiens (human)
hsa05223 Non-small cell lung cancer - Homo sapiens (human)
hsa05231 Choline metabolism in cancer - Homo sapiens (human)