Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0006325
UniProt IDO75928
Primary gene name(s)PIAS2
Synonym gene name(s)PIASX
Protein nameE3 SUMO-protein ligase PIAS2
Protein functionFunctions as an E3-type small ubiquitin-like modifier, SUMO ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha. Isoform PIAS2-alpha sumoylates PML at'Lys-65' and 'Lys-160'. {ECO:0000269|PubMed:15920481, ECO:0000269|PubMed:15976810, ECO:0000269|PubMed:22406621}.
Subcellular locationNucleus speckle {ECO:0000250|UniProtKB:Q8C5D8}. Nucleus, PML body {ECO:0000269|PubMed:12716907, ECO:0000269|PubMed:22406621}. Nucleus {ECO:0000269|PubMed:11477070, ECO:0000269|PubMed:12393906}. Note=Colocalizes at least partially with promyelocytic leukemia nuclear bodies, PML NBs, PubMed:22406621. Colocalizes with SUMO1 in nuclear granules, By similarity. {ECO:0000250|UniProtKB:Q8C5D8, ECO:0000269|PubMed:22406621}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O75928
Gene Ontology
(Biological Process)
Complete annatation
androgen receptor signaling pathway [GO:0030521];
negative regulation of androgen receptor signaling pathway [GO:0060766];
negative regulation of sequence-specific DNA binding transcription factor activity [GO:0043433];
positive regulation of transcription, DNA-templated [GO:0045893];
positive regulation of transcription from RNA polymerase II promoter [GO:0045944];
protein sumoylation [GO:0016925];
regulation of osteoblast differentiation [GO:0045667];
transcription, DNA-templated [GO:0006351]
Gene Ontology
(Molecular Function)
Complete annatation
androgen receptor binding [GO:0050681];
DNA binding [GO:0003677];
ligase activity [GO:0016874];
SUMO ligase activity [GO:0061665];
SUMO transferase activity [GO:0019789];
transcription coactivator activity [GO:0003713];
transcription factor binding [GO:0008134];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
nuclear speck [GO:0016607];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
PML body [GO:0016605]
Protein-protein interaction114523
Phylogenetic treeO75928
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.02694714518888760.9352907983652710.958437783040615
AZA vs. DISU-0.06824581470584190.8340698286245170.986138307819745
AZA vs. IL70.1328687257659860.6799901135824730.999311006273513
AZA vs. SAHA0.1905322753164890.5380705210933210.84256090305375
DISU vs. CD3-0.05548547172941880.8795498651878920.919504450228329
DISU vs. IL70.1934616763817930.5330813935278810.845206826471239
DISU vs. SAHA0.2582336083418930.3877482793793750.753702163765953
DMSO vs. AZA-0.06812777894188520.8162290594843171
DMSO vs. CD3-0.05873212963241120.8562536548280170.901385855808225
DMSO vs. DISU-0.003439167008349970.9900447049916210.99806609310612
DMSO vs. IL70.2090188982951290.4442112854372260.866592093088172
DMSO vs. SAHA0.2509980999590280.3312819885535480.685429886016738
HIV vs. Mock in Activation-0.3014865533417290.6328661296740150.999983755607037
HIV vs. Mock in Latency-0.4799322088024880.006037695098432440.232767815636242
IL7 vs. CD30.1710342439687230.5993480693644510.71612948470316
SAHA vs. CD30.1900878823330030.5952776400682040.693639065632709
SAHA vs. IL70.05083429738153650.8611696023826860.94206569383288
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0255181 0.909799
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.022 1.062 1.086 0.966 0.962
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2ASQ NMR - B=466-488.
4FO9 X-ray 2.3Å A=147-488.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04120 Ubiquitin mediated proteolysis - Homo sapiens (human)
hsa04630 Jak-STAT signaling pathway - Homo sapiens (human)
hsa05200 Pathways in cancer - Homo sapiens (human)
hsa05222 Small cell lung cancer - Homo sapiens (human)