Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0006324
UniProt IDO75925
Primary gene name(s)PIAS1
Synonym gene name(s)DDXBP1
Protein nameE3 SUMO-protein ligase PIAS1
Protein functionFunctions as an E3-type small ubiquitin-like modifier, SUMO ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma, IFN-gamma signaling. Sumoylates PML, at'Lys-65' and 'Lys-160' and PML-RAR and promotes their ubiquitin-mediated degradation. PIAS1-mediated sumoylation of PML promotes its interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation, By similarity. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Plays a dynamic role in adipogenesis by promoting the SUMOylation and degradation of CEBPB, By similarity. {ECO:0000250|UniProtKB:O88907, ECO:0000269|PubMed:14500712, ECO:0000269|PubMed:19136629, ECO:0000269|PubMed:21965678}.
Subcellular locationNucleus speckle {ECO:0000269|PubMed:12393906, ECO:0000269|PubMed:19136629, ECO:0000269|PubMed:9177271}. Nucleus, PML body {ECO:0000250}. Note=Interaction with CSRP2 may induce a partial redistribution along the cytoskeleton.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O75925
Gene Ontology
(Biological Process)
Complete annatation
androgen receptor signaling pathway [GO:0030521];
fat cell differentiation [GO:0045444];
G1/S transition of mitotic cell cycle [GO:0000082];
JAK-STAT cascade [GO:0007259];
negative regulation of apoptotic process [GO:0043066];
negative regulation of transcription from RNA polymerase II promoter [GO:0000122];
positive regulation of proteasomal ubiquitin-dependent protein catabolic process [GO:0032436];
positive regulation of protein sumoylation [GO:0033235];
positive regulation of smooth muscle cell differentiation [GO:0051152];
positive regulation of transcription, DNA-templated [GO:0045893];
protein-DNA complex assembly [GO:0065004];
protein sumoylation [GO:0016925];
regulation of cell proliferation [GO:0042127];
regulation of interferon-gamma-mediated signaling pathway [GO:0060334];
spermatogenesis [GO:0007283];
transcription, DNA-templated [GO:0006351];
visual learning [GO:0008542]
Gene Ontology
(Molecular Function)
Complete annatation
androgen receptor binding [GO:0050681];
DNA binding [GO:0003677];
enzyme binding [GO:0019899];
ligase activity [GO:0016874];
SUMO ligase activity [GO:0061665];
SUMO transferase activity [GO:0019789];
transcription coactivator activity [GO:0003713];
transcription corepressor activity [GO:0003714];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
nuclear speck [GO:0016607];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
PML body [GO:0016605]
Protein-protein interaction114124
Phylogenetic treeO75925
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.3184412917171230.3328551200376810.453679903165846
AZA vs. DISU0.212800241718970.4026209444011630.905373601350453
AZA vs. IL7-0.0827031112317190.6691068276734210.999311006273513
AZA vs. SAHA0.0133174145657350.9566458363282460.990779256780515
DISU vs. CD30.5182365889542990.1577918072418280.26596821003727
DISU vs. IL7-0.3046076913143950.2289041828352430.61270073796898
DISU vs. SAHA-0.1979186425402510.5002150240071750.825168632531976
DMSO vs. AZA-0.008500947654210280.9598806038341981
DMSO vs. CD30.2984837564978490.3525308453386450.46686150812719
DMSO vs. DISU-0.2231465829491160.3628935403179490.848481264667731
DMSO vs. IL7-0.06699937418868970.711313492858220.941316067698277
DMSO vs. SAHA0.01532793696055450.9483645737117480.987880038071194
HIV vs. Mock in Activation0.1729528191512520.7820262485922390.999983755607037
HIV vs. Mock in Latency0.09784377388932120.5555750536591410.999834320637052
IL7 vs. CD30.2428084429737650.4519234809023460.585983322395426
SAHA vs. CD30.307378966055680.3851879233801220.501753133835114
SAHA vs. IL70.09270925788009530.7047177629425950.864178536857342
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.0674062 0.720649
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.235 1.521 0.758 0.823 3.159
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1V66 NMR - A=1-65.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04120 Ubiquitin mediated proteolysis - Homo sapiens (human)
hsa04630 Jak-STAT signaling pathway - Homo sapiens (human)
hsa05160 Hepatitis C - Homo sapiens (human)