Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0006355
UniProt IDQ99640
Primary gene name(s)PKMYT1
Synonym gene name(s)MYT1
Protein nameMembrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
Protein functionActs as a negative regulator of entry into mitosis, G2 to M transition by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development. {ECO:0000269|PubMed:10373560, ECO:0000269|PubMed:9001210}.
Subcellular locationEndoplasmic reticulum membrane {ECO:0000269|PubMed:9001210};
Peripheral membrane protein {ECO:0000269|PubMed:9001210}. Golgi apparatus membrane {ECO:0000269|PubMed:9001210};
Peripheral membrane protein {ECO:0000269|PubMed:9001210}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q99640
Gene Ontology
(Biological Process)
Complete annatation
G1/S transition of mitotic cell cycle [GO:0000082];
G2/M transition of mitotic cell cycle [GO:0000086];
mitotic nuclear division [GO:0007067];
negative regulation of phosphatase activity [GO:0010923];
regulation of cell cycle [GO:0051726];
regulation of cyclin-dependent protein serine/threonine kinase activity [GO:0000079];
regulation of mitotic nuclear division [GO:0007088]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
kinase activity [GO:0016301];
metal ion binding [GO:0046872];
protein kinase activity [GO:0004672];
protein serine/threonine kinase activity [GO:0004674]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
endoplasmic reticulum [GO:0005783];
endoplasmic reticulum membrane [GO:0005789];
Golgi apparatus [GO:0005794];
Golgi membrane [GO:0000139];
membrane [GO:0016020];
nucleoplasm [GO:0005654]
Protein-protein interaction114544
Phylogenetic treeQ99640
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.614730513837110.1679650647337120.266480995333317
AZA vs. DISU-0.4089077390455920.1289503399577050.694957963719144
AZA vs. IL70.4982612397537330.220982963917550.989265982093417
AZA vs. SAHA0.4801339521784760.103851041361810.408947387013563
DISU vs. CD3-2.038701355643370.07265790572473740.145142495480346
DISU vs. IL70.8997902237687050.04218504268226320.271529969733256
DISU vs. SAHA0.8927344025032860.01155301190649250.113341304655618
DMSO vs. AZA0.01514547904075920.9361503291207921
DMSO vs. CD3-1.615246869006170.1565871621503270.245242291069204
DMSO vs. DISU0.4205577659163840.1041451835009110.576642333682909
DMSO vs. IL70.4934606820267910.2157386865897540.724804783238115
DMSO vs. SAHA0.4627870753952180.1063683781711180.386851640117024
HIV vs. Mock in Activation0.1961265259646470.9226457805790610.999983755607037
HIV vs. Mock in Latency0.1084686794833060.7333149364324560.999834320637052
IL7 vs. CD3-1.113296382799620.3136067501101820.451479899179634
SAHA vs. CD3-1.158321990469080.3787630529931990.495267610291323
SAHA vs. IL7-0.01903415049134940.9689104431108520.98858508321734
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.766392 0.00322755
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
0.018 0.01

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.841 1.159 1.351 1.154 1.14
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3P1A X-ray 1.7Å A=75-362.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04110 Cell cycle - Homo sapiens (human)
hsa04114 Oocyte meiosis - Homo sapiens (human)
hsa04914 Progesterone-mediated oocyte maturation - Homo sapiens (human)