Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0006326
UniProt IDQ8N2W9
Primary gene name(s)PIAS4
Synonym gene name(s)PIASG
Protein nameE3 SUMO-protein ligase PIAS4
Protein functionFunctions as an E3-type small ubiquitin-like modifier, SUMO ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Mediates sumoylation of CEBPA, PARK7, HERC2, MYB, TCF4 and RNF168. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. {ECO:0000269|PubMed:12511558, ECO:0000269|PubMed:12631292, ECO:0000269|PubMed:12727872, ECO:0000269|PubMed:15831457, ECO:0000269|PubMed:15976810, ECO:0000269|PubMed:21965678, ECO:0000269|PubMed:22508508}.
Subcellular locationNucleus, PML body {ECO:0000269|PubMed:11248056, ECO:0000269|PubMed:12727872, ECO:0000269|PubMed:15831457}. Note=Colocalizes with SUMO1 and TCF7L2/TCF4 and LEF1 in a subset of PML, promyelocytic leukemia nuclear bodies.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q8N2W9
Gene Ontology
(Biological Process)
Complete annatation
double-strand break repair via nonhomologous end joining [GO:0006303];
negative regulation of NF-kappaB transcription factor activity [GO:0032088];
negative regulation of transcription, DNA-templated [GO:0045892];
negative regulation of transcription from RNA polymerase II promoter [GO:0000122];
negative regulation of tumor necrosis factor-mediated signaling pathway [GO:0010804];
positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage [GO:1902231];
positive regulation of keratinocyte apoptotic process [GO:1902174];
positive regulation of protein sumoylation [GO:0033235];
protein sumoylation [GO:0016925];
transcription, DNA-templated [GO:0006351];
Wnt signaling pathway [GO:0016055]
Gene Ontology
(Molecular Function)
Complete annatation
DNA binding [GO:0003677];
ligase activity [GO:0016874];
SUMO ligase activity [GO:0061665];
SUMO transferase activity [GO:0019789];
transcription corepressor activity [GO:0003714];
ubiquitin protein ligase binding [GO:0031625];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
nuclear matrix [GO:0016363];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
PML body [GO:0016605];
transferase complex [GO:1990234]
Protein-protein interaction119624
Phylogenetic treeQ8N2W9
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.187208875792540.5705852552310110.679347576178356
AZA vs. DISU0.3165214208794050.2232663696644750.811184149496501
AZA vs. IL7-0.1007481180673010.6074267874106290.999311006273513
AZA vs. SAHA-0.1896085401228240.442984812765020.788183866617569
DISU vs. CD30.11703372151070.7522538511465350.827578058107055
DISU vs. IL7-0.4273231686939050.1100517660318040.433963575691565
DISU vs. SAHA-0.5033465463439140.08964775420552010.381975358551638
DMSO vs. AZA-0.01487298889013840.9310787063213491
DMSO vs. CD3-0.2134142144796750.5085944586566420.616087576080541
DMSO vs. DISU-0.3333684102455880.1778137476009520.690311335740057
DMSO vs. IL7-0.07832232203545810.6695061917663990.93213494903488
DMSO vs. SAHA-0.1796604676197570.4519531855169230.782003654288608
HIV vs. Mock in Activation0.454945939986740.4685981480566610.999983755607037
HIV vs. Mock in Latency0.004476984323706610.9789211239334880.999834320637052
IL7 vs. CD3-0.2839744396529530.3820171607043250.518705041284235
SAHA vs. CD3-0.3996688685204670.2613379501694960.372562946651623
SAHA vs. IL7-0.09074386035774080.7130424513881030.868774833597586
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.0999768 0.625265
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.036 0.945 0.818 0.724 0.926
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04064 NF-kappa B signaling pathway - Homo sapiens (human)
hsa04120 Ubiquitin mediated proteolysis - Homo sapiens (human)
hsa04630 Jak-STAT signaling pathway - Homo sapiens (human)