Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0006019
UniProt IDQ13469
Primary gene name(s)NFATC2
Synonym gene name(s)NFAT1, NFATP
Protein nameNuclear factor of activated T-cells, cytoplasmic 2
Protein functionPlays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway. {ECO:0000269|PubMed:15790681, ECO:0000269|PubMed:21871017}.
Subcellular locationCytoplasm. Nucleus. Note=Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q13469
Gene Ontology
(Biological Process)
Complete annatation
B cell receptor signaling pathway [GO:0050853];
calcineurin-NFAT signaling cascade [GO:0033173];
cell migration [GO:0016477];
cellular response to DNA damage stimulus [GO:0006974];
cytokine production [GO:0001816];
Fc-epsilon receptor signaling pathway [GO:0038095];
myotube cell development [GO:0014904];
positive regulation of B cell proliferation [GO:0030890];
positive regulation of myoblast fusion [GO:1901741];
positive regulation of transcription, DNA-templated [GO:0045893];
regulation of transcription, DNA-templated [GO:0006355];
response to drug [GO:0042493]
Gene Ontology
(Molecular Function)
Complete annatation
chromatin binding [GO:0003682];
DNA binding [GO:0003677];
RNA polymerase II core promoter proximal region sequence-specific DNA binding [GO:0000978];
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding [GO:0001077];
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding [GO:0001078];
transcription factor activity, sequence-specific DNA binding [GO:0003700]
Gene Ontology
(Cellular Component)
Complete annatation
actin cytoskeleton [GO:0015629];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
intracellular ribonucleoprotein complex [GO:0030529];
nuclear transcription factor complex [GO:0044798];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
plasma membrane [GO:0005886]
Protein-protein interaction110846
Phylogenetic treeQ13469
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.1992724232808690.544275962373710.656949411779976
AZA vs. DISU-0.006577672265277890.9792443907747160.997980525085395
AZA vs. IL7-0.03125150857072360.8707221395067080.999311006273513
AZA vs. SAHA-0.04701454273485370.8469630412706550.963550011990275
DISU vs. CD30.1805995713737810.6182542760901140.726388539188026
DISU vs. IL7-0.03364515986565510.8936162171995760.98053444331394
DISU vs. SAHA-0.03898805669868020.8935032542786550.973945010373438
DMSO vs. AZA-0.0134159976389530.9360788846567911
DMSO vs. CD30.1746406765106360.5856191007667760.683691239387127
DMSO vs. DISU-0.008691599801518510.9715500723504950.995077522347534
DMSO vs. IL7-0.01046423353979340.9535225364688950.99170045075529
DMSO vs. SAHA-0.04020090284345710.8644290378530410.964308662433988
HIV vs. Mock in Activation0.05548888712639020.9291868047608650.999983755607037
HIV vs. Mock in Latency0.02094361618808280.8988449945211070.999834320637052
IL7 vs. CD30.1755874318824310.5850453205657360.703937135199784
SAHA vs. CD30.1274469041956090.7191748573128490.795563759796274
SAHA vs. IL7-0.01925996502290690.9368947836448940.976337381951253
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.298371 0.0188912
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.968 0.996 1.017 1.047 1.049
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1A02 X-ray 2.7Å N=392-678.
1OWR X-ray 3.0Å M/N/P/Q=396-678.
1P7H X-ray 2.6Å L/M/N/O=393-678.
1PZU X-ray 3.1Å B/D/H/I/L/M=396-678.
1S9K X-ray 3.1Å C=399-678.
2AS5 X-ray 2.7Å M/N=392-678.
2O93 X-ray 3.0Å L/M/O=396-678.
3QRF X-ray 2.8Å M/N=396-678.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat enhances 10207088
2013941916690925
220771407999066
10207088
Vpu regulates 25728138
Nef inhibits 21176845
24187576
Nef regulates 25728138
Envelope surface glycoprotein gp120 inhibits 7957556
16260021
Tat interacts with 22593154
reverse transcriptase enhanced by 9845362
Tat decreases phosphorylation of 12482669
Tat inhibited by 8834464
10207088
22077140
22422068
Vpr upregulates 12788643
Tat binds 10207088
Envelope surface glycoprotein gp120 activates 8814265
16260021
Nef activates 21176845
23227982
24187576
Tat requires 18097055

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04022 cGMP-PKG signaling pathway - Homo sapiens (human)
hsa04310 Wnt signaling pathway - Homo sapiens (human)
hsa04360 Axon guidance - Homo sapiens (human)
hsa04370 VEGF signaling pathway - Homo sapiens (human)
hsa04380 Osteoclast differentiation - Homo sapiens (human)
hsa04650 Natural killer cell mediated cytotoxicity - Homo sapiens (human)
hsa04658 Th1 and Th2 cell differentiation - Homo sapiens (human)
hsa04659 Th17 cell differentiation - Homo sapiens (human)
hsa04660 T cell receptor signaling pathway - Homo sapiens (human)
hsa04662 B cell receptor signaling pathway - Homo sapiens (human)
hsa04921 Oxytocin signaling pathway - Homo sapiens (human)
hsa05161 Hepatitis B - Homo sapiens (human)
hsa05166 HTLV-I infection - Homo sapiens (human)
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