Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0006253
UniProt IDP30101
Primary gene name(s)PDIA3
Synonym gene name(s)ERP57, ERP60, GRP58
Protein nameProtein disulfide-isomerase A3
Protein functionunknown
Subcellular locationEndoplasmic reticulum {ECO:0000269|PubMed:23826168}. Endoplasmic reticulum lumen {ECO:0000250}. Melanosome {ECO:0000269|PubMed:12643545, ECO:0000269|PubMed:17081065}. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV, PubMed:12643545. {ECO:0000269|PubMed:12643545}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P30101
Gene Ontology
(Biological Process)
Complete annatation
antigen processing and presentation of peptide antigen via MHC class I [GO:0002474];
cell redox homeostasis [GO:0045454];
positive regulation of extrinsic apoptotic signaling pathway [GO:2001238];
protein folding [GO:0006457];
protein folding in endoplasmic reticulum [GO:0034975];
protein import into nucleus [GO:0006606];
protein retention in ER lumen [GO:0006621];
response to endoplasmic reticulum stress [GO:0034976];
signal transduction [GO:0007165]
Gene Ontology
(Molecular Function)
Complete annatation
cysteine-type endopeptidase activity [GO:0004197];
disulfide oxidoreductase activity [GO:0015036];
phospholipase C activity [GO:0004629];
poly(A RNA binding [GO:0044822];
protein disulfide isomerase activity [GO:0003756]
Gene Ontology
(Cellular Component)
Complete annatation
cell surface [GO:0009986];
endoplasmic reticulum [GO:0005783];
endoplasmic reticulum lumen [GO:0005788];
extracellular exosome [GO:0070062];
focal adhesion [GO:0005925];
melanosome [GO:0042470];
myelin sheath [GO:0043209];
nucleus [GO:0005634]
Protein-protein interaction109180
Phylogenetic treeP30101
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      Yes - Two siRNA pools inhibit HIV replication and inhibition of Tat-mediated transactivation of the HIV LTR is also observed
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.3786482958987090.2474647143565020.359601636596722
AZA vs. DISU-0.03822648749795860.8796963575864210.991771899371402
AZA vs. IL70.1128088603737310.5559543393471790.999311006273513
AZA vs. SAHA-0.3545019832328690.1456047352638640.485585349417584
DISU vs. CD3-0.428019979025530.237490584989990.362488161010441
DISU vs. IL70.141635620903490.5734040314577970.863368710742884
DISU vs. SAHA-0.3150900167988470.2796481173366780.660215868673824
DMSO vs. AZA-0.07700800555725330.6442813990084651
DMSO vs. CD3-0.4648788622979770.1461453039893840.232220124944634
DMSO vs. DISU-0.04005344695144040.8693719488199420.985585524324111
DMSO vs. IL70.1967891939173550.2721354046000380.76921890271593
DMSO vs. SAHA-0.284536098542190.2266580132175220.572662986065054
HIV vs. Mock in Activation-0.05235405316775550.9328402286162520.999983755607037
HIV vs. Mock in Latency-0.05305595809388750.7467712515515220.999834320637052
IL7 vs. CD3-0.2584143132078810.4206084090857640.55744575311261
SAHA vs. CD3-0.757294938253710.03286189781481960.0700762416965235
SAHA vs. IL7-0.4701735712889810.05342635427409570.192578772442855
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.367497 0.00513273
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.015 0.974 1.14 1.215 0.938
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2ALB NMR - A=25-137.
2DMM NMR - A=357-485.
2H8L X-ray 2.0Å A/B/C=134-376.
3F8U X-ray 2.6Å A/C=25-505.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp120 cleaved by 12218051
12218052
15644496
16182193
22230366
retropepsin cleaves 22944692
Envelope surface glycoprotein gp160; precursor interacts with 22190034
Envelope surface glycoprotein gp120 interacts with 20458450
23206338
Vpr downregulates 23874603
Envelope transmembrane glycoprotein gp41 interacts with 22190034
Envelope surface glycoprotein gp120 co-localizes with 24825317
Envelope surface glycoprotein gp160; precursor processed by 17301129
HIV-1 virus replication enhanced by expression of human gene 18976975

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04141 Protein processing in endoplasmic reticulum - Homo sapiens (human)
hsa04612 Antigen processing and presentation - Homo sapiens (human)
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