Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0006036
UniProt IDQ96IV0
Primary gene name(s)NGLY1
Synonym gene name(s)PNG1
Protein namePeptide-N(4-(N-acetyl-beta-glucosaminylasparagine amidase
Protein functionSpecifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine, GlcNAc of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high-mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins. {ECO:0000269|PubMed:14749736, ECO:0000269|PubMed:15358861}.
Subcellular locationCytoplasm {ECO:0000269|PubMed:15358861}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q96IV0
Gene Ontology
(Biological Process)
Complete annatation
glycoprotein catabolic process [GO:0006516];
misfolded or incompletely synthesized protein catabolic process [GO:0006515];
protein deglycosylation [GO:0006517]
Gene Ontology
(Molecular Function)
Complete annatation
metal ion binding [GO:0046872];
peptide-N4-(N-acetyl-beta-glucosaminylasparagine amidase activity [GO:0000224]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737]
Protein-protein interaction120885
Phylogenetic treeQ96IV0
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.1056670883965560.7468889811462220.824274051683082
AZA vs. DISU-0.1104013193206220.6629511175274380.961528862271377
AZA vs. IL70.02802331603015070.8844626286974210.999311006273513
AZA vs. SAHA-0.5527422159242140.02433352640746530.174253480791512
DISU vs. CD3-0.2291455945523280.5272062421210620.648796382866592
DISU vs. IL70.1294776850395690.6076602857687280.879774341134179
DISU vs. SAHA-0.4410534265639890.1318741497605870.467091729974572
DMSO vs. AZA-0.02600619108997620.8772016784827321
DMSO vs. CD3-0.1427600774434490.6552411193465130.743593782520167
DMSO vs. DISU0.08273355483721890.7348758051346910.967658472195267
DMSO vs. IL70.06116863063790230.7345528314533010.946333083680773
DMSO vs. SAHA-0.5337464817343560.0244043502109840.161656352591629
HIV vs. Mock in Activation-0.1875884275468180.7630328880589610.999983755607037
HIV vs. Mock in Latency0.02397303618314740.8849345608746150.999834320637052
IL7 vs. CD3-0.06968180738598840.8282281999500780.888659144208964
SAHA vs. CD3-0.6832985731305770.05438566047123170.106233558431816
SAHA vs. IL7-0.5847302541078260.01705217624820290.0905086284385247
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.0806689 0.654553
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.027 0.918 0.803 0.756 0.891
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2CCQ X-ray 1.6Å A=11-109.
2CM0 X-ray 1.9Å A=11-109.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp160; precursor interacts with 22190034
Envelope transmembrane glycoprotein gp41 interacts with 22190034
HIV-1 virus replication enhanced by expression of human gene 18187620

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04141 Protein processing in endoplasmic reticulum - Homo sapiens (human)