Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0005624
UniProt IDQ9UKM7
Primary gene name(s)MAN1B1
Synonym gene name(s)unknown
Protein nameEndoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase
Protein functionInvolved in glycoprotein quality control targeting of misfolded glycoproteins for degradation. It primarily trims a single alpha-1,2-linked mannose residue from Man(9GlcNAc(2 to produce Man(8GlcNAc(2, but at high enzyme concentrations, as found in the ER quality control compartment, ERQC, it further trims the carbohydrates to Man(5-6GlcNAc(2. {ECO:0000269|PubMed:12090241, ECO:0000269|PubMed:18003979}.
Subcellular locationEndoplasmic reticulum membrane {ECO:0000269|PubMed:10409699};
Single-pass type II membrane protein {ECO:0000269|PubMed:10409699}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9UKM7
Gene Ontology
(Biological Process)
Complete annatation
endoplasmic reticulum mannose trimming [GO:1904380];
ER-associated ubiquitin-dependent protein catabolic process [GO:0030433];
mannose trimming involved in glycoprotein ERAD pathway [GO:1904382];
N-glycan processing [GO:0006491];
oligosaccharide metabolic process [GO:0009311];
protein alpha-1,2-demannosylation [GO:0036508];
trimming of terminal mannose on B branch [GO:0036509]
Gene Ontology
(Molecular Function)
Complete annatation
alpha-mannosidase activity [GO:0004559];
calcium ion binding [GO:0005509];
mannosyl-oligosaccharide 1,2-alpha-mannosidase activity [GO:0004571]
Gene Ontology
(Cellular Component)
Complete annatation
endoplasmic reticulum [GO:0005783];
endoplasmic reticulum membrane [GO:0005789];
endoplasmic reticulum quality control compartment [GO:0044322];
extracellular vesicle [GO:1903561];
Golgi apparatus [GO:0005794];
integral component of membrane [GO:0016021];
membrane [GO:0016020]
Protein-protein interaction116414
Phylogenetic treeQ9UKM7
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.9834950129287540.002972751405075910.00934612206451902
AZA vs. DISU-0.08873705900352050.7257599887557490.974401202035083
AZA vs. IL7-0.2064712222383440.2836981564291410.999311006273513
AZA vs. SAHA-0.1229135115465890.6146717263577840.874803388102138
DISU vs. CD30.8819621633699890.01597732912453010.0427328352063816
DISU vs. IL7-0.1273443182220170.6134428781361490.88198868744897
DISU vs. SAHA-0.03176321184433150.9132718138724950.98054566551773
DMSO vs. AZA0.1103058863770330.510893109157091
DMSO vs. CD31.080128585795870.000845408036068140.00295431966211239
DMSO vs. DISU0.1965829598407430.420692079942550.877050108852108
DMSO vs. IL7-0.3091091586611110.08621826907102090.548940653907166
DMSO vs. SAHA-0.2381273955648150.3127716694415350.663962137427825
HIV vs. Mock in Activation0.05735761793672750.926757408256060.999983755607037
HIV vs. Mock in Latency0.125306061064380.4478063176445580.999834320637052
IL7 vs. CD30.7827960971208680.01550826852292190.0427107859393388
SAHA vs. CD30.8368284992783220.01866473902919110.04346106744617
SAHA vs. IL70.08055443146174790.7409075864221460.881955851869091
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.642986 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.144 1.235 1.343 1.304 1.057
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB01955 1,4-Butanediol experimental unknown unknown
DB02422 Methyl-2-S-(Alpha-D-Mannopyranosyl)-2-Thio-Alpha-D-Mannopyranoside experimental unknown unknown
DB02742 Kifunensine experimental unknown unknown
DB03206 Duvoglustat experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1FMI X-ray 1.9Å A=243-697.
1FO2 X-ray 2.3Å A=243-699.
1FO3 X-ray 1.7Å A=243-699.
1X9D X-ray 1.4Å A=172-699.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp120 processed by 12560567
Envelope surface glycoprotein gp120 interacts with 26205822
Envelope transmembrane glycoprotein gp41 processed by 1736542
Envelope surface glycoprotein gp160; precursor processed by 2187500

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00510 N-Glycan biosynthesis - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa04141 Protein processing in endoplasmic reticulum - Homo sapiens (human)