Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0006155
UniProt IDQ13416
Primary gene name(s)ORC2
Synonym gene name(s)ORC2L
Protein nameOrigin recognition complex subunit 2
Protein functionComponent of the origin recognition complex, ORC that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K20me3 and H4K27me3. Stabilizes LRWD1, by protecting it from ubiquitin-mediated proteasomal degradation. Also stabilizes ORC3. {ECO:0000269|PubMed:22427655, ECO:0000269|PubMed:22935713}.
Subcellular locationNucleus.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q13416
Gene Ontology
(Biological Process)
Complete annatation
DNA replication [GO:0006260];
DNA replication initiation [GO:0006270];
G1/S transition of mitotic cell cycle [GO:0000082];
negative regulation of transcription from RNA polymerase II promoter [GO:0000122]
Gene Ontology
(Molecular Function)
Complete annatation
DNA replication origin binding [GO:0003688]
Gene Ontology
(Cellular Component)
Complete annatation
centrosome [GO:0005813];
condensed chromosome inner kinetochore [GO:0000939];
heterochromatin [GO:0000792];
membrane [GO:0016020];
nuclear chromosome, telomeric region [GO:0000784];
nuclear origin of replication recognition complex [GO:0005664];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
origin recognition complex [GO:0000808]
Protein-protein interaction111041
Phylogenetic treeQ13416
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.1796994573373010.5852469429706870.692047852224556
AZA vs. DISU0.07643006519789610.7637519568255170.976021571236163
AZA vs. IL70.109209629039480.5718389771989940.999311006273513
AZA vs. SAHA-0.2070681705438590.3977016244784960.758003154165496
DISU vs. CD3-0.1155295845413120.7498789264102180.825809098239891
DISU vs. IL70.02395346003102860.9245368304802730.985969662041662
DISU vs. SAHA-0.2829790168005460.3334924496029920.712714791260167
DMSO vs. AZA-0.03634627681550750.8294501489019671
DMSO vs. CD3-0.2270794978007940.4801573947186480.590062089194586
DMSO vs. DISU-0.1144041877592210.6409821059649420.9472626202031
DMSO vs. IL70.1526048011018480.3984978494924610.844797508240592
DMSO vs. SAHA-0.1781925223198470.4512915138069620.782003654288608
HIV vs. Mock in Activation-0.02631314205105640.9662733128974760.999983755607037
HIV vs. Mock in Latency-0.01856413313523790.911007858038750.999834320637052
IL7 vs. CD3-0.06193509867595750.847644307266020.901470587978601
SAHA vs. CD3-0.4121569505091270.2459243724626950.354920541372613
SAHA vs. IL7-0.3203034820918170.1899782520406530.420271818500266
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.402736 0.0407833
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.005 1.034 0.987 1.022 1.205
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
5C8H X-ray 2.0Å A=458-577.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04110 Cell cycle - Homo sapiens (human)