Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0006088
UniProt IDQ9UNZ2
Primary gene name(s)NSFL1C
Synonym gene name(s)UBXN2C
Protein nameNSFL1 cofactor p47
Protein functionReduces the ATPase activity of VCP. Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum, tER, By similarity. Inhibits the activity of CTSL, in vitro. {ECO:0000250}.
Subcellular locationNucleus {ECO:0000250}. Golgi apparatus, Golgi stack {ECO:0000250}. Chromosome {ECO:0000250}. Note=Predominantly nuclear in interphase cells. Bound to the axial elements of sex chromosomes in pachytene spermatocytes. A small proportion of the protein is cytoplasmic, associated with Golgi stacks, By similarity. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9UNZ2
Gene Ontology
(Biological Process)
Complete annatation
Golgi organization [GO:0007030];
membrane fusion [GO:0061025];
nuclear envelope reassembly [GO:0031468];
proteasome-mediated ubiquitin-dependent protein catabolic process [GO:0043161]
Gene Ontology
(Molecular Function)
Complete annatation
phospholipid binding [GO:0005543];
ubiquitin binding [GO:0043130]
Gene Ontology
(Cellular Component)
Complete annatation
chromosome [GO:0005694];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
Golgi stack [GO:0005795];
intermediate filament cytoskeleton [GO:0045111];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
plasma membrane [GO:0005886];
VCP-NSFL1C complex [GO:1990730]
Protein-protein interaction121014
Phylogenetic treeQ9UNZ2
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.262884488438460.0001479582244985340.000682382606989569
AZA vs. DISU0.4968037378279220.05116494556534590.506741385973735
AZA vs. IL70.07605058974592470.6947033871406960.999311006273513
AZA vs. SAHA0.1081480876974280.6590019639010880.897305868831662
DISU vs. CD3-0.7798071858180420.03332454057887870.0779779765113346
DISU vs. IL7-0.4301773387031610.08966026821780230.396488279079897
DISU vs. SAHA-0.3861990051239170.187610951568410.554178983330306
DMSO vs. AZA-0.009049118960329570.9574161307827761
DMSO vs. CD3-1.283512047039677.82434179606994e-050.000361464916153921
DMSO vs. DISU-0.5079150213901630.03892873475646260.398272934677455
DMSO vs. IL70.09240705464662730.6102743160042230.915100640895645
DMSO vs. SAHA0.1111179295613770.6388317909059810.884108858477369
HIV vs. Mock in Activation0.06129972236146690.9215057617329210.999983755607037
HIV vs. Mock in Latency-0.007791827123584890.9627512604634610.999834320637052
IL7 vs. CD3-1.180083476029350.0002920168401784330.00146914880908195
SAHA vs. CD3-1.179122320624460.0009926949572146570.00356103618310518
SAHA vs. IL70.02942949413050410.9041717016149150.963726551109286
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.076767 0.665631
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.024 1.1 1.186 1.166 1.117
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1SS6 NMR - A=171-270.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04141 Protein processing in endoplasmic reticulum - Homo sapiens (human)