Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0006071
UniProt IDQ8TAT6
Primary gene name(s)NPLOC4
Synonym gene name(s)KIAA1499, NPL4
Protein nameNuclear protein localization protein 4 homolog
Protein functionThe ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope, By similarity. {ECO:0000250}.
Subcellular locationCytoplasm, cytosol {ECO:0000250}. Endoplasmic reticulum {ECO:0000250}. Nucleus {ECO:0000250}. Note=Associated with the endoplasmic reticulum and nuclear. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q8TAT6
Gene Ontology
(Biological Process)
Complete annatation
ER-associated ubiquitin-dependent protein catabolic process [GO:0030433];
error-free translesion synthesis [GO:0070987];
Golgi organization [GO:0007030];
retrograde protein transport, ER to cytosol [GO:0030970];
ubiquitin-dependent protein catabolic process [GO:0006511]
Gene Ontology
(Molecular Function)
Complete annatation
ubiquitin binding [GO:0043130];
ubiquitin protein ligase binding [GO:0031625];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
endoplasmic reticulum [GO:0005783];
nuclear outer membrane-endoplasmic reticulum membrane network [GO:0042175];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
UFD1-NPL4 complex [GO:0036501];
VCP-NPL4-UFD1 AAA ATPase complex [GO:0034098]
Protein-protein interaction120798
Phylogenetic treeQ8TAT6
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.6887359956199970.03599396445524590.0757788842393444
AZA vs. DISU0.5009919201929530.04847100328398860.494668048356799
AZA vs. IL7-0.01807717967694260.9249968710988780.999311006273513
AZA vs. SAHA-0.01198142903323140.9607849476719580.990779256780515
DISU vs. CD3-0.2016819298873560.5787540384896450.693602987453721
DISU vs. IL7-0.5282062848041670.0367288921003150.251072739157323
DISU vs. SAHA-0.5103349111134450.08068988338871920.361215374507988
DMSO vs. AZA0.06359295910644970.7039108569066371
DMSO vs. CD3-0.639300462606350.04608043394333580.0899691209637577
DMSO vs. DISU-0.4399765661278850.07263807356972830.502366116886556
DMSO vs. IL7-0.07397080925867210.6804574098054280.934851394110377
DMSO vs. SAHA-0.08060744925708910.7322487633539310.92166731787956
HIV vs. Mock in Activation0.09430790980102930.8793661890438320.999983755607037
HIV vs. Mock in Latency-0.08227888665580520.617551620725240.999834320637052
IL7 vs. CD3-0.7003567702408760.02975578551993130.0722857662950322
SAHA vs. CD3-0.7247577102572510.04100420112315380.0840839993095562
SAHA vs. IL70.002882399060195010.9905455879528070.996213782258613
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0834425 0.621594
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.023 1.023 1.114 1.163 1.055
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vpu cooperates with 20442859

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04141 Protein processing in endoplasmic reticulum - Homo sapiens (human)