Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0006206
UniProt IDQ8TEW0
Primary gene name(s)PARD3
Synonym gene name(s)PAR3, PAR3A
Protein namePartitioning defective 3 homolog
Protein functionAdapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions. Involved in Schwann cell peripheral myelination, By similarity. Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons. {ECO:0000250, ECO:0000269|PubMed:19812038}.
Subcellular locationCytoplasm {ECO:0000250}. Endomembrane system {ECO:0000269|PubMed:20332120}. Cell junction {ECO:0000269|PubMed:20332120}. Cell junction, tight junction {ECO:0000269|PubMed:20332120}. Cell membrane {ECO:0000269|PubMed:20332120}. Cytoplasm, cell cortex {ECO:0000250}. Cytoplasm, cytoskeleton {ECO:0000269|PubMed:20332120}. Note=Localized along the cell-cell contact region. Colocalizes with PARD6A and PRKCI at epithelial tight junctions. Colocalizes with the cortical actin that overlays the meiotic spindle during metaphase I and metaphase II. Colocalized with SIRT2 in internode region of myelin sheat, By similarity. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q8TEW0
Gene Ontology
(Biological Process)
Complete annatation
asymmetric cell division [GO:0008356];
axonogenesis [GO:0007409];
bicellular tight junction assembly [GO:0070830];
cell cycle [GO:0007049];
establishment of epithelial cell polarity [GO:0090162];
establishment or maintenance of cell polarity [GO:0007163];
myelination in peripheral nervous system [GO:0022011];
negative regulation of peptidyl-threonine phosphorylation [GO:0010801];
positive regulation of myelination [GO:0031643];
protein complex assembly [GO:0006461];
protein kinase C-activating G-protein coupled receptor signaling pathway [GO:0007205];
protein targeting to membrane [GO:0006612];
regulation of cellular localization [GO:0060341];
transforming growth factor beta receptor signaling pathway [GO:0007179]
Gene Ontology
(Molecular Function)
Complete annatation
phosphatidylinositol-3,4,5-trisphosphate binding [GO:0005547];
phosphatidylinositol-3-phosphate binding [GO:0032266];
phosphatidylinositol-4,5-bisphosphate binding [GO:0005546]
Gene Ontology
(Cellular Component)
Complete annatation
axonal growth cone [GO:0044295];
bicellular tight junction [GO:0005923];
cell-cell junction [GO:0005911];
cell cortex [GO:0005938];
cell junction [GO:0030054];
cytoskeleton [GO:0005856];
cytosol [GO:0005829];
endomembrane system [GO:0012505];
internode region of axon [GO:0033269];
neuronal cell body [GO:0043025];
plasma membrane [GO:0005886];
protein complex [GO:0043234]
Protein-protein interaction121134
Phylogenetic treeQ8TEW0
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.3939408271634590.5862755072409290.692829264947098
AZA vs. DISU-0.0112434150995890.9886427160157410.998932793566471
AZA vs. IL7unknownunknownunknown
AZA vs. SAHAunknownunknownunknown
DISU vs. CD3-0.4264527134062640.5827092013904130.696957748294778
DISU vs. IL7unknownunknownunknown
DISU vs. SAHAunknownunknownunknown
DMSO vs. AZA0.2046769307702020.7883270917482461
DMSO vs. CD3-0.2184355563250610.7581690021340850.825932172763757
DMSO vs. DISU0.2118382728473730.7873757741600190.973311777316403
DMSO vs. IL7-0.4663296176497020.5298993037644660.895628207088737
DMSO vs. SAHA-0.2186931062728770.7422552778638470.92450038820069
HIV vs. Mock in Activation-0.3748924057668390.7001353583430860.999983755607037
HIV vs. Mock in Latency0.04654060056177140.9279963814424160.999834320637052
IL7 vs. CD3unknownunknownunknown
SAHA vs. CD3unknownunknownunknown
SAHA vs. IL7unknownunknownunknown
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.212294 0.638802
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.929 0.93 0.821 0.904 1.025
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2KOM NMR - A=451-549.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04015 Rap1 signaling pathway - Homo sapiens (human)
hsa04062 Chemokine signaling pathway - Homo sapiens (human)
hsa04080 Neuroactive ligand-receptor interaction - Homo sapiens (human)
hsa04144 Endocytosis - Homo sapiens (human)
hsa04360 Axon guidance - Homo sapiens (human)
hsa04390 Hippo signaling pathway - Homo sapiens (human)
hsa04520 Adherens junction - Homo sapiens (human)
hsa04530 Tight junction - Homo sapiens (human)